Genetic diversity and biogeography in Chaetomorpha melagonium (Ulvophyceae, Cladophorales) based on internal transcribed spacer (ITS rDNA) sequences
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Christian Boedeker
Christian Boedeker is currently a postdoc at Victoria University of Wellington and has been studying the green algal order Cladophorales for more than 10 years.
,
Frederik Leliaert
Frederik Leliaert is a research director at the Botanic Garden Meise and visiting professor at Ghent University. He uses molecular, morphological, ecological and geographic data to resolve phylogenetic relationships, test species boundaries and explore the biogeographical history in marine macroalgae.
Giuseppe Zuccarello is interested in the taxonomy, evolution and speciation of algae. He received a PhD from the University of California, Berkeley. He has been the president of the International Phycological Society and is currently an associate professor at Victoria University of Wellington and has published over 120 peer-reviewed papers.
Abstract
Chaetomorpha melagonium is a morphologically distinct species of green algae that occurs throughout the North Atlantic, the North Pacific and the Arctic Ocean. In this study, we analyzed the intraspecific genetic diversity among 14 samples of C. melagonium from across the distribution range based on nuclear large subunit ribosomal DNA (LSU rDNA) and rDNA internal transcribed spacer (ITS) DNA sequences. All samples had identical LSU sequences. The ITS sequences had very few mutations that nevertheless divided the specimens into two groups: one included samples from Iceland, Svalbard, Massachusetts and Alaska with identical ITS sequences; members of this group differed in samples from Europe (France, Germany, Scotland, Sweden, and Wales) by three mutations (two point mutations and one five base pair indel). The European specimens had identical ITS sequences with the exception of a single sample from Brittany that differed by one base pair. The maximum ITS sequence divergence within the samples of C. melagonium was less than 0.5%. This low intraspecific variation in the frequently used highly variable ITS region is discussed in the context of past geological and climatic scenarios.
About the authors
Christian Boedeker is currently a postdoc at Victoria University of Wellington and has been studying the green algal order Cladophorales for more than 10 years.
Frederik Leliaert is a research director at the Botanic Garden Meise and visiting professor at Ghent University. He uses molecular, morphological, ecological and geographic data to resolve phylogenetic relationships, test species boundaries and explore the biogeographical history in marine macroalgae.
Giuseppe Zuccarello is interested in the taxonomy, evolution and speciation of algae. He received a PhD from the University of California, Berkeley. He has been the president of the International Phycological Society and is currently an associate professor at Victoria University of Wellington and has published over 120 peer-reviewed papers.
Acknowledgments
We are grateful to Kai Bischof, Gayle Hansen, Christine Maggs, Jan Rueness, Loes Venekamp and Andreas Wagner for providing samples. CB acknowledges the Royal Society of New Zealand (Marsden Fund) for a postdoctoral scholarship.
References
Baer, C.F., M.M. Miyamoto and D.R. Denver. 2007. Mutation rate variation in multicellular eukaryotes: causes and consequences. Nat. Rev. Genet.8: 619–631.10.1038/nrg2158Search in Google Scholar PubMed
Bakker, F.T., J.L. Olsen, W.T. Stam and C. van den Hoek. 1992. Nuclear rDNA internal transcribed spacer regions (ITS1 and ITS2) define discrete biogeographic groups in Cladophora albida (Chlorophyta). J. Phycol.28: 839–845.10.1111/j.0022-3646.1992.00839.xSearch in Google Scholar
Bakker, F.T., J.L. Olsen and W.T. Stam. 1995. Global phyloge-ography in the cosmopolitan species Cladophora vagabunda (Chlorophyta) based on nuclear rDNA internal transcribed spacer sequences. Eur. J. Phycol.30: 197–208.10.1080/09670269500650981Search in Google Scholar
Blair, S.M. 1983. Taxonomic treatment of the Chaetomorpha and Rhizoclonium species (Cladophorales; Chlorophyta) in New England. Rhodora85: 175–211.Search in Google Scholar
Blair, S.M., A.C. Mathieson and D.P. Cheney. 1982. Morphological and electrophoretic investigations of selected species of Chaetomorpha (Chlorophyta, Cladophorales). Phycologia21: 164–172.10.2216/i0031-8884-21-2-164.1Search in Google Scholar
Boedeker, C., A. Eggert, A. Immers and I. Wakana. 2010a. Biogeography of Aegagropilalinnaei (Cladophorophyceae, Chlorophyta): a widespread freshwater alga with low effective dispersal potential shows a glacial imprint in its distribution. J. Biogeogr.37: 1491–1503.10.1111/j.1365-2699.2010.02309.xSearch in Google Scholar
