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Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum
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Evi Debruyne
Published/Copyright:
August 23, 2011
Abstract
Background: Gc-globulin is a polymorphic protein with three phenotypes: Gc 1-1, Gc 2-1 and Gc 2-2. Deglycosylation of Gc-globulin results in a Gc-macrophage activating factor (Gc-MAF). This study investigated the potential of MAF as a tumour marker and the influence of Gcphenotypes on serum MAF-concentrations.
Methods: Gc-phenotype of 98 healthy individuals and 60 cancer patients was determined. MAF-levels of healthy individuals and cancer patients were analysed according to their Gc-phenotype using a Helix pomatia agglutinin-based ELISA. ROC curves analysed the efficiency of MAF as a tumour marker.
Results: MAF-levels between controls and patients were significantly different (p<0.001). No phenotypic differences were found in the patients. In comparison with the controls, MAF-values were significantly lower in cancer patients carrying Gc 1-1 (p<0.01) and Gc 2-1 (p<0.001). No difference was observed in Gc 2-2 phenotype. Diagnostic accuracy of MAF as a tumour marker also demonstrated pronounced differences between Gc-phenotypes.
Conclusions: Gc-phenotype is a confounding factor when interpreting MAF-data. The value of MAF as a tumour marker varies according to phenotype. Future studies on MAF will have to consider the effect of Gc-phenotype.
Keywords: Gc-globulin; Gc-MAF; Helix pomatia agglutinin; lectin-based ELISA; phenotype; tumour marker
Received: 2011-5-23
Accepted: 2011-7-25
Published Online: 2011-08-23
Published in Print: 2011-11-01
©2011 by Walter de Gruyter Berlin Boston
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Keywords for this article
Gc-globulin;
Gc-MAF;
Helix pomatia agglutinin;
lectin-based ELISA;
phenotype;
tumour marker
Articles in the same Issue
- Editorial
- Thank you, indeed!
- Reviews
- Glomerular filtration rate estimation: performance of serum cystatin C-based prediction equations
- Oxidative stress and diabetes mellitus
- Human papilloma virus (HPV) molecular diagnostics
- Opinion Papers
- Urine immunofixation electrophoresis remains important and is complementary to serum free light chain
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