Startseite Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test
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Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test

  • Milan Dobric EMAIL logo , Vojislav Giga , Branko Beleslin , Svetlana Ignjatovic , Ivana Paunovic , Jelena Stepanovic , Ana Djordjevic-Dikic , Jelena Kostic , Ivana Nedeljkovic , Milan Nedeljkovic , Milorad Tesic , Marijana Dajak und Miodrag Ostojic
Veröffentlicht/Copyright: 28. Mai 2013
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Clinical Chemistry and Laboratory Medicine (CCLM)
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine (CCLM) Band 51 Heft 10

Abstract

Background: Glycogen phosphorylase BB (GPBB) is released from cardiac cells during myocyte damage. Previous studies have shown contradictory results regarding the relation of enzyme release and reversible myocardial ischemia. The aim of this study was to determine the plasma kinetics of GPBB as a response to the exercise stress echocardiographic test (ESET), and to define the relationship between myocardial ischemia and enzyme plasma concentrations.

Methods: We studied 46 consecutive patients undergoing ESET, with recent coronary angiography. In all patients, a submaximal stress echo test according to Bruce protocol was performed. Concentration of GPBB was measured in peripheral blood that was sampled 5 min before and 10, 30 and 60 min after ESET.

Results: There was significant increase of GPBB concentration after the test (p=0.021). Significant increase was detected 30 min (34.9% increase, p=0.021) and 60 min (34.5% increase, p=0.016) after ESET. There was no significant effect of myocardial ischemia on GPBB concentrations (p=0.126), and no significant interaction between sampling intervals and myocardial ischemia, suggesting a similar release profile of GPBB in ischemic and non-ischemic conditions (p=0.558). Patients in whom ESET was terminated later (stages 4 or 5 of standard Bruce protocol; n=13) had higher GPBB concentrations than patients who terminated ESET earlier (stages 1, 2 or 3; n=33) (p=0.049). Baseline GPBB concentration was not correlated to any of the patients’ demographic, clinical and hemodynamic characteristics.

Conclusions: GPBB plasma concentration increases after ESET, and it is not related to inducible myocardial ischemia. However, it seems that GPBB release during ESET might be related to exercise load/duration.

Keywords: exercise stress echo test; glycogen phosphorylase isoenzyme BB; myocardial ischemia; pharmacokinetics
Corresponding author: Milan Dobric, MD, Department for Functional Diagnostics and Hemodynamics, Division of Cardiology, Clinical Center of Serbia, Koste Todorovica 8, 11000 Belgrade, Serbia, Phone/Fax: +381 11 3663291, E-mail:

This study was supported partially by the grants III41022 and ON175036 of the Ministry of Science of the Republic of Serbia. Authors are also thankful to Vesna Mandic for logistic support of this study.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2013-02-08
Accepted: 2013-05-05
Published Online: 2013-05-28
Published in Print: 2013-10-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2013-0109
Schlagwörter für diesen Artikel
exercise stress echo test; glycogen phosphorylase isoenzyme BB; myocardial ischemia; pharmacokinetics

Artikel in diesem Heft

  1. Letters to the Editor
  2. Effect of hemoglobin Porto Alegre on glycated hemoglobin (HbA1c) measurement with the HA-8160 high performance liquid chromatography method
  3. Influence of sample type and delayed separation from cells on the measurement of B-type natriuretic peptide with the Architect system
  4. Efficient use of basic biochemical methods to prove the presence of monoclonal protein in the clinical diagnosis of malignant monoclonal gammopathy
  5. Ammonia does not interfere with the Diazyme adenosine deaminase test
  6. Interindividual variability of hemolysis in plasma samples during pneumatic tube system transport
  7. Kidney stones in a southeast European population from Bulgaria
  8. The influence of non-specificity of the creatinine assay on eGFR
  9. Reference values for alanine aminotransferase, α-amylase, aspartate aminotransferase, γ-glutamyltransferase and lactate dehydrogenase measured according to the IFCC standardization during uncomplicated pregnancy
  10. Congress Abstracts
  11. Congress of Clinical Chemistry and Laboratory Medicine / 10th Annual Meeting of the German Society for Clinical Chemistry and Laboratory Medicine (DGKL), Dresden, Germany, 23rd–26th October, 2013*)
  12. Masthead
  13. Masthead
  14. Editorials
  15. Stabilization of glucose in blood samples: out with the old, in with the new
  16. Component-resolved diagnostics: laboratory results are not enough
  17. Reviews
  18. Molecular basis and clonal evolution of myeloproliferative neoplasms
  19. Current and future use of “dried blood spot” analyses in clinical chemistry
  20. Opinion Papers
  21. Staging chronic kidney disease and estimating glomerular filtration rate: an opinion paper about the new international recommendations
  22. Age, stage and biomarkers for the definition of CKD: a construction in progress
  23. Guidelines and Recommendations
  24. Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review
  25. General Clinical Chemistry and Laboratory Medicine
  26. Citric/citrate buffer: an effective antiglycolytic agent
  27. Relationship between coffee consumption, oxidant status, and antioxidant potential in the Japanese general population
  28. Investigation of apparent non-albuminuric proteinuria in a primary care population
  29. Method-specific differences in plasma fibroblast growth factor 23 measurement using four commercial ELISAs
  30. Technical and clinical evaluation of the VITROS® Immunodiagnostic Products 25-OH Vitamin D Total Assay – comparison with marketed automated immunoassays and a liquid chromatography-tandem mass spectrometry method
  31. Isolated IgE reactivity to native walnut vicilin-like protein (nJug r 2) on ISAC™ microarray is due to cross-reactive carbohydrate epitopes
  32. Reference Values and Biological Variations
  33. A systematic review of data on biological variation for alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transferase
  34. IDS iSYS automated intact procollagen-1-N-terminus pro-peptide assay: method evaluation and reference intervals in adults and children
  35. Cardiovascular Diseases
  36. High-sensitivity cardiac troponin T and N-terminal pro-B-type natriuretic peptide predict mortality in stable coronary artery disease: results from the Ludwigshafen Risk and Cardiovascular Health (LURIC) study
  37. Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test
  38. A novel method for the direct determination of heparin concentration during cardiopulmonary bypass surgery
  39. Diabetes
  40. External quality assessment of HbA1c and its effect on comparison between Swedish pediatric diabetes clinics. Experiences from the Swedish pediatric diabetes quality register (Swediabkids) and Equalis
  41. Infectious Diseases
  42. Presepsin (sCD14-ST) in preoperative diagnosis of abdominal sepsis
  43. Errata
  44. Recommendations for clinical laboratory science reports regarding properties, units, and symbols: the NPU format
  45. Standardization and analytical goals for glycated hemoglobin measurement
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