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Validity and reliability of the 13C-methionine breath test for the detection of moderate hyperhomocysteinemia in Mexican adults

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Veröffentlicht/Copyright: 7. März 2014
Clinical Chemistry and Laboratory Medicine
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine Band 52 Heft 5

Abstract

Background: Hyperhomocysteinemia (Hhcy) is considered an independent risk factor for vascular diseases and, more recently, for dementia. The methionine loading test (MLT) is useful for diagnosing additional subjects with moderate Hhcy. However, it is a complex and time-consuming procedure. A noninvasive test for the diagnosis of moderate Hhcy is desirable.

Methods: The study protocol consisted of three consecutive visits. During the first visit, we performed an MLT to characterize the Hhcy status of 75 healthy adult subjects. For the breath test protocol, we selected a subsample and assigned to the control group 17 subjects with fasting and post-loading homocysteine (Hcy) ≤12 and <42.3 μmol/L, respectively, and to the Hhcy group 16 subjects with fasting Hcy ≤12 and >42.3 μmol/L after loading. Selected subjects were requested to have a second visit to perform a breath test within 1–4 weeks following the MLT test and received an oral dose of 2.5 mg/kg of 1-13C-methionine dissolved in water. Breath samples were collected at basal, 20, 40, 60, 80, 100 and 120 min (test 1). The same procedure was repeated within 1 week (test 2).

Results: MLT was useful for diagnosing almost twice the number of individuals with Hhcy (24%) in comparison with the fasting determination alone (13.3%). The 13C-methionine breath test reported a sensitivity of 81.3% and a specificity of 64.7% against the MLT. The coefficient of variation between breath test 1 and breath test 2 was 9.0±5.4%.

Conclusions: The 13C-methionine breath test is a valid and reliable method for identifying subjects with moderate Hhcy.

Keywords: 13C-methionine breath test; hyperhomocysteinemia; methionine loading test
Corresponding author: Jorge Maldonado-Hernández, Medical Unit of Nutrition Research, 21st Century National Medical Center, Mexican Institute of Social Security, Av. Cuauhtémoc No. 330, Col. Doctores, 06720 Mexico City, Mexico, Phone/Fax: +52 55 56 27 69 44, E-mail:

Acknowledgments

We are grateful to the volunteers for their participation in this study. This study was supported by grants from Consejo Nacional de Ciencia y Tecnología (CONACYT: 86648-SALUD 2008-1) and from Fondo de Investigación en Salud (FIS/IMSS/PROT/623) and (FIS/IMSS/PROT/119).

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research support played no role in thestudy design; in the collection, analysis, and interpretationof data; in the writing of the report; or in the decision tosubmit the report for publication.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2013-10-1
Accepted: 2013-10-24
Published Online: 2014-3-7
Published in Print: 2014-5-1

©2014 by Walter de Gruyter Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Editorial
  3. Potential application of fetal epigenetic markers on the non-invasive prenatal detection of chromosomal abnormality
  4. Review
  5. Epigenetic mechanisms in bone
  6. Opinion Papers
  7. Circulating fetal cell-free DNA and prenatal molecular diagnostics: are we ready for consensus?
  8. Hepcidin levels in chronic hemodialysis patients: a critical evaluation
  9. D-dimer testing for suspected venous thromboembolism in the emergency department. Consensus document of AcEMC, CISMEL, SIBioC, and SIMeL
  10. General Clinical Chemistry and Laboratory Medicine
  11. Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma
  12. Non-invasive detection of fetal trisomy 21 using fetal epigenetic biomarkers with a high CpG density
  13. Development and validation of dried matrix spot sampling for the quantitative determination of amyloid β peptides in cerebrospinal fluid
  14. Instability of cerebrospinal fluid after delayed storage and repeated freezing: a holistic study by drop coating deposition Raman spectroscopy
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  16. Performance of urinary NGAL and L-FABP in predicting acute kidney injury and subsequent renal recovery: a cohort study based on major surgeries
  17. Rapid extraction, identification and quantification of drugs of abuse in hair by immunoassay and ultra-performance liquid chromatography tandem mass spectrometry
  18. Validity and reliability of the 13C-methionine breath test for the detection of moderate hyperhomocysteinemia in Mexican adults
  19. Quantitative detection of target cells using unghosted cells (UGCs) of DxH 800 (Beckman Coulter)
  20. Evaluation of different sized blood sampling tubes for thromboelastometry, platelet function, and platelet count
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  22. Routine use of the Ion Torrent AmpliSeq™ Cancer Hotspot Panel for identification of clinically actionable somatic mutations
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  26. Cardiovascular Diseases
  27. Red blood cell distribution width predicts survival in patients with Eisenmenger syndrome
  28. Low serum adropin is associated with coronary atherosclerosis in type 2 diabetic and non-diabetic patients
  29. Corrigendum
  30. Standardization and analytical goals for glycated hemoglobin measurement
  31. Letter to the Editors
  32. Do we need to worry about contamination by circulating fetal DNA?
  33. Stat testing utilization in clinical laboratories. National survey of Italian Society of Clinical Biochemistry and Molecular Biology (SIBioC)
  34. Aldosterone measurement with LiaisonXL automated system: remarks about reference range
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https://doi.org/10.1515/cclm-2013-0834
Schlagwörter für diesen Artikel
13C-methionine breath test; hyperhomocysteinemia; methionine loading test

