Startseite Medizin Plasma choline and betaine correlate with serum folate, plasma S-adenosyl-methionine and S-adenosyl-homocysteine in healthy volunteers
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Plasma choline and betaine correlate with serum folate, plasma S-adenosyl-methionine and S-adenosyl-homocysteine in healthy volunteers

  • Apolline Imbard EMAIL logo , Yvo M. Smulders , Rob Barto , Desiree E.C. Smith , Robert M. Kok , Cornelis Jakobs und Henk J. Blom
Veröffentlicht/Copyright: 12. September 2012
Clinical Chemistry and Laboratory Medicine
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine Band 51 Heft 3

Abstract

Background: Choline is essential for mammalian cell function. It plays a critical role in cell membrane integrity, neurotransmission, cell signaling and lipid metabolism. Moreover, choline is involved in methylation in two ways: a) its synthesis requires methyl groups donated by S-adenosyl-methionine (AdoMet); and b) choline oxidation product betaine methylates homocysteine (Hcy) to methionine (Met) and produces dimethylglycine. This later donates one carbon units to tetrahydrofolate (THF).

Methods: To evaluate the correlations of choline and betaine with folate, AdoMet, S-anenosyl-homocysteine (AdoHcy), total homocysteine (tHcy), and DNA methylation, choline, betaine and dimethylglycine were measured by LC-MS/MS in plasma of 109 healthy volunteers, in whom folate, AdoMet, AdoHcy, tHcy, and DNA methylation have previously been reported.

Results: Using a bivariate model, choline and betaine showed strong positive correlations with folate (r=0.346 and r=0.226), AdoHcy (r=0.468 and r=0.296), and correlated negatively with AdoMet/AdoHcy ratio (r=–0.246 and r=–0.379). Only choline was positively correlated with AdoMet (r=0.453). Using a multivariate linear regression model, choline correlated strongly with folate (β=17.416), AdoMet (β=61.272), and AdoHcy (β=9.215). Betaine correlated positively with folate (β=0.133) and negatively with tHcy (β=–0.194) ratio. Choline is an integral part of folate and methylation pathways.

Conclusions: Our data highlight the importance of integrating choline in studies concerning addressing pathological conditions related to folate, homocysteine and methylation metabolism.

Keywords: betaine; choline; folate; homocysteine; S-anenosyl-homocysteine; S-anenosyl-methionine
Corresponding author: Apolline Imbard, Biochemistry-Hormonology Laboratory, Robert Debré Hospital, 48 Boulevard Serurier, 75019 Paris, France Phone: +33 01 40034722, Fax: +33 01 40034790

The authors would like to thank the volunteers from the hospital personnel for their participation in this study.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2012-5-13
Accepted: 2012-8-18
Published Online: 2012-09-12
Published in Print: 2013-03-01

