Rutin ameliorates scopolamine-induced learning and memory impairments through enhancement of antioxidant defense system and cholinergic signaling
-
Ismail O. Ishola
,
Taiwo G. Olubodun-Obadun
Abstract
Objectives
The brain’s cholinergic system occupies a central role in normal cognition and age-related cognitive decline, including Alzheimer’s disease (AD). This study sought to investigate the role of antioxidant defense and cholinergic systems on rutin-induced antiamnesia in mice.
Methods
Rutin (1, 5, or 50 mg/kg, p.o.) or vehicle (10 ml/kg, p.o.) was administered for three consecutive days. One hour post-treatment on day 3, scopolamine (3 mg/kg, i.p) was given, 5 min post-scopolamine injection, open field, Y-maze, or Morris water maze (MWM) (five days consecutive training sessions) tasks was carried out. The mice were sacrificed on day 7 to assays for biomarkers of oxidative stress and cholinergic system.
Results
Scopolamine significantly reduced spontaneous alternation behavior in Y-maze and prolonged escape latency in MWM tasks when compared to vehicle-treated control indicative of working memory and spatial learning deficits. However, the pretreatment of mice with rutin (1, 5, or 50 mg/kg) prevented scopolamine-induced working memory and spatial learning impairments without affecting spontaneous locomotor activity. Scopolamine-induced nitrosative/oxidative stress and increased acetylcholinesterase activity in the prefrontal cortex and hippocampus were significantly attenuated by the pretreatment of mice with rutin.
Conclusions
rutin restored cognitive function in scopolamine-induced amnesia through enhancement of antioxidant defense and cholinergic systems.
Acknowledgments
We are grateful to Mr. M. C. Chijioke of the Department of Pharmacology, Therapeutics and Toxicology, CMUL for his technical assistance.
Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: The study was approved by the Health Research Ethics Committee of the College of Medicine, University of Lagos, Nigeria (CMUL/HREC/10/17/450) and is in accordance with the United States National Institute of Health Guidelines for Care and Use of Laboratory Animals in Biochemical Research (2002).
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Herbal approach for the management of C0VID-19: an overview
- Original Articles
- Clinically important drug–drug interactions in patients admitted to hospital with COVID-19: drug pairs, risk factors, and management
- Spinal and general anesthesia produces differential effects on oxidative stress and inflammatory cytokines in orthopedic patients
- Immunogenicity of antitumor necrosis factor therapy in patients with spondyloarthritis
- Biochip-based approach for comprehensive pharmacogenetic testing
- Clinical efficacy of Majoon Falasfa and Roghan-e-Surkh in post-stroke-disability: an open labeled, pre-post analysis
- Ascorbic acid improves extrapyramidal syndromes and corpus striatal degeneration induced by dopamine-2 receptor inhibition in Wistar rats
- Rutin ameliorates scopolamine-induced learning and memory impairments through enhancement of antioxidant defense system and cholinergic signaling
- Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats
Articles in the same Issue
- Frontmatter
- Review
- Herbal approach for the management of C0VID-19: an overview
- Original Articles
- Clinically important drug–drug interactions in patients admitted to hospital with COVID-19: drug pairs, risk factors, and management
- Spinal and general anesthesia produces differential effects on oxidative stress and inflammatory cytokines in orthopedic patients
- Immunogenicity of antitumor necrosis factor therapy in patients with spondyloarthritis
- Biochip-based approach for comprehensive pharmacogenetic testing
- Clinical efficacy of Majoon Falasfa and Roghan-e-Surkh in post-stroke-disability: an open labeled, pre-post analysis
- Ascorbic acid improves extrapyramidal syndromes and corpus striatal degeneration induced by dopamine-2 receptor inhibition in Wistar rats
- Rutin ameliorates scopolamine-induced learning and memory impairments through enhancement of antioxidant defense system and cholinergic signaling
- Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats
