Serum miR-122 levels are related to coagulation disorders in sepsis patients

Hui-Juan Wang; Jie Deng; Jing-Yang Wang; Peng-Jun Zhang; Zhang Xin; Kun Xiao; Dan Feng; Yan-Hong Jia; You-Ning Liu; Li-Xin Xie

doi:10.1515/cclm-2013-0899

Article

Serum miR-122 levels are related to coagulation disorders in sepsis patients

Hui-Juan Wang , Jie Deng , Jing-Yang Wang , Peng-Jun Zhang , Zhang Xin , Kun Xiao , Dan Feng , Yan-Hong Jia , You-Ning Liu and Li-Xin Xie

Published/Copyright: January 14, 2014

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From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 52 Issue 6

Abstract

Background: Coagulation abnormalities may have a major impact on the outcome of sepsis in patients. This study aimed to explore the relationship between miRNA levels and coagulation disorders during sepsis.

Methods: Blood samples from 123 sepsis patients were collected on the day of admission and another 45 sepsis patients on days 1, 3, 5, 7, 10, and 14 following admission to the intensive care unit. miR-223, miR-15a, miR-16, miR-122, miR-193b*, and miR-483-5p levels were evaluated by quantitative reverse transcription polymerase chain reaction. Based on the International Society on Thrombosis and Haemostasis (ISTH) Disseminated Intravascular Coagulation (DIC) score, sepsis patients were divided into coagulation abnormal (CA) group and coagulation normal (CN) group.

Results: Only the levels of miR-122 were significantly higher in CA patients than in CN patients (p<0.001). Serum levels of miR-122 were correlated to the serum activated partial thromboplastin time (APTT) ratios (R=0.426, p=0.008) and the fibrinogen (FIB; R=0.398, p=0.008) and antithrombin III (R=0.913, p<0.001) levels. In addition, Pearson’s correlation coefficients for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) with miR-122 were 0.663 (p<0.001) and 0.445 (p=0.001), respectively. In the 45 patients, the miR-122 levels were significantly higher on day 1, 3, 7, and 10 in the CA group than in the CN group, and no difference in the ISTH-DIC scores was evident.

Conclusions: Serum levels of miR-122 were correlated to the coagulation disorder in sepsis patients.

Keywords: antithrombin III; coagulation abnormalities; hsa-miR-122; sepsis

Corresponding authors: You-Ning Liu and Li-Xin Xie, Department of Respiratory Medicine, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853 Beijing, P.R. China, Phone: +86 10 55499130, Fax: +86 10 55499130, E-mail: liuyn301@126.com; xielx@263.net

aHui-Juan Wang, Jie Deng and Jing-Yang Wang contributed equally to this study.

Acknowledgments

We wish to thank all of the doctors and nurses in the Department of Respiratory Diseases, the Emergency Department, and the Department of Surgery’s ICU for their support and assistance.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: This study was supported by the general program of the National Natural Science Foundation of China (81170008), and the general program of China’s 12th 5 Year Plan and its military (CWS11J094).

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2013-10-19

Accepted: 2013-11-21

Published Online: 2014-1-14

Published in Print: 2014-6-1

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Articles in the same Issue

https://doi.org/10.1515/cclm-2013-0899

Keywords for this article

antithrombin III; coagulation abnormalities; hsa-miR-122; sepsis