Home Physical Sciences A genetic marker of 4-coumarate: coenzyme A ligase gene in the cinnamaldehyde-chemotype Cinnamomum osmophloeum
Article
Licensed
Unlicensed Requires Authentication

A genetic marker of 4-coumarate: coenzyme A ligase gene in the cinnamaldehyde-chemotype Cinnamomum osmophloeum

  • Keng-Hao Hsu , Wen-Ke Huang , Yan-Liang Lin , Shang-Tzen Chang and Fang-Hua Chu EMAIL logo
Published/Copyright: April 19, 2012
Holzforschung
From the journal Holzforschung Volume 66 Issue 7

Abstract

There are numerous chemotypes of Cinnamomum osmophloeum in Taiwan, each of which generates an identical profile of volatile secondary metabolites. Cinnamaldehyde is the major constituent of C. osmophloeum and its quantity varies between strains. The cinnamaldehyde-type C. osmophloeum contains abundance of cinnamaldehyde, which is an economically important product, which can be gained from the leaf essential oil. Here, the genes involved in cinnamaldehyde biosynthesis have been investigated and four candidate genes, phenylalanine ammonia-lyase (CoPAL), 4-coumarate: coenzyme A ligase 1 and 4 (Co4CL1 and Co4CL4), and cinnamoyl-CoA reductase (CoCCR), were selected as potential molecular marker typical for the cinnamaldehyde chemotype. Cinnamaldehyde was increased in Co4CL1, Co4CL4, and CoCCR transgenic plants. The results showed that the cinnamaldehyde and non-cinnamaldehyde chemotypes can be distinguished by a single nucleotide polymorphism in the substrate binding pocket region of Co4CL4, at residue 378 of Co4CL4. This polymorphism could be used as a potential molecular marker for identification of strains of C. osmophloeum, which belong to high-yield cinnamaldehyde producer type. In addition, this finding might provide a suitable strategy for biosynthesis of bioactive metabolites in the future.

Keywords: cinnamaldehyde; Cinnamomum osmophloeum; cinnamoyl-CoA reductase; 4-coumarate: coenzyme A ligase; phenylalanine ammonia-lyase; single nucleotide polymorphism
Corresponding author. School of Forestry and Resource Conservation, National Taiwan University, No.1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan Phone: +886-2-33665261 Fax: +886-2-23654520
Received: 2012-1-21
Accepted: 2012-3-22
Published Online: 2012-04-19
Published in Print: 2012-10-01

©2012 by Walter de Gruyter Berlin Boston

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Review
  4. Comparative evaluation of different kinetic models for batch cooking: A review
  5. Original Papers
  6. Characteristics of prehydrolysis-kraft pulp fibers from Scots pine
  7. Treating birch wood with a switchable 1,8-diazabicyclo-[5.4.0]-undec-7-ene-glycerol carbonate ionic liquid
  8. Optical properties of thermomechanical pulp (TMP) obtained from sulfite-pretreated Norway spruce with focus on two-photon spectral imaging (TPSI)
  9. Physicochemical properties of sodium lignosulfonates (NaLS) modified by laccase
  10. Determination of metal ions in single wood fiber by LA-ICP-MS
  11. Hydrolytic enzyme activity of EN113 standard basidiomycetes in the fermentation of lignocellulosic material and wood colonization
  12. Influence of the viscosity of UF resins on the radial and tangential penetration into poplar wood and on the shear strength of adhesive joints
  13. Development of soy-based adhesives for the manufacture of wood composite products
  14. Exploratory study on fatigue behaviour of laterally loaded, nailed timber joints, based on a dissipated energy criterion
  15. Young’s and shear moduli of glued laminated timber composed of different species obtained by a flexural vibration test
  16. Heartwood detection for Scotch pine by fluorescence image analysis
  17. The antagonistic properties of Trichoderma spp. inhabiting woods for potential biological control of wood-damaging fungi
  18. Investigation of copper solubilization and reaction in micronized copper treated wood by electron paramagnetic resonance (EPR) spectroscopy
  19. A genetic marker of 4-coumarate: coenzyme A ligase gene in the cinnamaldehyde-chemotype Cinnamomum osmophloeum
  20. Meetings
  21. Meetings
https://doi.org/10.1515/hf-2012-0010
Keywords for this article
cinnamaldehyde; Cinnamomum osmophloeum; cinnamoyl-CoA reductase; 4-coumarate: coenzyme A ligase; phenylalanine ammonia-lyase; single nucleotide polymorphism

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Review
  4. Comparative evaluation of different kinetic models for batch cooking: A review
  5. Original Papers
  6. Characteristics of prehydrolysis-kraft pulp fibers from Scots pine
  7. Treating birch wood with a switchable 1,8-diazabicyclo-[5.4.0]-undec-7-ene-glycerol carbonate ionic liquid
  8. Optical properties of thermomechanical pulp (TMP) obtained from sulfite-pretreated Norway spruce with focus on two-photon spectral imaging (TPSI)
  9. Physicochemical properties of sodium lignosulfonates (NaLS) modified by laccase
  10. Determination of metal ions in single wood fiber by LA-ICP-MS
  11. Hydrolytic enzyme activity of EN113 standard basidiomycetes in the fermentation of lignocellulosic material and wood colonization
  12. Influence of the viscosity of UF resins on the radial and tangential penetration into poplar wood and on the shear strength of adhesive joints
  13. Development of soy-based adhesives for the manufacture of wood composite products
  14. Exploratory study on fatigue behaviour of laterally loaded, nailed timber joints, based on a dissipated energy criterion
  15. Young’s and shear moduli of glued laminated timber composed of different species obtained by a flexural vibration test
  16. Heartwood detection for Scotch pine by fluorescence image analysis
  17. The antagonistic properties of Trichoderma spp. inhabiting woods for potential biological control of wood-damaging fungi
  18. Investigation of copper solubilization and reaction in micronized copper treated wood by electron paramagnetic resonance (EPR) spectroscopy
  19. A genetic marker of 4-coumarate: coenzyme A ligase gene in the cinnamaldehyde-chemotype Cinnamomum osmophloeum
  20. Meetings
  21. Meetings
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2026 De Gruyter Brill
Downloaded on 11.2.2026 from https://www.degruyterbrill.com/document/doi/10.1515/hf-2012-0010/html
Scroll to top button