©2013 by Walter de Gruyter Berlin Boston

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Editorial
  4. Special issue on advances and controversies in B vitamins and choline
  5. Reviews
  6. B-Vitamin dependent methionine metabolism and alcoholic liver disease
  7. Metabolic crosstalk between choline/1-carbon metabolism and energy homeostasis
  8. Proteomics of vitamin B12 processing
  9. Unexpected high plasma cobalamin/Proposal for a diagnostic strategy
  10. Clinical recognition and aspects of the cerebral folate deficiency syndromes
  11. Choline-containing phospholipids: relevance to brain functional pathways
  12. The ‘golden age’ of DNA methylation in neurodegenerative diseases
  13. Mechanisms of the beneficial effects of vitamin B6 and pyridoxal 5-phosphate on cardiac performance in ischemic heart disease
  14. The diagnostic utility of folate receptor autoantibodies in blood
  15. Red cell or serum folate: what to do in clinical practice?
  16. Formate: an essential metabolite, a biomarker, or more?
  17. The role of homocysteine in bone remodeling
  18. Mini Reviews
  19. Neuroprotective actions of perinatal choline nutrition
  20. Normal prions as a new target of cobalamin (vitamin B12) in rat central nervous system
  21. Molecular mechanisms underlying the potentially adverse effects of folate
  22. Betaine homocysteine methyltransferase (BHMT)-dependent remethylation pathway in human healthy and tumoral liver
  23. Hydrogen sulfide as an oxygen sensor
  24. Opinion Paper
  25. B vitamin therapy for homocysteine: renal function and vitamin B12 determine cardiovascular outcomes
  26. Research Articles
  27. One year B and D vitamins supplementation improves metabolic bone markers
  28. Effect of 1 year B and D vitamin supplementation on LINE-1 repetitive element methylation in older subjects
  29. Aqueous humor glycation marker and plasma homocysteine in macular degeneration
  30. Homocysteine plasma levels in patients treated with antiepileptic drugs depend on folate and vitamin B12 serum levels, but not on genetic variants of homocysteine metabolism
  31. Trends in clinical laboratory homocysteine testing from 1997 to 2010: the impact of evidence on clinical practice at a single institution
  32. Three family members with elevated plasma cobalamin, transcobalamin and soluble transcobalamin receptor (sCD320)
  33. Plasma choline and betaine correlate with serum folate, plasma S-adenosyl-methionine and S-adenosyl-homocysteine in healthy volunteers
  34. Plasma homocysteine and vitamin B12 serum levels, red blood cell folate concentrations, C677T methylenetetrahydrofolate reductase gene mutation and risk of recurrent miscarriage: a case-control study in Spain
https://doi.org/10.1515/cclm-2012-0302
Schlagwörter für diesen Artikel
betaine; choline; folate; homocysteine; S-anenosyl-homocysteine; S-anenosyl-methionine

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Editorial
  4. Special issue on advances and controversies in B vitamins and choline
  5. Reviews
  6. B-Vitamin dependent methionine metabolism and alcoholic liver disease
  7. Metabolic crosstalk between choline/1-carbon metabolism and energy homeostasis
  8. Proteomics of vitamin B12 processing
  9. Unexpected high plasma cobalamin/Proposal for a diagnostic strategy
  10. Clinical recognition and aspects of the cerebral folate deficiency syndromes
  11. Choline-containing phospholipids: relevance to brain functional pathways
  12. The ‘golden age’ of DNA methylation in neurodegenerative diseases
  13. Mechanisms of the beneficial effects of vitamin B6 and pyridoxal 5-phosphate on cardiac performance in ischemic heart disease
  14. The diagnostic utility of folate receptor autoantibodies in blood
  15. Red cell or serum folate: what to do in clinical practice?
  16. Formate: an essential metabolite, a biomarker, or more?
  17. The role of homocysteine in bone remodeling
  18. Mini Reviews
  19. Neuroprotective actions of perinatal choline nutrition
  20. Normal prions as a new target of cobalamin (vitamin B12) in rat central nervous system
  21. Molecular mechanisms underlying the potentially adverse effects of folate
  22. Betaine homocysteine methyltransferase (BHMT)-dependent remethylation pathway in human healthy and tumoral liver
  23. Hydrogen sulfide as an oxygen sensor
  24. Opinion Paper
  25. B vitamin therapy for homocysteine: renal function and vitamin B12 determine cardiovascular outcomes
  26. Research Articles
  27. One year B and D vitamins supplementation improves metabolic bone markers
  28. Effect of 1 year B and D vitamin supplementation on LINE-1 repetitive element methylation in older subjects
  29. Aqueous humor glycation marker and plasma homocysteine in macular degeneration
  30. Homocysteine plasma levels in patients treated with antiepileptic drugs depend on folate and vitamin B12 serum levels, but not on genetic variants of homocysteine metabolism
  31. Trends in clinical laboratory homocysteine testing from 1997 to 2010: the impact of evidence on clinical practice at a single institution
  32. Three family members with elevated plasma cobalamin, transcobalamin and soluble transcobalamin receptor (sCD320)
  33. Plasma choline and betaine correlate with serum folate, plasma S-adenosyl-methionine and S-adenosyl-homocysteine in healthy volunteers
  34. Plasma homocysteine and vitamin B12 serum levels, red blood cell folate concentrations, C677T methylenetetrahydrofolate reductase gene mutation and risk of recurrent miscarriage: a case-control study in Spain
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