Differential proteome analysis of the extracts from the xylem of Cinnamomum camphora inhibiting Coriolus versicolor
-
Quan Li
,
Liping Xu
und
Xin Guan
Abstract
The proteins of Coriolus versicolor treated with the acetone extracts of Cinnamomum camphora xylem has been investigated. The specific proteins found in the extract treated C. versicolor, were characterized by two-dimensional (2D) gel electrophoresis (2D-GE) and identified by matrix assisted laser desorption ionization (MALDI) combined with tandem time-of-flight microscopy, i.e. TOF-MS/TOF-MS. Most of 474 proteins detected from the pure culture were common metabolic proteins. Nineteen protein spots changed significantly and 17 spots were identified successfully. The 19 proteins were assigned to 10 functional groups including ion binding, translation factor activity, nucleic acid binding and ATPase activity. These proteins are involved in signal transduction, transport and membrane trafficking. The down regulated expression of translation elongation factor 1 alpha (EF1α), α-tubulin, and tropomyosin-1 showed that protein translation elongation, growth-stimulating signal transduction, signal transduction and material transport were suppressed, which resulted in the apoptosis of the pathogen. These findings provide a better understanding of the anti-fungal mechanism of C. camphora and indicate the way for the development of wood preservatives based on natural extracts.
Acknowledgments
This research was supported by the Natural Science Foundation of China (No. 31760191) and Fujian Agriculture and Forestry University of Science and Technology Innovation Fund Project (No. CXZX2016007).
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
References
Abbas, A., Koc, H., Liu, F., Tien, M. (2005) Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate. Curr. Genet. 47:49–56.10.1007/s00294-004-0550-4Suche in Google Scholar PubMed
Almeida, J.S., Stanislaus, R., Krug, E., Arthur, J.M. (2005) Normalization and analysis of residual variation in two-dimensional gel electrophoresis for quantitative differential proteomics. Proteomics 5:1242–1249.10.1002/pmic.200401003Suche in Google Scholar PubMed
Asina, F., Brzonova, I., Voeller, K., Kozliak, E., Kubátová, A., Yao, B., Ji, Y. (2016) Biodegradation of lignin by fungi, bacteria and laccases. Bioresour. Technol. 220:414–424.10.1016/j.biortech.2016.08.016Suche in Google Scholar PubMed
Bader, T.K., Hofstetter, K., Alfredsen, G., Bollmus, S. (2016) Microstructure and stiffness of Scots pine (Pinus sylvestris L) sapwood degraded by Gloeophyllum trabeum and Trametes versicolor – Part I: changes in chemical composition, density and equilibrium moisture content. Holzforschung 66:191–198.10.1515/HF.2011.149Suche in Google Scholar
Bhardwaj, S.K., Singla, S.K., Bhardwaj, R.K. (2012) Evaluation of plant extracts as antifungal agents against wood rotting fungi Coriolus versicolor (L.: Fr.) Quelet. J. Ind. Wood Sci. 9:62–65.10.1007/s13196-012-0065-4Suche in Google Scholar
Campo, S., Carrascal, M., Coca, M., Abián, J., Segundo, B.S. (2004) The defense response of germinating maize embryos against fungal infection: a proteomics approach. Proteomics 4:383–396.10.1002/pmic.200300657Suche in Google Scholar PubMed
Condeelis, J. (1995) Elongation factor 1 alpha, translation and the cytoskeleton. Trends Biochem. Sci. 20:169–170.10.1016/S0968-0004(00)88998-7Suche in Google Scholar
De Filippis, L., Magel, E. (2012) Identification of biochemical differences between the sapwood and transition zone in Robinia pseudoacacia L. by differential display of proteins. Holzforschung 66:543–549.10.1515/hf.2011.178Suche in Google Scholar
