Effects of tumor necrosis factor-α rs1800629 and interleukin-10 rs1800872 genetic variants on type 2 diabetes mellitus susceptibility and metabolic parameters among Jordanians

Lana Nasrallah Mousa; Yazun Jarrar; Munir Gharaibeh; Hussam Alhawari

doi:10.1515/dmpt-2024-0002

Article

Effects of tumor necrosis factor-α rs1800629 and interleukin-10 rs1800872 genetic variants on type 2 diabetes mellitus susceptibility and metabolic parameters among Jordanians

Lana Nasrallah Mousa , Yazun Jarrar , Munir Gharaibeh and Hussam Alhawari

Published/Copyright: May 14, 2024

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From the journal Drug Metabolism and Personalized Therapy Volume 39 Issue 2

Abstract

Objectives

Diabetes mellitus (DM) is a complex chronic illness with diverse pathogenesis and associations with health complications. Genetic factors significantly contribute to DM development, and tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) genes play major roles. This study aims to explore the influence of TNF-α rs1800629 and IL-10 rs1800872 genetic variants on T2DM development in Jordanian patients at Jordan University Hospital.

Methods

One-hundred and 60 diabetic and 159 non-diabetic subjects were genotyped for TNF-α rs1800629. Additionally, 181 diabetic and 191 non-diabetic subjects were genotyped for IL-10 rs1800872 using PCR-RFLP genotyping method. The demographic, lipid, and glycemic parameters of the patients were obtained from the computer records in the hospital.

Results

TNF-α rs1800629 and IL-10 rs1800872 genetic variants exhibited significant different frequencies in non-T2DM subjects and T2DM patients. The difference in TNF-α rs1800629 genotype frequency between non-T2DM and T2DM participants was significant under the dominant model, while the IL-10 rs1800872 genotype frequency was significant under the recessive model. A significant association (p<0.05) was observed between TNF-α rs1800629 and total cholesterol levels, and between IL-10 rs1800872 polymorphism and glycosylated hemoglobin (HbA_1c) and creatinine levels among T2DM patients.

Conclusions

TNF-α rs1800629 and IL-10 rs1800872 are identified as genetic risk factors for T2DM. These variants also correlate with variations in cholesterol, HbA_1c, and creatinine levels among T2DM patients. Larger clinical studies are warranted to validate these findings.

Keywords: diabetes; TNF-α; IL-10; Jordanians; genetic variants

Corresponding author: Yazun Jarrar, PhD, Department of Basic Medical Sciences Faculty of Medicine, Al-Balqa Applied University, Al-Salt 19117, Jordan, E-mail: yazan.jarrar@bau.edu.jo

Funding source: University of Jordan

Award Identifier / Grant number: Unassigned

Acknowledgments

The authors would like to thank the University of Jordan for supporting this research.

Research ethics: The protocol of this study has been approved by the Ethical Committe in the University of Jordan on 7/2020 with reference number of JU-72635.
Informed consent: Informed consent was obtained from all individuals included in this study.
Author contributions: Lina wrote the manuscript, Yazun and Munir were the supervisors of this research. Hussam was the physician who diagnosed and collected the clinical data. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: The authors state no conflict of interest.
Research funding: This research has been funded by the University of Jordan.
Data availability: The raw data can be obtained on request from the corresponding author.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/dmpt-2024-0002).

Received: 2024-01-11

Accepted: 2024-04-01

Published Online: 2024-05-14

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Articles in the same Issue

https://doi.org/10.1515/dmpt-2024-0002

Keywords for this article

diabetes; TNF-α; IL-10; Jordanians; genetic variants