Boedeker, C., M.E. Ramírez and W.A. Nelson. 2010b. Cladophoropsis brachyartra from southern South America is a synonym of Wittrockiella lyallii (Cladophorophycaeae, Chlorophyta), previously regarded as endemic to New Zealand. Phycologia49: 525–536.10.2216/09-98.1Search in Google Scholar
Boedeker, C., F. Leliaert and G.C. Zuccarello. 2016. Molecular phylogeny of the Cladophoraceae (Cladophorales, Ulvophyceae), with the resurrection of Acrocladus Nägeli and Willeella Børgesen, and the description of Lurbica gen. nov. and Pseudorhizoclonium gen. nov. J. Phycol.52: 905–928.10.1111/jpy.12457Search in Google Scholar PubMed
Bromham, L. 2009. Why do species vary in their rate of molecular evolution? Biol. Lett.5: 401–404.10.1098/rsbl.2009.0136Search in Google Scholar PubMed PubMed Central
Burrows, E.M. 1991. Seaweeds of the British Isles. Vol. 2: Chlorophyta. Natural History Museum, London. pp. 238.Search in Google Scholar
Goff, L. and D. Moon. 1993. PCR amplification of nuclear and plastid genes from algal herbarium specimens and algal spores. J. Phycol.29: 381–384.10.1111/j.0022-3646.1993.00381.xSearch in Google Scholar
Guiry, M.D. and G.M. Guiry. 2016. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org; searched on 25 August 2016.Search in Google Scholar
Hassouna, N., B. Michot and J.P. Bachellerie. 1984. The complete nucleotide sequence of mouse 28S rRNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes. Nucleic Acids Res.12: 3563–3583.10.1093/nar/12.8.3563Search in Google Scholar PubMed PubMed Central
Hayakawa, Y.-I., T. Ogawa, S. Yoshikawa, K. Ohki and M. Kamiya. 2012. Genetic and ecophysiological diversity of Cladophora (Cladophorales, Ulvophyceae) in various salinity regimes. Phycol. Res.60: 86–97.10.1111/j.1440-1835.2012.00641.xSearch in Google Scholar
Kornmann, P. 1972. Ein Beitrag zur Taxonomie der Gattung Chaetomorpha (Cladophorales, Chlorophyta). Helgoländer Meeresunters.23: 1–31.10.1007/BF01616308Search in Google Scholar
Leliaert, F. and C. Boedeker. 2007. Cladophorales. In: (J. Brodie, C.A. Maggs and D. John, eds.) Green seaweeds of Britain and Ireland. Natural History Museum Publications, London. pp. 131–183.Search in Google Scholar
Leliaert, F., F. Rousseau, B. de Reviers and E. Coppejans. 2003. Phylogeny of the Cladophorophyceae (Chlorophyta) inferred from partial LSU rRNA gene sequences: is the recognition of a separate order Siphonocladales justified? Eur. J. Phycol.38: 233–246.10.1080/1364253031000136376Search in Google Scholar
Leliaert, F., B. Wysor, H. Verbruggen, C. Vlaeminck and O. De Clerck. 2008. Phyllodictyon robustum (Setchell et Gardner) comb. nov. (Siphonocladales, Chlorophyta), a morphologically variable species from the tropical Pacific coast of America. Crypt. Algol.29: 217–233.Search in Google Scholar
Leliaert, F., H. Verbruggen, B. Wysor and O. De Clerck. 2009. DNA taxonomy in morphologically plastic taxa: algorithmic species delimitation in the Boodlea complex (Chlorophyta: Cladophorales). Mol. Phylogen. Evol.53: 122–133.10.1016/j.ympev.2009.06.004Search in Google Scholar PubMed
Lindstrom, S.C. 1987. Possible sister groups and phylogenetic relationships among selected North Pacific and North Atlantic Rhodophyta. Helgoländer Meeresunters.41: 245–260.10.1007/BF02366190Search in Google Scholar
Lindstrom, S.C. 2001. The Bering Strait connection: dispersal and speciation in boreal macroalgae. J. Biogeogr.28: 243–251.10.1046/j.1365-2699.2001.00529.xSearch in Google Scholar
Lüning, K. 1985. Meeresbotanik. Thieme, Stuttgart. pp. 375.Search in Google Scholar
Marks, J.C. and M.P. Cummings. 1996. DNA sequence variation in the ribosomal internal transcribed spacer region of freshwater Cladophora species. J. Phycol.32: 1035–1042.10.1111/j.0022-3646.1996.01035.xSearch in Google Scholar
Moniz, M.B.J., M.D. Guiry and F. Rindi. 2014. tufA phylogeny and species boundaries in the green algal order Prasiolales (Trebouxiophyceae, Chlorophyta). Phycologia53: 396–406.10.2216/13-233.1Search in Google Scholar
Novaczek, I., G.W. Lubbers and A.M. Breeman. 1990. Thermal ecotypes of amphi-Atlantic algae. I. Algae of Arctic to cold-temperate distribution (Chaetomorphamelagonium, Devaleraea ramentacea and Phycodrys rubens). Helgoländer Meeresunters.44: 459–474.10.1007/BF02365480Search in Google Scholar
Peters, A.F., M.J.H. van Oppen, C. Wiencke, W.T. Stam and J.L. Olsen. 1997. Phylogeny and historical ecology of the Desmarestiaceae (Phaeophyceae) support a southern hemisphere origin. J. Phycol.33: 294–309.10.1111/j.0022-3646.1997.00294.xSearch in Google Scholar