Ferraris, D.M., Spallek, R., Oehlmann, W., Singh, M., Rizzi, M. (2015) Structures of citrate synthase and malate dehydrogenase of Mycobacterium tuberculosis. Proteins 83:389–394.10.1002/prot.24743Suche in Google Scholar PubMed
Gagat, M., Grzanka, D., Izdebska, M., Sroka, W.D., Hałaswiśniewska, M., Grzanka, A. (2016) Tropomyosin-1 protects transformed alveolar epithelial cells against cigaret smoke extract through the stabilization of F-actin-dependent cell-cell junctions. Acta Histochem. 118:225–235.10.1016/j.acthis.2016.01.003Suche in Google Scholar PubMed
Gangwani, L., Mikrut, M., Galchevagargova, Z., Davis, R.J. (1998) Interaction of ZPR1 with translation elongation factor-1α in proliferating cells. J. Cell Biol. 143:1471–1484.10.1083/jcb.143.6.1471Suche in Google Scholar PubMed PubMed Central
Giannakakou, P., Dan, S., Fojo, T. (2000) Tubulin/microtubules: still a promising target for new chemotherapeutic agents. J. Natl. Cancer I. 92:182–183.10.1093/jnci/92.3.182Suche in Google Scholar PubMed
Guo, S., Geng, Z., Zhang, W., Liang, J., Wang, C., Deng, Z., Du, S. (2016) The chemical composition of essential oils from Cinnamomum camphora and their insecticidal activity against the stored product pests. Int. J. Mol. Sci. 17:1836.10.3390/ijms17111836Suche in Google Scholar PubMed PubMed Central
Gururaja, T., Li, W., Noble, W.S., Payan, D.G., Anderson, D.C. (2003) Multiple functional categories of proteins identified in an in vitro cellular ubiquitin affinity extract using shotgun peptide sequencing. J. Proteome Res. 2:394–404.10.1021/pr034019nSuche in Google Scholar PubMed
Heilmann-Clausen, J., Boddy, L. (2005) Inhibition and stimulation effects in communities of wood decay fungi: exudates from colonized wood influence growth by other species. Microbial Ecol. 49:399–406.10.1007/s00248-004-0240-2Suche in Google Scholar PubMed
Hibbett, D.S., Donoghue, M.J. (2001) Analysis of character correlations among wood decay mechanisms, mating systems, and substrate ranges in homobasidiomycetes. Syst. Biol. 50:215–242.10.1080/10635150151125879Suche in Google Scholar
Highley, T.L. (2011) Influence of type and amount of lignin on decay by Coriolus versicolor. Can. J. For. Res. 12:435–438.10.1139/x82-065Suche in Google Scholar
Hsu, K.-H., Huang, W.-K., Lin, Y.-L., Chang, S.-T., Chu, F.-H. (2012) A genetic marker of 4-coumarate: coenzyme a ligase gene in the cinnamaldehyde-chemotype Cinnamomum osmophloeum. Holzforschung 66:897–904.10.1515/hf-2012-0010Suche in Google Scholar
Hung, K.-H., Lin, C.-H., Ju, L.-P. (2017) Tracking the geographical origin of timber by DNA fingerprinting: a study of the endangered species Cinnamomum kanehirae in Taiwan. Holzforschung 71:853–862.10.1515/hf-2017-0026Suche in Google Scholar
Jiang, H., Wang, J., Song, L., Cao, X., Yao, X., Tang, F., Yue, Y. (2016) GC×GC-TOFMS analysis of essential oils composition from leaves, twigs and seeds of Cinnamomum camphora L. Presl and their insecticidal and repellent activities. Molecules 21:423.10.3390/molecules21040423Suche in Google Scholar PubMed PubMed Central
Kang, Y.M., Prewitt, M.L., Diehl, S.V. (2009) Proteomics for biodeterioration of wood (Pinus taeda L.): challenging analysis by 2-D PAGE and MALDI-TOF/TOF/MS. Int. Biodeterior. Biodegrad. 63:1036–1044.10.1016/j.ibiod.2009.07.008Suche in Google Scholar
Kim, H.I., Kwon, O., Kong, W.S., Lee, C.S., Park, Y.J. (2014) Genome-wide identification and characterization of novel laccase genes in the white-rot fungus Flammulina velutipes. Mycobiology 42:322–330.10.5941/MYCO.2014.42.4.322Suche in Google Scholar PubMed PubMed Central
