Abstract
Orobanche cumana Wallr. is a holoparasitic plant for only sunflower, hence it is called as sunflower broomrape. Yield loss created by O. cumana which is generally 50 % can reach to 100 %. In this study, it was planned to perform molecular characterization of O. cumana germplasm as nine locations of Thrace region obtained from Trakya Agricultural Research Institute by using Single Nucleotide Polymorphism (SNP) markers, widely used in plant breeding programs, in Competitive Allele Specific PCR (KASP) assay which is a fluorescent tagged allele specific PCR method based, economic, reliable and easily repeatable genotyping technology. Databases and literature were scanned to spot variations on O. cumana genome which is not known clearly. So far, four SSR (Simple Sequence Repeat) marker (Ocum-197, Ocum-006, Ocum-023 and Ocum-151) regions showing polymorphic pattern were used for searching possible SNPs. Primer pairs were designed for amplification of the regions possibly having SNPs and PCR amplifications with these primer pairs were performed and 1 candidate deletion was detected on the amplicon which was amplified by Ocum-197 SSR marker. Following, the deletion was converted to KASP primers and KASP assay was performed. The deletion marker, Del-197, has grouped the samples from nine locations in the resulting allelic discrimination plot and infestation was performed according to this grouping, As a conclusion, Del-197 is considered as a selective marker for the ability to rapidly assay allelic variation at DNA markers for O. cumana populations that have effects on infestation results were evaluated as races, F, G, H and I in Thrace region.
Acknowledgements
The authors would like to thank Dr Atilla Goknur from GGT Global Genetics for the help of the configuration of KASP assay and discussions of results. This study was performed with the support of Marmara University Research Foundation; Project ID: FEN-C-YLP-090217-0050.
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© 2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Review Paper
- Chao-Chien Jan: Thirty-five Years of Dedicated Research Utilizing Wild Sunflower Crop Relatives for Sunflower Improvement
- Research Articles
- Helianthus porteri, a Granite Outcrop Endemic, Does Not Have More Drought Resistant Traits Than Congeners
- Effect of Sclerotinia sclerotiorum on Sunflower Seeds Quality
- Genomic Evaluation of Sunflower Broomrape (Orobanche Cumana) Germplasm by KASP Assay
- Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower
- Selection for Some Functional Markers for Adaptability of Helianthus argophyllus × Helianthus annuus Derived Population under Abiotic Stress Conditions
- Inheritance of Basal Branching in Sunflower
- Diversity of Fungi in Rhizoplane, Rhizosphere and Edaphosphere of Sunflower at Different Stages of its Development
- Performance and Water-Use Efficiency of Wild Cytoplasmic Sources in Sunflower
Artikel in diesem Heft
- Frontmatter
- Review Paper
- Chao-Chien Jan: Thirty-five Years of Dedicated Research Utilizing Wild Sunflower Crop Relatives for Sunflower Improvement
- Research Articles
- Helianthus porteri, a Granite Outcrop Endemic, Does Not Have More Drought Resistant Traits Than Congeners
- Effect of Sclerotinia sclerotiorum on Sunflower Seeds Quality
- Genomic Evaluation of Sunflower Broomrape (Orobanche Cumana) Germplasm by KASP Assay
- Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower
- Selection for Some Functional Markers for Adaptability of Helianthus argophyllus × Helianthus annuus Derived Population under Abiotic Stress Conditions
- Inheritance of Basal Branching in Sunflower
- Diversity of Fungi in Rhizoplane, Rhizosphere and Edaphosphere of Sunflower at Different Stages of its Development
- Performance and Water-Use Efficiency of Wild Cytoplasmic Sources in Sunflower