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Relationship between polymorphisms in the CRP, LEP and LEPR genes and high sensitivity C-reactive protein levels in Spanish children

  • Pilar Navarro , Olaya de Dios , Teresa Gavela-Pérez , Leandro Soriano-Guillen and Carmen Garcés EMAIL logo
Published/Copyright: March 28, 2017
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 55 Issue 11

Abstract

Background:

We investigated the association of single nucleotide polymorphisms (SNPs) in the C-reactive protein (CRP), leptin (LEP) and leptin receptor (LEPR) genes with high sensitivity CRP (hs-CRP) levels in two independent cohorts of healthy Spanish children.

Methods:

We measured hs-CRP levels in 646 6–8-year-old and 707 12–16-year-old children using a high-sensitivity C-Reactive Protein ELISA kit. Four SNPs in the CRP gene (rs1205, rs1130864, rs2794521 and rs1800947), one SNP in the LEP gene (rs7799039) and two SNPs in the LEPR (rs1137100 and rs1137101) gene were determined by TaqMan® allelic discrimination assays.

Results:

The four CRP SNPs studied were significantly (p<0.05) associated with hs-CRP levels in both cohorts. Furthermore, two common CRP haplotypes (constructed using the SNPs in order: rs1205, rs1130864, rs1800947, rs2794521) ACGA and GCGG were associated with significantly lower CRP levels (p<0.05) at both ages. The LEPR SNPs rs1137100 (K109R) and rs1137101 (Q223R), and LEP SNP rs7799039 (G2548A) were also associated to hs-CRP levels (p<0.05) in both cohorts.

Conclusions:

hs-CRP levels in healthy Spanish children, besides being associated to common polymorphisms in the CRP gene, are associated to polymorphisms in the LEP and LEPR genes, which suggests that other loci, in addition the CRP gene, may have a role determining CRP levels in children.

Keywords: children; CRP polymorphisms; hs-CRP levels; LEP polymorphisms
Corresponding author: Dr. Carmen Garcés, Lipid Research Laboratory, IIS-Fundación Jiménez Díaz, Avda. Reyes Católicos, 2. 28040 Madrid, Spain, Phone: +34-91-5432880

Acknowledgments

This article is dedicated to the late Prof. Manuel de Oya as the warmest homage to his memory. Prof. de Oya designed the Four Province Study.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was supported by a grant from the Fondo de Investigación Sanitaria /FEDER (FIS14/00344) and Biobank grant FEDER RD09/0076/00101.

  3. Employment or leadership: Pilar Navarro and Olaya de Dios are fellows of the Conchita Rábago Foundation.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2017-2-16
Accepted: 2017-2-19
Published Online: 2017-3-28
Published in Print: 2017-10-26

©2017 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2017-0134
Keywords for this article
children; CRP polymorphisms; hs-CRP levels; LEP polymorphisms

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. High-sensitivity assays for cardiac troponins – continued
  4. Reviews
  5. The 99th percentile of reference population for cTnI and cTnT assay: methodology, pathophysiology and clinical implications
  6. Vitamin B1 in critically ill patients: needs and challenges
  7. Opinion Papers
  8. Point
  9. High-sensitivity cardiac troponin: do think twice, it’s not all right
  10. Counterpoint
  11. Terminology of cardiac troponin assays and data censoring
  12. Establishing consensus-based, assay-specific 99th percentile upper reference limits to facilitate proper utilization of cardiac troponin measurements
  13. Fast track protocols using highly sensitive troponin assays for ruling out and ruling in non-ST elevation acute coronary syndrome
  14. Genetics and Molecular Diagnostics
  15. Relationship between polymorphisms in the CRP, LEP and LEPR genes and high sensitivity C-reactive protein levels in Spanish children
  16. General Clinical Chemistry and Laboratory Medicine
  17. An approach for estimating measurement uncertainty in medical laboratories using data from long-term quality control and external quality assessment schemes
  18. Selecting multi-rule quality control procedures based on patient risk
  19. Moving sum of number of positive patient result as a quality control tool
  20. Multidisciplinary training activities for decreasing preanalytical mistakes in samples from primary care
  21. Harmonization protocols for TSH immunoassays: a multicenter study in Italy
  22. Circulating free light chain measurement in the diagnosis, prognostic assessment and evaluation of response of AL amyloidosis: comparison of Freelite and N latex FLC assays
  23. Serum prolactin revisited: parametric reference intervals and cross platform evaluation of polyethylene glycol precipitation-based methods for discrimination between hyperprolactinemia and macroprolactinemia
  24. Temporal and regional variability in the request of vitamin D from general practitioners in Spain
  25. Fibrinogen determination according to Clauss: commutability assessment of International and commercial standards and quality control samples
  26. Reference Values and Biological Variations
  27. S100B maternal blood levels are gestational age- and gender-dependent in healthy pregnancies
  28. Gestational age-specific reference intervals for serum thyroid hormone levels in a multi-ethnic population
  29. Cancer Diagnostics
  30. Serum complexed and free prostate-specific antigen (PSA) for the diagnosis of the polycystic ovarian syndrome (PCOS)
  31. Cardiovascular Diseases
  32. A new immunochemistry platform for a guideline-compliant cardiac troponin T assay at the point of care: proof of principle
  33. Diabetes
  34. Adiponectin and leptin as first trimester markers for gestational diabetes mellitus: a cohort study
  35. Letters to the Editor
  36. Can we still trust hemoglobin A1c in all situations?
  37. Automated detection of unstable hemoglobin variants by Sysmex XE-Series analyzers
  38. Mean hemoglobin concentrations in fasting venous and non-fasting capillary blood of Cambodian women using a hemoglobinometer and an automated hematology analyzer
  39. Evaluation of a POCT device for C-reactive protein, hematocrit and leukocyte differential
  40. New perspectives on existing data in comparative measurements: a simple extension of the regression analysis
  41. Evaluation of a human anti-mouse antibody rapid test for patients requiring radio-immunodiagnostic
  42. The cortisol-CBG ratio affects cortisol immunoassay bias at elevated CBG concentrations
  43. Prognostic value of total antioxidant capacity to predict functional outcome in traumatic brain injury patients
  44. Use of IFCC guidelines to verify acetylcholinesterase reference interval in adults determined with ChE check mobile testing system
  45. Congress Abstracts
  46. 49th National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC – Laboratory Medicine)
  47. Congress of Clinical Chemistry and Laboratory Medicine
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