Lam, S.K., Ng, T.B. (2002) Pananotin, a potent antifungal protein from roots of the traditional chinese medicinal herb Panax notoginseng. Planta Med. 68:1024–1028.10.1055/s-2002-35649Suche in Google Scholar PubMed
Levasseur, A., Lomascolo, A., Chabrol, O., Ruizdueñas, F.J., Boukhrisuzan, E., Piumi, F., Kües, U., Ram, A.F.J., Murat, C., Haon, M. (2014) The genome of the white-rot fungus Pycnoporus cinnabarinus: a basidiomycete model with a versatile arsenal for lignocellulosic biomass breakdown. BMC Genomics 15:1–24.10.1186/1471-2164-15-486Suche in Google Scholar PubMed PubMed Central
Li, Q., Lin, J.G., Liu, J. (2013) Decay resistance of wood treated with extracts of Cinnamomum camphora xylem. Bioresources 8:4208–4217.10.15376/biores.8.3.4208-4217Suche in Google Scholar
Li, Q., Wang, X.X., Lin, J.G., Liu, J., Jiang, M.S., Chu, L.X. (2014) Chemical composition and antifungal activity of extracts from the xylem of Cinnamomum camphora. Bioresources 9:383–399.10.15376/biores.9.2.2560-2571Suche in Google Scholar
L’Hostis, C., Thévenon, M.-F., Fredon, E., Gérardin, P. (2018) Improvement of beech wood properties by in situ formation of polyesters of citric and tartaric acid in combination with glycerol. Holzforschung. 72:291–299.10.1515/hf-2017-0081Suche in Google Scholar
Manavalan, T., Manavalan, A., Heese, K. (2015) Characterization of lignocellulolytic enzymes from white-rot fungi. Curr. Microbiol. 70:485–498.10.1007/s00284-014-0743-0Suche in Google Scholar PubMed
Martinez, D., Challacombe, J., Morgenstern, I., Hibbett, D., Schmoll, M., Kubicek, C.P., Ferreira, P., Ruizduenas, F.J., Martinez, A.T., Kersten, P. (2009) Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion. Proc. Natl Acad Sci. USA 106:1954–1959.10.1073/pnas.0809575106Suche in Google Scholar PubMed PubMed Central
Matsuzaki, F., Shimizu, M., Wariishi, H. (2008) Proteomic and metabolomic analyses of the white-rot fungus Phanerochaete chrysosporium exposed to exogenous benzoic acid. J. Proteome Res. 7:2342–2350.10.1021/pr700617sSuche in Google Scholar PubMed
Murat, C., Payen, T., Petitpierre, D., Labbé, J. (2013) Repeated elements in filamentous fungi with a focus on wood‐decay fungi. In: The Ecological Genomics of Fungi. John Wiley & Sons, Hoboken, pp.21–40.10.1002/9781118735893.ch2Suche in Google Scholar
Oliveira, J.M.P.F.D., Graaff, L.H.D. (2011) Proteomics of industrial fungi: trends and insights for biotechnology. Appl. Microbiol. Biotechnol. 89:225–237.10.1007/s00253-010-2900-0Suche in Google Scholar PubMed PubMed Central
Pérez, J., Muñoz-Dorado, J., Rubia, T.D.L., Martínez, J. (2002) Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview. Int. Microbiol. 5:53–63.10.1007/s10123-002-0062-3Suche in Google Scholar PubMed
Salvachúa, D., Martínez, A.T., Ming, T., López-Lucendo, M.F., García, F., Ríos, V.D.L., Martínez, M.J., Prieto, A. (2013) Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment. Biotechnol. Biofuels 6:1–14.10.1186/1754-6834-6-115Suche in Google Scholar PubMed PubMed Central
Singh, D., Chen, S. (2008) The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes. Appl. Microbiol. Biotechnol. 81:399–417.10.1007/s00253-008-1706-9Suche in Google Scholar PubMed
Song, Y., Wynn, J.P., Li, Y., Grantham, D., Ratledge, C. (2001) A pre-genetic study of the isoforms of malic enzyme associated with lipid accumulation in Mucor circinelloides. Microbiology 147:1507–1515.10.1099/00221287-147-6-1507Suche in Google Scholar PubMed
