Activity and expression of dipeptidyl peptidase IV on peripheral blood mononuclear cells in patients with early steroid and disease modifying antirheumatic drugs naïve rheumatoid arthritis
-
Milica Grujic
,
Ivana Z. Matic
Abstract
Background:
Dipeptidyl peptidase IV (DPPIV/CD26) plays an important role in T cell activation and immune regulation, however the role of this enzyme in early rheumatoid arthritis (eRA) has not been clearly defined. The aim of this study was to determine the serum activity of DPPIV, its expression on peripheral blood mononuclear cells (PBMC) and to examine possible correlations with disease activity (DAS28) in untreated patients with eRA.
Methods:
The study included 50 patients newly diagnosed with RA, who had not received any corticosteroid or disease modifying antirheumatic drugs (DMARD) therapy and whose conventional radiographs of hands and feet showed no structural damage. The control group consisted of 40 healthy volunteers. Also, 30 patients with chronic RA (cRA) were examined. The serum activity of DPPIV was determined by the direct photometric method, while expression of CD26 on PBMC was determined using flow cytometry.
Results:
Decreased DPPIV serum activity was detected in patients with eRA and cRA compared to the control group (p=0.024, p<0.0001, respectively). Although, the percentage of overall CD26+ white blood cells (WBC) was significantly decreased in eRA patients (p<0.001), the percentage of CD26+ lymphocytes and monocytes and mean fluorescence intensity of CD26 on these cells in eRA patients showed no significant difference compared to healthy volunteers. DAS28 showed no significant correlation with CD26 expression or DPPIV serum activity, but a significant inverse correlation between the duration of symptoms and DPPIV serum activity was observed.
Conclusions:
Our results show that a decrease in DPPIV serum activity, but not CD26 expression, is present in an early stage of rheumatoid arthritis.
Acknowledgments
Also, the authors would like to thank Tatjana Petrović for her excellent technical assistance and Zorica Ivković for editing the text.
Author contributions:All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: The authors are grateful to the Ministry of Education, Science and Technological Development of the Republic of Serbia for the financial support (Project 175011).
Employment or leadership:None declared.
Honorarium: None declared.
Competing interests:The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Editorial
- Describing children’s changes using clinical chemistry analytes
- Review
- Assessment of vitamin D status – a changing landscape
- Opinion Papers
- Order of blood draw: Opinion Paper by the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE)
- The principles of Health Technology Assessment in laboratory medicine
- Genetics and Molecular Diagnostics
- Functional polymorphism of lncRNA MALAT1 contributes to pulmonary arterial hypertension susceptibility in Chinese people
- General Clinical Chemistry and Laboratory Medicine
- Influence of pneumatic tube system transport on routinely assessed and spectrophotometric cerebrospinal fluid parameters
- Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples
- Evaluation of the first fully automated immunoassay method for the measurement of stimulating TSH receptor autoantibodies in Graves’ disease
- Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children
- Activity and expression of dipeptidyl peptidase IV on peripheral blood mononuclear cells in patients with early steroid and disease modifying antirheumatic drugs naïve rheumatoid arthritis
- Hematology and Coagulation
- Neutrophil CD64 Index as a superior biomarker for early diagnosis of infection in febrile patients in the hematology department
- Dilute Russell’s viper venom time reagents in lupus anticoagulant testing: a well-considered choice
- Reference Values and Biological Variations
- Pediatric reference intervals for alkaline phosphatase
- Cancer Diagnostics
- Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target
- CDH1 (E-cadherin) expression independently affects clinical outcome in acute myeloid leukemia with normal cytogenetics
- Cardiovascular Diseases
- Association of monocyte to HDL cholesterol level with contrast induced nephropathy in STEMI patients treated with primary PCI
- Low concentrations of adropin are associated with endothelial dysfunction as assessed by flow-mediated dilatation in patients with metabolic syndrome
- Preoperative serum potassium predicts the clinical outcome after non-cardiac surgery
- Diabetes
- A simple colorimetric assay for measuring fructosamine 3 kinase activity
- Acknowledgment
- Acknowledgment
- Letters to the Editor
- Normalisation issues in glucose measurements using phlebotomy tubes with liquid additives
- Utility of dilution tests in investigating interference in the free thyroxine assay
- Plasminogen activator inhibitor-1 as a marker of cardiovascular response in professional mountain ultra-marathon runners
- Pitfalls of LSD screening assays: comparison of KIMS and CEDIA immunoassays with LC-MS
- A two site comparison of two point-of-care activated clotting time systems
- Detection of the common South-East Asian β0-thalassemia mutations in samples with borderline HbA2 levels
- Biclonal plasma cell myeloma with the simultaneous appearance of both secretory lambda and nonsecretory kappa monoclonal light chains
Articles in the same Issue
- Frontmatter
- Editorial
- Describing children’s changes using clinical chemistry analytes
- Review
- Assessment of vitamin D status – a changing landscape
- Opinion Papers
- Order of blood draw: Opinion Paper by the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE)
- The principles of Health Technology Assessment in laboratory medicine
- Genetics and Molecular Diagnostics
- Functional polymorphism of lncRNA MALAT1 contributes to pulmonary arterial hypertension susceptibility in Chinese people
- General Clinical Chemistry and Laboratory Medicine
- Influence of pneumatic tube system transport on routinely assessed and spectrophotometric cerebrospinal fluid parameters
- Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples
- Evaluation of the first fully automated immunoassay method for the measurement of stimulating TSH receptor autoantibodies in Graves’ disease
- Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children
- Activity and expression of dipeptidyl peptidase IV on peripheral blood mononuclear cells in patients with early steroid and disease modifying antirheumatic drugs naïve rheumatoid arthritis
- Hematology and Coagulation
- Neutrophil CD64 Index as a superior biomarker for early diagnosis of infection in febrile patients in the hematology department
- Dilute Russell’s viper venom time reagents in lupus anticoagulant testing: a well-considered choice
- Reference Values and Biological Variations
- Pediatric reference intervals for alkaline phosphatase
- Cancer Diagnostics
- Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target
- CDH1 (E-cadherin) expression independently affects clinical outcome in acute myeloid leukemia with normal cytogenetics
- Cardiovascular Diseases
- Association of monocyte to HDL cholesterol level with contrast induced nephropathy in STEMI patients treated with primary PCI
- Low concentrations of adropin are associated with endothelial dysfunction as assessed by flow-mediated dilatation in patients with metabolic syndrome
- Preoperative serum potassium predicts the clinical outcome after non-cardiac surgery
- Diabetes
- A simple colorimetric assay for measuring fructosamine 3 kinase activity
- Acknowledgment
- Acknowledgment
- Letters to the Editor
- Normalisation issues in glucose measurements using phlebotomy tubes with liquid additives
- Utility of dilution tests in investigating interference in the free thyroxine assay
- Plasminogen activator inhibitor-1 as a marker of cardiovascular response in professional mountain ultra-marathon runners
- Pitfalls of LSD screening assays: comparison of KIMS and CEDIA immunoassays with LC-MS
- A two site comparison of two point-of-care activated clotting time systems
- Detection of the common South-East Asian β0-thalassemia mutations in samples with borderline HbA2 levels
- Biclonal plasma cell myeloma with the simultaneous appearance of both secretory lambda and nonsecretory kappa monoclonal light chains