Rambaut, A. 2007. Se-Al (multiple sequence alignment program), v2.0a11. Available at: http://tree.bio.ed.ac.uk/software/sea (last accessed 24 June 2016).Search in Google Scholar
Ross, S.J. 2006. Molecular phylogeography and species discrimination of freshwater Cladophora (Cladophorales, Chlorophyta) in North America. MSc Thesis, University of Waterloo, Canada, pp. 155. Available at: http://hdl.handle.net/10012/2977.Search in Google Scholar
Serrão, E.A., L.A. Alice and S.H. Brawley. 1999. Evolution of the Fucaceae (Phaeophyceae) inferred from nrDNA ITS. J. Phycol.35: 382–394.10.1046/j.1529-8817.1999.3520382.xSearch in Google Scholar
Silva, P.C. 1950. Generic names of algae proposed for conservation. Hydrobiologia2: 252–280.10.1007/BF00046559Search in Google Scholar
Silva, P.C., P.W. Basson and R.L. Moe. 1996. Catalogue of the benthic marine algae of the Indian Ocean. Univ. Calif. Publ. Bot.79: 1–1259.Search in Google Scholar
van den Hoek, C. 1984. World-wide latitudinal and longitudinal seaweed distribution patterns and their possible causes, as illustrated by the distribution of Rhodophytan genera. Helgoländer Meeresunters.38: 227–257.10.1007/BF01997483Search in Google Scholar
van den Hoek, C. and H.B.S. Womersley. 1984. Genus Cladophora. In: (H.B.S. Womersley, ed) The marine benthic flora of southern Australia. Part I. Government Printer, Adelaide. pp. 185–213.Search in Google Scholar
van Oppen, M.J.H., J.L. Olsen, W.T. Stam, C. van den Hoek and C. Wiencke. 1993. Arctic-Antarctic disjunctions in the benthic seaweeds Acrosiphonia arcta (Chlorophyta) and Desmarestia viridis/willii (Phaeophyta) are of recent origin. Mar. Biol.115: 381–386.10.1007/BF00349835Search in Google Scholar
van Oppen, M.J.H., S.G.A. Draisma, J.L. Olsen and W. Stam. 1995. Multiple trans-Arctic passages in the red alga Phycodrys rubens: evidence from nuclear rDNA ITS sequences. Mar. Biol.123: 179–188.10.1007/BF00350338Search in Google Scholar
White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal genes for phylogenetics PCR protocols. In: (M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White, eds) A guide to molecular methods and applications. Academic Press, New York. pp. 315–322.Search in Google Scholar
©2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and ecology
- Seasonal variations of Fucus vesiculosus fertility under ocean acidification and warming in the western Baltic Sea
- Recruitment in Ulva blooms in relation to temperature, salinity and nutrients in a subtropical bay of the Gulf of California
- In situ biomass production of Gracilariaceae and Ulva rigida: the Venice Lagoon as a study case
- Cystoseira scale-based biometric relationships
- Harvesting Ascophyllum nodosum (Phaeophyceae) reduces the abundance of its host-specific epiphyte Vertebrata lanosa (Rhodophyta)
- Taxonomy/phylogeny and biogeography
- Two new epizoic Achnanthes species (Bacillariophyta) living on marine turtles from Costa Rica
- Genetic diversity and biogeography in Chaetomorpha melagonium (Ulvophyceae, Cladophorales) based on internal transcribed spacer (ITS rDNA) sequences
- Genomics
- Cloning and application of the complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia haitanensis (Bangiales, Rhodophyta)
- Chemistry and applications
- Seasonal variation and yield of sulfated polysaccharides in seaweeds from the Venice Lagoon
- Degradation of dimethyl phthalate esters by a filamentous fungus Aspergillus versicolor isolated from deep-sea sediments
Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and ecology
- Seasonal variations of Fucus vesiculosus fertility under ocean acidification and warming in the western Baltic Sea
- Recruitment in Ulva blooms in relation to temperature, salinity and nutrients in a subtropical bay of the Gulf of California
- In situ biomass production of Gracilariaceae and Ulva rigida: the Venice Lagoon as a study case
- Cystoseira scale-based biometric relationships
- Harvesting Ascophyllum nodosum (Phaeophyceae) reduces the abundance of its host-specific epiphyte Vertebrata lanosa (Rhodophyta)
- Taxonomy/phylogeny and biogeography
- Two new epizoic Achnanthes species (Bacillariophyta) living on marine turtles from Costa Rica
- Genetic diversity and biogeography in Chaetomorpha melagonium (Ulvophyceae, Cladophorales) based on internal transcribed spacer (ITS rDNA) sequences
- Genomics
- Cloning and application of the complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia haitanensis (Bangiales, Rhodophyta)
- Chemistry and applications
- Seasonal variation and yield of sulfated polysaccharides in seaweeds from the Venice Lagoon
- Degradation of dimethyl phthalate esters by a filamentous fungus Aspergillus versicolor isolated from deep-sea sediments
