Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?
-
Vasileios Kordinas
,
George Tsirpanlis
Abstract
Telomerase is involved in the elongation of telomeres. It remains active in very few types of cell in mature organisms. One such cell type is the lymphocytes. In this study, we investigated the activity and expression of telomerase in lymphocytes from renal failure patients and compared it to that for normal controls. Inflammation status was determined at the same time. The enzyme activity was measured using PCR-ELISA with peripheral blood mononuclear cells (PBMCs) from three groups: 53 healthy individuals, 50 patients with chronic kidney disease (CKD) and 50 dialysis patients. In the same cell populations, the expression of the reverse transcriptase of the human telomerase gene (hTERT) was measured via real-time PCR. The inflammationstatus of these individuals was determined by calculating the interleukin 6 (IL-6), IL-10, C-reactive protein (CRP) and tumor necrosis factor alpha (TNF-a) serum concentrations via ELISA. The lowest levels of telomerase activity were detected in CKD, and this group had the highest IL-6 and CRP values and the lowest hTERT expression. The dialysis group showed significant differences in comparison to the normal subjects and to the CKD patients. Further studies are warranted in order to explore the way inflammation influences telomerase activity and hTERT expression.
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© University of Wrocław, Poland
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Articles in the same Issue
- Biochemical evidence for Ca2+-independent functional activation of hPLSCR1 at low pH
- Catabolism of chondroitin sulfate
- The effect of hypoxia on PGE2-stimulated cAMP generation in HMEC-1
- Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?
- The beta-actin gene promoter of rohu carp (Labeo rohita) drives reporter gene expressions in transgenic rohu and various cell lines, including spermatogonial stem cells
- Molecular machines – a new dimension of biological sciences
- Therapeutic potential of PACAP for neurodegenerative diseases
- A newly isolated yeast as an expression host for recombinant lipase
- Identification of shorter length lamin A protein in mouse ear cartilage tissue
- Relationship among IL-6, LDL cholesterol and lipid peroxidation
- Study strategies for long non-coding RNAs and their roles in regulating gene expression
