Analysis of patients with γ-heavy chain disease by the heavy/light chain and free light chain assays
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Erin Kaleta
Abstract
Background: The objective of this study was to evaluate the performance of the heavy/light chain and free light chain immunoassays in patients with heavy chain disease, and to assess the ability of the heavy/light chain assay to measure and confirm the abnormal, truncated heavy chain.
Methods: Frozen serum samples from 15 γ-heavy chain disease patients were tested for IgGκ, IgGλ, total IgG, free light chains, and M-spike concentrations.
Results: The (Gκ+Gλ)/IgGtotal ratio for these 15 patients ranged from 0.02 to 0.80. The 10 patients with IgG concentrations above 1 g/dL all had ratios below 0.3 indicating that a substantial portion of IgG was not quantitated by the Gκ and Gλ reagents. The average M-spike was 1.61 g/dL and the average calculated abnormal γ-chain concentration was 2.94 g/dL. Additionally, free light chain analysis revealed the presence of monoclonal free κ light chain in three of the 15 patients.
Conclusions: This study demonstrates utility of a nephelometric assay to identify truncated immunoglobulin heavy chains in γ-HCD and that 20% of these patients also have monoclonal free light chain.
References
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©2014 by Walter de Gruyter Berlin/Boston
Articles in the same Issue
- Frontmatter
- Editorial
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- Review
- Epigenetic mechanisms in bone
- Opinion Papers
- Circulating fetal cell-free DNA and prenatal molecular diagnostics: are we ready for consensus?
- Hepcidin levels in chronic hemodialysis patients: a critical evaluation
- D-dimer testing for suspected venous thromboembolism in the emergency department. Consensus document of AcEMC, CISMEL, SIBioC, and SIMeL
- General Clinical Chemistry and Laboratory Medicine
- Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma
- Non-invasive detection of fetal trisomy 21 using fetal epigenetic biomarkers with a high CpG density
- Development and validation of dried matrix spot sampling for the quantitative determination of amyloid β peptides in cerebrospinal fluid
- Instability of cerebrospinal fluid after delayed storage and repeated freezing: a holistic study by drop coating deposition Raman spectroscopy
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- Rapid extraction, identification and quantification of drugs of abuse in hair by immunoassay and ultra-performance liquid chromatography tandem mass spectrometry
- Validity and reliability of the 13C-methionine breath test for the detection of moderate hyperhomocysteinemia in Mexican adults
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- Cancer Diagnostics
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- Letter to the Editors
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Articles in the same Issue
- Frontmatter
- Editorial
- Potential application of fetal epigenetic markers on the non-invasive prenatal detection of chromosomal abnormality
- Review
- Epigenetic mechanisms in bone
- Opinion Papers
- Circulating fetal cell-free DNA and prenatal molecular diagnostics: are we ready for consensus?
- Hepcidin levels in chronic hemodialysis patients: a critical evaluation
- D-dimer testing for suspected venous thromboembolism in the emergency department. Consensus document of AcEMC, CISMEL, SIBioC, and SIMeL
- General Clinical Chemistry and Laboratory Medicine
- Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma
- Non-invasive detection of fetal trisomy 21 using fetal epigenetic biomarkers with a high CpG density
- Development and validation of dried matrix spot sampling for the quantitative determination of amyloid β peptides in cerebrospinal fluid
- Instability of cerebrospinal fluid after delayed storage and repeated freezing: a holistic study by drop coating deposition Raman spectroscopy
- Analysis of patients with γ-heavy chain disease by the heavy/light chain and free light chain assays
- Performance of urinary NGAL and L-FABP in predicting acute kidney injury and subsequent renal recovery: a cohort study based on major surgeries
- Rapid extraction, identification and quantification of drugs of abuse in hair by immunoassay and ultra-performance liquid chromatography tandem mass spectrometry
- Validity and reliability of the 13C-methionine breath test for the detection of moderate hyperhomocysteinemia in Mexican adults
- Quantitative detection of target cells using unghosted cells (UGCs) of DxH 800 (Beckman Coulter)
- Evaluation of different sized blood sampling tubes for thromboelastometry, platelet function, and platelet count
- Cancer Diagnostics
- Routine use of the Ion Torrent AmpliSeq™ Cancer Hotspot Panel for identification of clinically actionable somatic mutations
- Analytical performance of a new one-step quantitative prostate-specific antigen assay, the FREND™ PSA Plus
- Relationship between prostate-specific antigen kinetics and detection rate of radiolabelled choline PET/CT in restaging prostate cancer patients: a meta-analysis
- Methylation of OPCML promoter in ovarian cancer tissues predicts poor patient survival
- Cardiovascular Diseases
- Red blood cell distribution width predicts survival in patients with Eisenmenger syndrome
- Low serum adropin is associated with coronary atherosclerosis in type 2 diabetic and non-diabetic patients
- Corrigendum
- Standardization and analytical goals for glycated hemoglobin measurement
- Letter to the Editors
- Do we need to worry about contamination by circulating fetal DNA?
- Stat testing utilization in clinical laboratories. National survey of Italian Society of Clinical Biochemistry and Molecular Biology (SIBioC)
- Aldosterone measurement with LiaisonXL automated system: remarks about reference range
- Better blood collection tubes for plasma glucose: ready for prime time?
- Upregulation of cytokines and IL-17 in patients with hereditary angioedema
- Underestimation of platelet count on magnesium salt-anticoagulated samples
- Extensive elevation of BNP but not NT-ProBNP in a patient with advanced urothelial carcinoma in absence of cardiac failure and volume overload
