Streamlined three step total vitamin C analysis by HILIC-UV for laboratory testing
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Michael Fitzpatrick
,
Paul Bonnitcha
Abstract
Objectives
In the clinical setting, the analysis and quantification of vitamin C (ascorbic acid) poses several challenges including analyte instability and poor retention by reverse phase HPLC systems. In this article we describe a rapid hydrophilic interaction chromatography ultraviolet method for the measurement of total vitamin C in plasma which overcomes these issues.
Methods
Ascorbic acid and the internal standard were separated under isocratic conditions using a Waters BEH-Amide column and a mobile phase containing 0.005 M potassium phosphate in 80% acetonitrile.
Results
The proposed method was validated and showed good precision (coefficient of variation <5%), accuracy (>99%), and analyte stability after extraction (>24 h).
Conclusions
The simple sample preparation allows full automation and rapid analytical run times of the assay and is therefore suitable for a high-throughput clinical chromatography laboratory.
Acknowledgments
The authors would like to acknowledge the RCPAQAP for use of their proficiency testing data. The authors would also like to acknowledge the support of the staff and Professor David Sullivan of the New South Wales Health Pathology, Chemical Pathology Department at Royal Prince Alfred Hospital.
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Research funding: None declared.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: Not applicable.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Editorials
- Optimizing effectiveness of COVID-19 vaccination: will laboratory stewardship play a role?
- Mathematical recalibration of total bile acids: comparing the incomparable?
- Reviews
- Could metabolomics drive the fate of COVID-19 pandemic? A narrative review on lights and shadows
- Low free-T3 serum levels and prognosis of COVID-19: systematic review and meta-analysis
- Opinion Paper
- Pancreatic lipase: why laboratory community does not take enough care of this clinically important test?
- General Clinical Chemistry and Laboratory Medicine
- The BACH project protocol: an international multicentre total Bile Acid Comparison and Harmonisation project and sub-study of the TURRIFIC randomised trial
- Comparability of 11 different equations for estimating LDL cholesterol on different analysers
- Streamlined three step total vitamin C analysis by HILIC-UV for laboratory testing
- Hematology and Coagulation
- Anti-phosphatidyl-serine/prothrombin antibodies (aPS/PT) in isolated lupus anticoagulant (LA): is their presence linked to dual test positivity?
- Cancer Diagnostics
- Consideration should be given to smoking, endometriosis, renal function (eGFR) and age when interpreting CA125 and HE4 in ovarian tumor diagnostics
- Monitoring the M-protein of multiple myeloma patients treated with a combination of monoclonal antibodies: the laboratory solution to eliminate interference
- Cardiovascular Diseases
- Identification of macrotroponin T: findings from a case report and non-reproducible troponin T results
- Diabetes
- Impact of optimizing pre-analytical phase on the diagnosis of gestational diabetes and related outcomes
- Infectious Diseases
- Daily monitoring of viral load measured as SARS-CoV-2 antigen and RNA in blood, IL-6, CRP and complement C3d predicts outcome in patients hospitalized with COVID-19
- Alternative detection of SARS-CoV-2 RNA by a new assay based on mass spectrometry
- Performance evaluation of an automated SARS-CoV-2 Ag test for the diagnosis of COVID-19 infection on nasopharyngeal swabs
- Predicting the protective humoral response to a SARS-CoV-2 mRNA vaccine
- Quantitative serological evaluation as a valuable tool in the COVID-19 vaccination campaign
- Letters to the Editors
- Effects of various pre-analytical conditions on blood-based biomarkers of Alzheimer’s disease
- Stability of lactate in venous blood gas and sodium fluoride-potassium oxalate plasma: a 6-year retrospective database analysis
- Validation of the Fujirebio Lumipulse G1200 aldosterone assay for measurements during adrenal venous sampling for primary hyperaldosteronism
- The new Roche Elecsys TSH assay conforms with current IFCC C-STFT standards
- Development of an inverse-PCR approach for characterization of the major BCR-ABL1 breakpoint sequences on genomic DNA: proof of concept
- Antiphospholipid antibodies quantification using ALBIA technology: how to define an optimal cutoff?
- Simultaneous occurrence of EDTA-dependent lymphoagglutination and agglutination of myeloid cells in a patient with chronic myelomonocytic leukemia
- NT-proBNP use in old patients and the impact of the selected threshold value: a big data analysis
- Congress Abstracts
- 53rd National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC – Laboratory Medicine)
Artikel in diesem Heft
- Frontmatter
- Editorials
- Optimizing effectiveness of COVID-19 vaccination: will laboratory stewardship play a role?
- Mathematical recalibration of total bile acids: comparing the incomparable?
- Reviews
- Could metabolomics drive the fate of COVID-19 pandemic? A narrative review on lights and shadows
- Low free-T3 serum levels and prognosis of COVID-19: systematic review and meta-analysis
- Opinion Paper
- Pancreatic lipase: why laboratory community does not take enough care of this clinically important test?
- General Clinical Chemistry and Laboratory Medicine
- The BACH project protocol: an international multicentre total Bile Acid Comparison and Harmonisation project and sub-study of the TURRIFIC randomised trial
- Comparability of 11 different equations for estimating LDL cholesterol on different analysers
- Streamlined three step total vitamin C analysis by HILIC-UV for laboratory testing
- Hematology and Coagulation
- Anti-phosphatidyl-serine/prothrombin antibodies (aPS/PT) in isolated lupus anticoagulant (LA): is their presence linked to dual test positivity?
- Cancer Diagnostics
- Consideration should be given to smoking, endometriosis, renal function (eGFR) and age when interpreting CA125 and HE4 in ovarian tumor diagnostics
- Monitoring the M-protein of multiple myeloma patients treated with a combination of monoclonal antibodies: the laboratory solution to eliminate interference
- Cardiovascular Diseases
- Identification of macrotroponin T: findings from a case report and non-reproducible troponin T results
- Diabetes
- Impact of optimizing pre-analytical phase on the diagnosis of gestational diabetes and related outcomes
- Infectious Diseases
- Daily monitoring of viral load measured as SARS-CoV-2 antigen and RNA in blood, IL-6, CRP and complement C3d predicts outcome in patients hospitalized with COVID-19
- Alternative detection of SARS-CoV-2 RNA by a new assay based on mass spectrometry
- Performance evaluation of an automated SARS-CoV-2 Ag test for the diagnosis of COVID-19 infection on nasopharyngeal swabs
- Predicting the protective humoral response to a SARS-CoV-2 mRNA vaccine
- Quantitative serological evaluation as a valuable tool in the COVID-19 vaccination campaign
- Letters to the Editors
- Effects of various pre-analytical conditions on blood-based biomarkers of Alzheimer’s disease
- Stability of lactate in venous blood gas and sodium fluoride-potassium oxalate plasma: a 6-year retrospective database analysis
- Validation of the Fujirebio Lumipulse G1200 aldosterone assay for measurements during adrenal venous sampling for primary hyperaldosteronism
- The new Roche Elecsys TSH assay conforms with current IFCC C-STFT standards
- Development of an inverse-PCR approach for characterization of the major BCR-ABL1 breakpoint sequences on genomic DNA: proof of concept
- Antiphospholipid antibodies quantification using ALBIA technology: how to define an optimal cutoff?
- Simultaneous occurrence of EDTA-dependent lymphoagglutination and agglutination of myeloid cells in a patient with chronic myelomonocytic leukemia
- NT-proBNP use in old patients and the impact of the selected threshold value: a big data analysis
- Congress Abstracts
- 53rd National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC – Laboratory Medicine)
