Stability of direct renin concentration and plasma renin activity in EDTA whole blood and plasma at ambient and refrigerated temperatures from 0 to 72 hours
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Sophie Hepburn
,
Coral Munday
Abstract
Objectives
The aim of this study was to determine the appropriate transport and storage conditions for blood taken for direct renin concentration and plasma renin activity measurement, and whether cryoactivation of prorenin is seen at time points relevant to clinical practice.
Methods
Blood was extracted from n=10 volunteers into K2-EDTA tubes. Stability of renin was assessed in whole blood stored at room temperature (15–25 °C) and in the refrigerator (2–8 °C) at 0 h, 8 h, and 24 h. The stability of renin in plasma was determined under the same conditions at 0 h, 24 h and 72 h.
Results
Stability of plasma renin activity and direct renin concentration in whole blood stored at room temperature was found to be acceptable for up to 24 h. At refrigerated temperature, whole blood stability was acceptable for measurement of direct renin concentration up to 8 h and plasma renin activity up to 24 h. In contrast, plasma renin activity was not stable in plasma stored at either room or refrigerated temperatures up to 24 h; however, direct renin concentration had acceptable stability in plasma stored at room temperature for up to 24 h, but stability was unacceptable at refrigerated temperatures.
Conclusions
Samples collected for plasma renin activity and direct renin concentration should be transported as whole blood to optimise stability. After sample processing, plasma can be kept at room temperature for up to 24 h for direct renin concentration, however, for determination of plasma renin activity separated plasma should be analysed or frozen as soon as possible.
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Research funding: None declared.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from all individuals included in this study.
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Ethical approval: Not applicable.
References
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorials
- Clinical Chemistry and Laboratory Medicine: enjoying the present and assessing the future
- Rethinking internal quality control: the time is now
- Review
- Multi-omics analysis from archival neonatal dried blood spots: limitations and opportunities
- Opinion Papers
- ‘Penelope test’: a practical instrument for checking appropriateness of laboratory tests
- Interference by macroprolactin in assays for prolactin: will the In Vitro Diagnostics Regulation lead to a solution at last?
- EFLM Paper
- Efficiency, efficacy and subjective user satisfaction of alternative laboratory report formats. An investigation on behalf of the Working Group for Postanalytical Phase (WG-POST), of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
- General Clinical Chemistry and Laboratory Medicine
- Cross-reactivity in assays for prolactin and optimum screening policy for macroprolactinaemia
- Repository of intra- and inter-run variations of quantitative autoantibody assays: a European multicenter study
- Stability of direct renin concentration and plasma renin activity in EDTA whole blood and plasma at ambient and refrigerated temperatures from 0 to 72 hours
- Comparison of four different immunoassays and a rapid isotope-dilution liquid chromatography-tandem mass spectrometry assay for serum folate
- Analytical quality specifications in semen analysis according to the state of the current methodologies
- Reference Values and Biological Variations
- Short-term biological variation study of plasma hemophilia and thrombophilia parameters in a population of apparently healthy Caucasian adults
- First morning voided urinary gonadotropins in children: verification of method performance and establishment of reference intervals
- Derivation of sex and age-specific reference intervals for clinical chemistry analytes in healthy Ghanaian adults
- Cancer Diagnostics
- Serum free light chain analysis: persisting limitations with new kids on the block
- Cardiovascular Diseases
- Age partitioned and continuous upper reference limits for Ortho VITROS High Sensitivity Troponin I in a healthy paediatric cohort
- The predictive value of hemoglobin to creatinine ratio for contrast-induced nephropathy in percutaneous coronary interventions
- Infectious Diseases
- Health technology assessment to employ COVID-19 serological tests as companion diagnostics in the vaccination campaign against SARS-CoV-2
- Evaluation of a laboratory-based high-throughput SARS-CoV-2 antigen assay
- Presepsin levels in neonatal cord blood are not influenced by maternal SARS-CoV-2 infection
- Letters to the Editors
- How to evaluate fixed clinical QC limits vs. risk-based SQC strategies
- Reply to Westgard et al.: ‘Keep your eyes wide … as the present now will later be past’*
- Platelet phagocytosis by monocytes
- Early detection of Candida parapsilosis in peripheral blood as a result of a peripheral blood smear performed after cytographic changes on the Beckman Coulter UniCel DxH 800 hematology
- Pseudo-erythroblastosis on Sysmex XN hematology analyzers: a clue to Candida sepsis. Case report and literature review
- Covert poisoning with difenacoum: diagnosis and follow-up difficulties
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