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Physiologic changes of urinary proteome by caffeine and excessive water intake

  • Paleerath Peerapen , Nardtaya Ausakunpipat , Suchitra Sutthimethakorn , Siripat Aluksanasuwan , Arada Vinaiphat and Visith Thongboonkerd EMAIL logo
Published/Copyright: December 17, 2016
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 55 Issue 7

Abstract

Background:

Diurnal variations and physiologic changes of urinary proteome have been suggested in the urinary proteomics field. However, no clear evidence has been demonstrated. The present study thus aimed to define changes in urinary proteome by physiological stimuli, i.e. caffeine intake and excessive water drinking, both of which cause physiologic diuresis.

Methods:

Urine samples were collected from 30 healthy individuals under three different conditions: (i) morning void as the control; (ii) after drinking a cup of coffee; and (iii) after drinking 1 L of water within 20 min. Thereafter, differentially excreted proteins were analyzed by 2-DE proteomics approach and validated by Western blotting and ELISA.

Results:

Spot matching, quantitative intensity analysis, and ANOVA followed by Tukey’s post-hoc multiple comparisons and the Bonferroni correction revealed significant differences in levels of five protein spots among three different conditions. These proteins were identified by quadrupole time-of-flight mass spectrometry (Q-TOF MS) and/or MS/MS analyses as kininogen 1 isoform 3, β-actin, prostaglandin D synthase (PGDS), fibrinogen α-chain and immunoglobulin light chain. Among these, the decreased level of immunoglobulin was successfully validated by Western blotting and ELISA.

Conclusions:

These data indicated that caffeine intake and excessive water drinking could affect urinary excretion of some proteins and may affect urinary proteome analysis.

Keywords: actin; caffeine; fibrinogen; immunoglobulin; kinonogen; prostaglandin D synthase
Corresponding author: Prof. Visith Thongboonkerd, Head of Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, 6th Floor – SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand; and Center for Research in Complex Systems Science, Mahidol University, Bangkok, Thailand, Phone: +66-2-4192850, E-mail:

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. PP, NA, SS, SA, AV and VT designed research; PP, NA, SS, SA and AV performed experiments; PP, NA, SS, SA, AV and VT analyzed data; PP, SS, SA, AV and VT wrote the manuscript; all authors reviewed and approved the manuscript.

  2. Research funding: This study was supported by Mahidol University research grant, Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative, and the Thailand Research Fund (RTA5680004 and IRG5980006). VT is supported by “Chalermphrakiat” and “Research Staff” Grants from Faculty of Medicine Siriraj Hospital, whereas SA is supported by the Royal Golden Jubilee PhD Program of The Thailand Research Fund.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Supplemental Material:

The online version of this article (DOI: https://doi.org/10.1515/cclm-2016-0464) offers supplementary material, available to authorized users.

Received: 2016-5-30
Accepted: 2016-10-12
Published Online: 2016-12-17
Published in Print: 2017-6-27

©2017 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2016-0464
Keywords for this article
actin; caffeine; fibrinogen; immunoglobulin; kinonogen; prostaglandin D synthase

Articles in the same Issue

  1. Frontmatter
  2. Editorials
  3. Opportunities and drawbacks of nonstandard body fluid analysis
  4. How I first met Dr. Morton K. Schwartz
  5. Reviews
  6. Measurement of thyroglobulin, calcitonin, and PTH in FNA washout fluids
  7. Quality control materials for pharmacogenomic testing in the clinic
  8. Modulating thrombotic diathesis in hereditary thrombophilia and antiphospholipid antibody syndrome: a role for circulating microparticles?
  9. Opinion Papers
  10. Advances in laboratory diagnosis of hereditary spherocytosis
  11. Analytical performance specifications for external quality assessment – definitions and descriptions
  12. Genetics and Molecular Diagnostics
  13. Differences between quantification of genotype 3 hepatitis C virus RNA by Versions 1.0 and 2.0 of the COBAS AmpliPrep/COBAS TaqMan HCV Test
  14. General Clinical Chemistry and Laboratory Medicine
  15. Estimating the intra- and inter-individual imprecision of manual pipetting
  16. Effect of multiple freeze-thaw cycles on selected biochemical serum components
  17. The effect of storage temperature fluctuations on the stability of biochemical analytes in blood serum
  18. Comparison of ex vivo stability of copeptin and vasopressin
  19. Physiologic changes of urinary proteome by caffeine and excessive water intake
  20. Assessment of autoantibodies to interferon-ω in patients with autoimmune polyendocrine syndrome type 1: using a new immunoprecipitation assay
  21. Reference Values and Biological Variations
  22. Within-day biological variation and hour-to-hour reference change values for hematological parameters
  23. Relationship between anti-Müllerian hormone and antral follicle count across the menstrual cycle using the Beckman Coulter Access assay in comparison with Gen II manual assay
  24. Cardiovascular Diseases
  25. Low-grade inflammation and tryptophan-kynurenine pathway activation are associated with adverse cardiac remodeling in primary hyperparathyroidism: the EPATH trial
  26. Infectious Diseases
  27. Comparison between procalcitonin and C-reactive protein in predicting bacteremias and confounding factors: a case-control study
  28. Monitoring of procalcitonin but not interleukin-6 is useful for the early prediction of anastomotic leakage after colorectal surgery
  29. Activation of the tryptophan/serotonin pathway is associated with severity and predicts outcomes in pneumonia: results of a long-term cohort study
  30. Letters to the Editor
  31. Incidental findings of monoclonal proteins from carbohydrate-deficient transferrin analysis using capillary electrophoresis
  32. IgD-λ myeloma with extensive free light-chain excretion: a diagnostic pitfall in the identification of monoclonal gammopathies
  33. 25-Hydroxyvitamin D threshold values should be age-specific
  34. Effect of dabigatran treatment at therapeutic levels on point-of-care international normalized ratio (INR)
  35. Alkaline phosphatase activity – pH impact on the measurement result
  36. Cyst hydatid and cancer: the myth continues
  37. Role of activated platelets in severe acne scarring and adaptive immunity activation
  38. Towards a random-access LC-MS/MS model for busulfan analysis
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