Artikel in diesem Heft

  1. Frontmatter
  2. Editorial
  3. Potential application of fetal epigenetic markers on the non-invasive prenatal detection of chromosomal abnormality
  4. Review
  5. Epigenetic mechanisms in bone
  6. Opinion Papers
  7. Circulating fetal cell-free DNA and prenatal molecular diagnostics: are we ready for consensus?
  8. Hepcidin levels in chronic hemodialysis patients: a critical evaluation
  9. D-dimer testing for suspected venous thromboembolism in the emergency department. Consensus document of AcEMC, CISMEL, SIBioC, and SIMeL
  10. General Clinical Chemistry and Laboratory Medicine
  11. Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma
  12. Non-invasive detection of fetal trisomy 21 using fetal epigenetic biomarkers with a high CpG density
  13. Development and validation of dried matrix spot sampling for the quantitative determination of amyloid β peptides in cerebrospinal fluid
  14. Instability of cerebrospinal fluid after delayed storage and repeated freezing: a holistic study by drop coating deposition Raman spectroscopy
  15. Analysis of patients with γ-heavy chain disease by the heavy/light chain and free light chain assays
  16. Performance of urinary NGAL and L-FABP in predicting acute kidney injury and subsequent renal recovery: a cohort study based on major surgeries
  17. Rapid extraction, identification and quantification of drugs of abuse in hair by immunoassay and ultra-performance liquid chromatography tandem mass spectrometry
  18. Validity and reliability of the 13C-methionine breath test for the detection of moderate hyperhomocysteinemia in Mexican adults
  19. Quantitative detection of target cells using unghosted cells (UGCs) of DxH 800 (Beckman Coulter)
  20. Evaluation of different sized blood sampling tubes for thromboelastometry, platelet function, and platelet count
  21. Cancer Diagnostics
  22. Routine use of the Ion Torrent AmpliSeq™ Cancer Hotspot Panel for identification of clinically actionable somatic mutations
  23. Analytical performance of a new one-step quantitative prostate-specific antigen assay, the FREND™ PSA Plus
  24. Relationship between prostate-specific antigen kinetics and detection rate of radiolabelled choline PET/CT in restaging prostate cancer patients: a meta-analysis
  25. Methylation of OPCML promoter in ovarian cancer tissues predicts poor patient survival
  26. Cardiovascular Diseases
  27. Red blood cell distribution width predicts survival in patients with Eisenmenger syndrome
  28. Low serum adropin is associated with coronary atherosclerosis in type 2 diabetic and non-diabetic patients
  29. Corrigendum
  30. Standardization and analytical goals for glycated hemoglobin measurement
  31. Letter to the Editors
  32. Do we need to worry about contamination by circulating fetal DNA?
  33. Stat testing utilization in clinical laboratories. National survey of Italian Society of Clinical Biochemistry and Molecular Biology (SIBioC)
  34. Aldosterone measurement with LiaisonXL automated system: remarks about reference range
  35. Better blood collection tubes for plasma glucose: ready for prime time?
  36. Upregulation of cytokines and IL-17 in patients with hereditary angioedema
  37. Underestimation of platelet count on magnesium salt-anticoagulated samples
  38. Extensive elevation of BNP but not NT-ProBNP in a patient with advanced urothelial carcinoma in absence of cardiac failure and volume overload
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