N Latex FLC serum free light-chain assays in patients with renal impairment
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Joannes F.M. Jacobs
Abstract
Background: The aim of this study was to establish ranges for N Latex free light-chain (FLC) monoclonal-based nephelometric assays in patients with renal impairment.
Methods: In this retrospective study, serum samples from 284 patients with chronic kidney disease (CKD) stages 1–5 were measured with N Latex and Freelite™ FLC reagents on the Siemens BN™II system and compared with controls without renal impairment.
Results: Both κFLC and λFLC concentrations increased with the N Latex FLC and the Freelite™ assays with each increment in CKD stage. No difference was found in FLC κ concentrations between the two methods. In patients with renal failure, N Latex FLC detected higher concentrations of λFLC (CKD5 median, 128 mg/L; 95% range, 43–302) compared with Freelite™ (89.5 mg/L, 35–197) (p<0.0001). This resulted in significantly different κ/λ ratios in patients with CKD for the two tests. The Freelite™ κ/λ ratio in the CKD5 group (median, 1.22; min–max, 0.22–2.70) was significantly increased compared with healthy controls (p<0.0001), and several individual samples were outside the reference range for healthy controls (0.26–1.65). In contrast, none of the 284 patients with CKD had an FLC κ/λ ratio exceeding the N Latex reference limits for healthy controls (0.31–1.56). The N Latex FLC κ/λ ratio in the CKD5 group (0.69, 0.32–1.54) was significantly lower compared with the control group (p<0.0001).
Conclusions: These findings demonstrate that the N Latex FLC κ/λ ratio in patients with renal failure did not differ from the reference limits for healthy controls.
References
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©2014 by Walter de Gruyter Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorials
- Quo vadis, biomarkers?
- Translational researchers beware! Unreliable commercial immunoassays (ELISAs) can jeopardize your research
- Reviews
- Tracing a roadmap for vitamin B12 testing using the health technology assessment approach
- Meta-analysis: diagnostic accuracy of anti-cyclic citrullinated peptide 2 antibody and anti-cyclic citrullinated peptide 3 antibody in rheumatoid arthritis
- Perspectives
- Present and future of cancer biomarkers
- Opinion Paper
- A repository for “rare” tumor markers?
- Genetics and Molecular Diagnostics
- Characterization of a complex CYP2D6 genotype that caused an AmpliChip CYP450 Test® no-call in the clinical setting
- General Clinical Chemistry and Laboratory Medicine
- Tighter precision target required for lactate testing in patients with lactic acidosis
- The revised Lund-Malmö GFR estimating equation outperforms MDRD and CKD-EPI across GFR, age and BMI intervals in a large Swedish population
- Validation of a point-of-care (POC) lactate testing device for fetal scalp blood sampling during labor: clinical considerations, practicalities and realities
- Dabigatran, rivaroxaban, apixaban, argatroban and fondaparinux and their effects on coagulation POC and platelet function tests
- Evaluation of clinical cases in External Quality Assessment Scheme (EQAS) for the urinary sediment
- N Latex FLC serum free light-chain assays in patients with renal impairment
- A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction
- A sensitive chemiluminescence imaging immunoassay for simultaneous detection of serum oxidized lipoprotein(a) and low density lipoprotein
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- Cancer Diagnostics
- An epidemiology-based model to estimate the rate of inappropriateness of tumor marker requests
- Evaluation of INK4A promoter methylation using pyrosequencing and circulating cell-free DNA from patients with hepatocellular carcinoma
- Cardiovascular Diseases
- The in vitro stability of novel cardiovascular and sepsis biomarkers at ambient temperature
- Analytical evaluation of the automated galectin-3 assay on the Abbott ARCHITECT immunoassay instruments
- Infectious Diseases
- Serum miR-122 levels are related to coagulation disorders in sepsis patients
- Letter to the Editors
- Effect of storage conditions and freeze/thaw cycles on serum and plasma levels of anti-acetylcholine receptor (AChR) antibody
- Enzymatic isotope dilution mass spectrometry (IDMS) traceable serum creatinine is preferable over Jaffe in neonates and young infants
- Vitamin D, atopy, immunity
- 25-Hydroxy vitamin D levels in chronic urticaria and its correlation with disease severity from a tertiary care centre in South India
- High prevalence of anti-thyroid antibodies associated with a low vitamin D status in a pediatric cohort
- Serum melatonin levels in psoriasis and associated depressive symptoms
- Congress Abstracts
- ISMD2014 Tenth International Symposium on Molecular Diagnostics
