Comparison of selenium-induced antioxidant responses and bioaccumulation in two strains of the halotolerant alga Dunaliella salina
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Aida Hamidkhani
Aida Hamidkhani is a PhD student of microbial biotechnology at Alzahra University. She has a BSc in marine biology from Shahid Beheshti University (2010). She obtained her Master’s degree in the field of microbial biotechnology from Alzahra University in 2012. She has seven years of work experience in algal biotechnology and is a specialist in micro algal isolation and cultivation.
Dr.
Ezat Asgarani is an associate professor of Department of Biotechnology, Faculty of Biological Sciences, Alzahra University. She has 19 years experience in teaching and research in the fields of molecular genetics and biodiversity. At present she is focused on gray biotechnology.
,
Azra Saboora
Dr.
Azra Saboora is an associate professor of Department of Botany, Faculty of Biological Sciences, Alzahra University. Her research interests include plant stress, secondary metabolites and pharmaceutical compounds, phytoremediation, and molecular biology in plants
Dr.
Mohammad Amin Hejazi is an associate professor and head of Food Biotechnology Research Institute, Agricultural Biotechnology Research Institute of Iran. His research interests include food biotechnology and algal biotechnology. He is active in the field of engineering of bioprocesses for production of valuable compounds by microalgae including screening of native algal strains for biotechnological applications, design and development of photobioreactors, and bioprocesses optimization. He has been awarded several titles on international and national levels for his scientific efforts.
Abstract
Selenium water pollution is an increasing environmental problem that requires investigation of cellular responses of aquatic primary producer organisms, especially algae. Industrial wastewater with selenium contamination is often coupled with high salinity (60–70). In this study, the biochemical responses of two strains of the halotolerant alga (Dunaliella salina Hoze-soltan and Dunaliella salina CCAP 19/18) to different selenium concentrations were evaluated. Although at high selenium concentrations both strains showed lipid peroxidation and cell number reduction, Dunaliella salina Hoze-soltan was less affected. Higher selenium tolerance in this strain might be attributed to the better activity of resistance responses like proline, total reducing sugar, superoxide dismutase (SOD) and peroxidase (POX), even at the high selenium concentrations. Catalase (CAT) had no significant role for protection against selenium toxicity as its activity declined in both strains with rising selenium concentration. Both strains accumulated selenium intracellularly, but the accumulation was about three-fold higher in Dunaliella salina Hoze-soltan than in the other strain. It can be concluded that Dunaliella salina Hoze-soltan may be a better candidate for selenium bioremediation of a high salinity environment. The data obtained from this study could be useful for improvement of algal ability for high efficiency selenium bioremediation in hypersaline environments.
Funding source: Alzahra University
Funding source: Biotechnology Development Council of the Islamic Republic of Iran
Award Identifier / Grant number: 970203
About the authors
Aida Hamidkhani is a PhD student of microbial biotechnology at Alzahra University. She has a BSc in marine biology from Shahid Beheshti University (2010). She obtained her Master’s degree in the field of microbial biotechnology from Alzahra University in 2012. She has seven years of work experience in algal biotechnology and is a specialist in micro algal isolation and cultivation.
Dr.
Ezat Asgarani is an associate professor of Department of Biotechnology, Faculty of Biological Sciences, Alzahra University. She has 19 years experience in teaching and research in the fields of molecular genetics and biodiversity. At present she is focused on gray biotechnology.
Dr.
Azra Saboora is an associate professor of Department of Botany, Faculty of Biological Sciences, Alzahra University. Her research interests include plant stress, secondary metabolites and pharmaceutical compounds, phytoremediation, and molecular biology in plants
Dr.
Mohammad Amin Hejazi is an associate professor and head of Food Biotechnology Research Institute, Agricultural Biotechnology Research Institute of Iran. His research interests include food biotechnology and algal biotechnology. He is active in the field of engineering of bioprocesses for production of valuable compounds by microalgae including screening of native algal strains for biotechnological applications, design and development of photobioreactors, and bioprocesses optimization. He has been awarded several titles on international and national levels for his scientific efforts.
Acknowledgments
The authors appreciate Tahghighat Arian Gostar Co. for kindly offering equipment and materials during this project.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This study was financially supported by the Alzahra University, Tehran, Iran, and by grant no. 970203 of the Biotechnology Development Council of the Islamic Republic of Iran.
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and ecology
- Different light and temperature requirements for survival and growth in three species of Kallymeniaceae (Rhodophyta) from the Mediterranean Sea
- The effect of salinity on aspects of the early development of the kelp species Undaria pinnatifida and Saccorhiza polyschides
- Increased ammonium levels occurring during benthic algal blooms are potentially toxic to fucalean algae
- Comparison of selenium-induced antioxidant responses and bioaccumulation in two strains of the halotolerant alga Dunaliella salina
- Taxonomy/phylogeny and biogeography
- Culturable fungi associated with the marine shallow-water hydrothermal vent crab Xenograpsus testudinatus at Kueishan Island, Taiwan
- Chemistry and applications
- Sulfoquinovose metabolism in marine algae
- Biochemical characterization of eight Greek algae as candidate species for local seaweed cultivation
- Trace element concentrations in seaweeds of the Arabian Gulf identified by morphology and DNA barcodes
Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and ecology
- Different light and temperature requirements for survival and growth in three species of Kallymeniaceae (Rhodophyta) from the Mediterranean Sea
- The effect of salinity on aspects of the early development of the kelp species Undaria pinnatifida and Saccorhiza polyschides
- Increased ammonium levels occurring during benthic algal blooms are potentially toxic to fucalean algae
- Comparison of selenium-induced antioxidant responses and bioaccumulation in two strains of the halotolerant alga Dunaliella salina
- Taxonomy/phylogeny and biogeography
- Culturable fungi associated with the marine shallow-water hydrothermal vent crab Xenograpsus testudinatus at Kueishan Island, Taiwan
- Chemistry and applications
- Sulfoquinovose metabolism in marine algae
- Biochemical characterization of eight Greek algae as candidate species for local seaweed cultivation
- Trace element concentrations in seaweeds of the Arabian Gulf identified by morphology and DNA barcodes
