Comparison of ex vivo stability of copeptin and vasopressin
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Judith E. Heida
Abstract
Background:
Copeptin, part of the vasopressin precursor, is increasingly used as marker for vasopressin and is claimed to have better ex vivo stability. However, no study has directly compared the ex vivo stability of copeptin and vasopressin.
Methods:
Blood of ten healthy volunteers was collected in EDTA tubes. Next, we studied the effect of various pre-analytical conditions on measured vasopressin and copeptin levels: centrifugation speed, short-term storage temperature and differences between whole blood and plasma, long-term storage temperature and repeated freezing and thawing. The acceptable change limit (ACL), indicating the maximal percentage change that can be explained by assay variability, was used as cut-off to determine changes in vasopressin and copeptin.
Results:
The ACL was 25% for vasopressin and 19% for copeptin. Higher centrifugation speed resulted in lower vasopressin levels, whereas copeptin concentration was unaffected. In whole blood, vasopressin was stable up to 2 h at 25°C and 6 h at 4°C. In plasma, vasopressin was stable up to 6 h at 25°C and 24 h at 4°C. In contrast, copeptin was stable in whole blood and plasma for at least 24h at both temperatures. At –20°C, vasopressin was stable up to 1 month and copeptin for at least 4 months. Both vasopressin and copeptin were stable after 4 months when stored at –80°C and –150°C. Vasopressin concentration decreased after four freeze-thaw cycles, whereas copeptin concentration was unaffected.
Conclusion:
Vasopressin levels were considerably affected by pre-analytical conditions, while copeptin levels were stable. Therefore, a strict sample handling protocol for measurement of vasopressin is recommended.
Acknowledgments
The laboratory staff of the Department of Clinical Chemistry (IJsselland Hospital, Capelle aan den IJssel, The Netherlands) is acknowledged for their assistance in data collection and analysis and especially Ton Kerdel for his skillful measurement of vasopressin.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Dutch Kidney Foundation, (Grant/Award Number: 16OKK04).
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Supplemental Material:
The online version of this article (DOI: https://doi.org/10.1515/cclm-2016-0559) offers supplementary material, available to authorized users.
©2017 Walter de Gruyter GmbH, Berlin/Boston
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