Ujor, V.C., Peiris, D.G., Monti, M., Kang, A.S., Clements, M.O., Hedger, J.N. (2012) Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune, to the biocontrol fungus, Trichoderma viride. Lett. Appl. Microbiol. 54:336–343.10.1111/j.1472-765X.2012.03215.xSuche in Google Scholar PubMed
Wang, Y.Z., Sun, S.Q., Zhou, Y.B. (2011) Extract of the dried heartwood of Caesalpinia sappan L. attenuates collagen-induced arthritis. J. Ethnopharmacol. 136:271–278.10.1016/j.jep.2011.04.061Suche in Google Scholar PubMed
Wu, C.-C., Chu, F.-H., Ho, C.-K., Sung, C.-H., Chang, S.-H. (2017) Comparative analysis of the complete chloroplast genomic sequence and chemical components of Cinnamomum micranthum and Cinnamomum kanehirae. Holzforschung 71:189–197.10.1515/hf-2016-0133Suche in Google Scholar
Wynn, J.P., Bin, A.H.A., Ratledge, C. (1999) The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi. Microbiology 145:1911–1917.10.1099/13500872-145-8-1911Suche in Google Scholar PubMed
Yıldırım, V., Özcan, S., Becher, D., Büttner, K., Hecker, M., Özcengiz, G. (2011) Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure. Proteome Sci. 9:1–15.10.1186/1477-5956-9-12Suche in Google Scholar PubMed PubMed Central
©2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Improvement of enzymatic digestibility of wood by a sequence of optimized milling procedures with final vibratory tube mills for the amorphization of cellulose
- The effect of delignification on the properties of cellulosic fiber material
- Optimization of polyol production via liquefaction from Acacia mangium and analysis of the polyols by traditional methods and two-dimensional correlation spectroscopy
- Differential proteome analysis of the extracts from the xylem of Cinnamomum camphora inhibiting Coriolus versicolor
- Review
- Nondestructive assessment and imaging methods for internal inspection of timber. A review.
- Effects of sample size on swelling and shrinkage of Larix kaempferi and Cryptomeria japonica as determined by digital caliper, image analysis, and digital image correlation (DIC)
- Preparation of a synergistically stabilized oil-in-water paraffin Pickering emulsion for potential application in wood treatment
- Improved wood properties via two-step grafting with itaconic acid (IA) and nano-SiO2
- Shear moduli in the longitudinal-radial and radial-tangential planes of Sitka spruce measured by torsional vibration tests
- Creep behavior of 3D core wood-strand sandwich panels
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Improvement of enzymatic digestibility of wood by a sequence of optimized milling procedures with final vibratory tube mills for the amorphization of cellulose
- The effect of delignification on the properties of cellulosic fiber material
- Optimization of polyol production via liquefaction from Acacia mangium and analysis of the polyols by traditional methods and two-dimensional correlation spectroscopy
- Differential proteome analysis of the extracts from the xylem of Cinnamomum camphora inhibiting Coriolus versicolor
- Review
- Nondestructive assessment and imaging methods for internal inspection of timber. A review.
- Effects of sample size on swelling and shrinkage of Larix kaempferi and Cryptomeria japonica as determined by digital caliper, image analysis, and digital image correlation (DIC)
- Preparation of a synergistically stabilized oil-in-water paraffin Pickering emulsion for potential application in wood treatment
- Improved wood properties via two-step grafting with itaconic acid (IA) and nano-SiO2
- Shear moduli in the longitudinal-radial and radial-tangential planes of Sitka spruce measured by torsional vibration tests
- Creep behavior of 3D core wood-strand sandwich panels
