Comparing Different Methods for Homocysteine Determination
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Bruno Zappacosta
Abstract
Slightly elevated values of homocysteine are commonly associated with thromboembolic diseases, while high values can be found in patients with congenital metabolic defects or nutritional problems. The clinical use of homocysteine as an independent marker of cardiovascular disease was limited in the past by technical problems with its measurement, the instrumentation (HPLC, radioenzymatic assays, gas chromatography-mass spectrometry, etc.) and the necessary skills required. Commercially available immunoassays now permit a simpler and more rapid measurement of homocysteine, that is more suitable for routine clinical laboratories; in this paper we analyze the results obtained by using three fully automated methods for homocysteine determination (Abbott IMx immunoassay, Abbott AxSYM immunoassay and Immulite 2000 homocysteine immunoassay) and their correlation with the widely used HPLC method.
The results clearly indicate that all three automated immunochemical methods correlate well with the HPLC method (slope 0.97–1.03; intercept 0.95–1.91 with a recovery above 95% for all three methods).
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Sickle Cell Disease – Pathophysiology, Clinical and Diagnostic Implications
- Family Studies: Their Role in the Evaluation of Genetic Cardiovascular Risk Factors
- Polymorphisms in the Lipopolysaccharide-Binding Protein and Bactericidal/Permeability-Increasing Protein in Patients with Myocardial Infarction
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- Hyperhomocysteinemia Is Related to a Decreased Blood Level of Vitamin B12 in the Second and Third Trimester of Normal Pregnancy
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