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Antioxidant effect of a fermented powder of Lady Joy bean in primary rat hepatocytes

  • Margherita La Marca , Laura Pucci , Roberto Bollini , Rossella Russo , Francesca Sparvoli , Morena Gabriele and Vincenzo Longo EMAIL logo
Published/Copyright: March 25, 2015
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Cellular and Molecular Biology Letters
From the journal Cellular and Molecular Biology Letters Volume 20 Issue 1

Abstract

The role and beneficial effects of plant and food extracts against various diseases induced by oxidative stress have received much attention in recent years. Legumes are rich in bioactive compounds, and some studies suggest a correlation between their consumption and a reduced incidence of diseases. Primary cultures of rat hepatocytes were used to investigate whether and how an extract obtained from a fermented powder of bean named Lady Joy (Phaseolus vulgaris L.) is able to regulate antioxidant and detoxifying enzymes through the NRF2 pathway, inhibit NF-kB activation, and reduce H2O2-induced endoplasmic reticulum (ER) stress. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by Lady Joy treatment. Western blot showed that Nrf2 was activated by Lady Joy treatment. Also, cells treated with this fermented bean were partially protected against NF-kB activation resulting from H2O2 stress. As a link between oxidative stress and ER dysfunction is hypothesized, we verified whether Lady Joy was able to protect cells from H2O2-induced ER stress, by studying the response of the proteins CHOP, BiP and caspase 12. The results of this study show that Lady Joy can induce the Nrf2 pathway, inhibit NF-kB, and protect ER from stress induced by H2O2.

Keywords : Fermented bean; Antioxidants; Anti-inflammatory; Oxidative stress; Hepatocytes; NRF2; NF-kB; Antioxidant enzymes

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Received: 2014-8-5
Accepted: 2015-1-26
Published Online: 2015-3-25
Published in Print: 2015-3-1

© 2015 University of Wrocław, Poland

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https://doi.org/10.1515/cmble-2015-0007
Keywords for this article
Fermented bean; Antioxidants; Anti-inflammatory; Oxidative stress; Hepatocytes; NRF2; NF-kB; Antioxidant enzymes

Articles in the same Issue

  1. Identification of drought-induced transcription factors in Sorghum bicolor using GO term semantic similarity
  2. Evaluation of the potential of alkylresorcinols as superoxide anion scavengers and sox-regulon modulators using nitroblue tetrazolium and bioluminescent cell-based assays
  3. Cyclic phosphatidic acid induces G0/G1 arrest, inhibits AKT phosphorylation, and downregulates cyclin D1 expression in colorectal cancer cells
  4. Polymorphism of the APEX nuclease 1 gene in keratoconus and Fuchs endothelial corneal dystrophy
  5. FE65: Roles beyond amyloid precursor protein processing
  6. Single nucleotide polymorphisms in the STAT3 gene influence AITD susceptibility, thyroid autoantibody levels, and IL6 and IL17 secretion
  7. Antioxidant effect of a fermented powder of Lady Joy bean in primary rat hepatocytes
  8. Oncogene-dependent survival of highly transformed cancer cells under conditions of extreme centrifugal force – implications for studies on extracellular vesicles
  9. Changes in cell death of peripheral blood lymphocytes isolated from children with acute lymphoblastic leukemia upon stimulation with 7 Hz, 30 mT pulsed electromagnetic field
  10. Flow cytometric analysis of apoptosis in cryoconserved chicken primordial germ cells
  11. GGeneration of an efficient artificial promoter of bovine skeletal muscle alpha-actin gene (ACTA1) through addition of cis-acting element
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