Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats
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Emmanuel U. Akara
,
Victor C. Ude
Abstract
Objectives
Ocimum gratissimum L. is used in traditional medicine for the treatment of bacterial infections and anaemia. This study was designed to evaluate the effect of O. gratissimum leaf extract on phenylhydrazine (PHZ)-induced anaemia and toxicity in rats.
Methods
The experimental rats were divided into five groups (A–E) (n=6/sex/group). Each rat in groups B–E was intraperitoneally administered 50 mg/kg of PHZ for two consecutive days. Group A (normal control) did not receive any PHZ, group B (negative control), group C received orally 5 mg/kg ferrous sulphate whereas groups D and E received 200 and 400 mg/kg O. gratissimum leaf extract respectively, for 14 days.
Results
Red blood cell count, packed cell volume, haemoglobin concentration and high-density lipoprotein increased significantly (p<0.05) whereas low-density lipoprotein and very-low-density lipoprotein decreased in extract-treated groups when compared to the negative control. O. gratissimum (400 mg/kg extract) and standard drug (5 mg/kg ferrous sulphate) significantly (p<0.05) reduced the levels of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase.
Conclusions
The results of this study indicate that O. gratissimum leaf extract has a restorative effect on the phenylhydrazine-induced metabolic distortions in the blood, liver, and kidney, and therefore could be used therapeutically as an anti-anaemic tonic.
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Research funding: None declared.
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Author contributions: EAU and OE conceived the work, wrote the paper and did the statistical analysis. VCU wrote the paper and plot the graphs. CU, GE and EAU did the experiment and read the paper. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: This study received ethical approval (ABSU/REC/BMR/20) from Abia State University Ethical Committee.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Oxy+ (arthrospira) and its medicinal importance: an appraisal
- Original Articles
- In vitro inhibitory effects of glucosamine, chondroitin and diacerein on human hepatic CYP2D6
- Efficacy of Majoon-e-Seer Alvi Khan in dyslipidemia: a single blind randomized standard controlled clinical trial
- Prevalence of alcohol-drug interactions in community-dwelling older patients with polypharmacy
- Evaluation of the need for pharmacogenomics testing among physicians in the West Bank of Palestine
- Habitual khat chewing alters urinary inorganic profile in adult healthy males
- Trichostatin A sensitizes hepatoma cells to Taxol more than 5-Aza-dC and dexamethasone
- Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats
- Lactobacillus plantarum mitigates sexual-reproductive deficits by modulating insulin receptor expression in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice
- Consumption of Ashtanga Ghrita (clarified cow butter added with herb extracts) improves cognitive dysfunction induced by scopolamine in rats via regulation of acetylcholinesterase activity and oxidative stress
- Letter to the Editor
- Fatal thiopurine toxicity: pre-emptive testing of NUDT15 415C>T polymorphism may be life saving in South Asian population
Articles in the same Issue
- Frontmatter
- Review
- Oxy+ (arthrospira) and its medicinal importance: an appraisal
- Original Articles
- In vitro inhibitory effects of glucosamine, chondroitin and diacerein on human hepatic CYP2D6
- Efficacy of Majoon-e-Seer Alvi Khan in dyslipidemia: a single blind randomized standard controlled clinical trial
- Prevalence of alcohol-drug interactions in community-dwelling older patients with polypharmacy
- Evaluation of the need for pharmacogenomics testing among physicians in the West Bank of Palestine
- Habitual khat chewing alters urinary inorganic profile in adult healthy males
- Trichostatin A sensitizes hepatoma cells to Taxol more than 5-Aza-dC and dexamethasone
- Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats
- Lactobacillus plantarum mitigates sexual-reproductive deficits by modulating insulin receptor expression in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice
- Consumption of Ashtanga Ghrita (clarified cow butter added with herb extracts) improves cognitive dysfunction induced by scopolamine in rats via regulation of acetylcholinesterase activity and oxidative stress
- Letter to the Editor
- Fatal thiopurine toxicity: pre-emptive testing of NUDT15 415C>T polymorphism may be life saving in South Asian population
