Rapid detection of several endangered agarwood-producing Aquilaria species and their potential adulterants using plant DNA barcodes coupled with high-resolution melting (Bar-HRM) analysis
Abstract
Aquilaria is an endangered agarwood-producing genus that is currently protected by international laws. The agarwood trade is strictly monitored to prevent illegal harvesting, which has caused high demand for this natural product. Other plant sources of similar appearance or fragrance as agarwood are used as adulterant species in counterfeit products. To promote species identification via the DNA barcoding technique, the existing DNA barcoding database in our laboratory was enriched with seven plant barcoding sequences from a commercially important Aquilaria species (Aquilaria beccariana) and seven adulterant species (Cocos nucifera, Dalbergia latifolia, Pinus contorta var. latifolia, Santalum album, Strychnos ignatii, Thuja sp. and Terminalia catappa). DNA barcoding with high-resolution melting analysis (Bar-HRM) showed that the mini-barcode internal transcribed spacer 1 (ITS1) was an effective gene locus that allows for a rapid and species-specific detection of Aquilaria and their adulterants, while four other mini-barcodes (rbcL, trnL intron, ITS2 and 5.8s) functioned as a support and a crosscheck for the barcoding results. The accuracy of the Bar-HRM technique in species origin identification was further assessed with seven agarwood blind specimens. The Bar-HRM technique is a potential tool for validating agarwood-species origin and detecting products with adulterant species.
Acknowledgments
The authors thank Dr. Rita Kartika Sari from Bogor Agricultural University (IPB) and Dr. Mohd Noor Mahat from Forest Research Institute Malaysia (FRIM) for sharing plant specimens. We acknowledge Mr. Samsuddin Ahmad Syazwan from Universiti Putra Malaysia (UPM) for assisting in artwork. We also thank the anonymous reviewers, whose comments have greatly improved this manuscript. This work was supported by the Ministry of Education of Malaysia (FRGS/1/2016/WAB07/UPM/02/3).
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Accessing the natural variation of the abundances of major lignans in the heartwood of Taiwania cryptomerioides by 1H-NMR and LC-MS profiling
- Rapid detection of several endangered agarwood-producing Aquilaria species and their potential adulterants using plant DNA barcodes coupled with high-resolution melting (Bar-HRM) analysis
- Changes in sorption and electrical properties of wood caused by fungal decay
- Effect of hardening parameters of wood preservatives based on tannin copolymers
- In situ polymerization of 2-hydroxyethyl methacrylate (HEMA) and 3-(methacryloxy)propyltrimethoxysilane (MAPTES) in poplar cell wall to enhance its dimensional stability
- Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents
- Towards better UV-blocking and antioxidant performance of varnish via additives based on lignin and its colloids
- Thermal stability of lignin in ground pulp (GP) and the effect of lignin modification on GP’s thermal stability: TGA experiments with dimeric lignin model compounds and milled wood lignins
- Preparation, structural changes and adsorption performance of heavy metal ions on sulfonated cellulose with varying degrees of substitution
- Mass transport and yield during spinning of lignin-cellulose carbon fiber precursors
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Accessing the natural variation of the abundances of major lignans in the heartwood of Taiwania cryptomerioides by 1H-NMR and LC-MS profiling
- Rapid detection of several endangered agarwood-producing Aquilaria species and their potential adulterants using plant DNA barcodes coupled with high-resolution melting (Bar-HRM) analysis
- Changes in sorption and electrical properties of wood caused by fungal decay
- Effect of hardening parameters of wood preservatives based on tannin copolymers
- In situ polymerization of 2-hydroxyethyl methacrylate (HEMA) and 3-(methacryloxy)propyltrimethoxysilane (MAPTES) in poplar cell wall to enhance its dimensional stability
- Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents
- Towards better UV-blocking and antioxidant performance of varnish via additives based on lignin and its colloids
- Thermal stability of lignin in ground pulp (GP) and the effect of lignin modification on GP’s thermal stability: TGA experiments with dimeric lignin model compounds and milled wood lignins
- Preparation, structural changes and adsorption performance of heavy metal ions on sulfonated cellulose with varying degrees of substitution
- Mass transport and yield during spinning of lignin-cellulose carbon fiber precursors