Fatty acid production of tropical thraustochytrids from Malaysian mangroves
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Mei-Cing Ou
Mei-Cing Ou obtained her BSc (Ecology and Biodiversity) from the University of Malaya, Malaysia. She is currently pursuing her MSc degree in the University of Malaya. Her research project is focused on the isolation, taxonomy and profiling of Malaysian thraustochytrids.
Hui-Yin Yeong obtained her BSc (Biomedical Technology) from the University of Malaya, Malaysia in 2002 and her PhD (Algal Biotechnology) from the same university in 2011. Since 2009, she has worked as a Research Officer at the Institute of Ocean and Earth Sciences (IOES), the University of Malaya where she developed tissue and protoplast culture of macroalgae. Her current interests include isolation and profiling of tropical thraustochytrids for application in nutraceuticals, cosmeceuticals and biofuel.
,
Ka-Lai Pang
Ka-Lai Pang obtained his BSc and PhD from the City University of Hong Kong in 1998 and 2001, respectively. During 2002–2007, he worked as a post-doc researcher at the University of Portsmouth (UK), BIOTEC (Thailand) and the City University of Hong Kong on various projects related to the ecology and phylogeny of marine fungi. In 2007, he joined the faculty at National Taiwan Ocean University where he studies the biology of marine fungi and fungus-like organisms, and endophytic fungi associated with mangrove plants and macroalgae.
Siew-Moi Phang obtained her PhD from the University of Malaya, Malaysia in Applied Phycology. She pioneered algal biotechnology research in Malaysia and established the Algae Research Laboratory at the University of Malaya. She has been the Director of the Institute of Ocean and Earth Sciences (IOES), the University of Malaya since 2008. She is a fellow of the Academy of Sciences Malaysia. She also serves as Assistant Editor for the
Journal of Applied Phycology .
Abstract
A total of 33 tropical thraustochytrid isolates were isolated from fallen leaves of Malaysian mangroves. Phylogenetic analysis based on the 18S rRNA gene revealed that the majority of the 33 isolates belonged to the genus Aurantiochytrium, and only one isolate belonged to Parietichytrium. The specific growth rate and biomass productivity of the strains ranged from 0.17 to 0.93 day−1 and 0.03 to 0.62 g l−1 day−1, respectively. Lipid productivity ranged from 1.27 to 70.86 mg l−1 day−1. Fifteen fatty acids, dominated by C16:0 [19.5%–71.6% total fatty acid (TFA)], C15:0 (3.3%–43.1% TFA), C22:6n3 (1.5%–38.4% TFA) and C18:0 (2.9%–28.6% TFA), were identified. The total carotenoid content was shown to be a better chemotaxonomic character than fatty acid composition for the isolates. Aurantiochytrium sp. UMACC-T024 had the highest lipid productivity (70.86 mg l−1 day−1), and this was selected for an optimisation study. The optimal conditions for lipid production of Aurantiochytrium sp. UMACC-T024 were salinity 32, pH 7.5, 1% w/v glucose and 0.5 g l−1 yeast extract, which resulted in an enhanced lipid productivity of 263.37 mg l−1 day−1 at day 5.
About the authors
Mei-Cing Ou obtained her BSc (Ecology and Biodiversity) from the University of Malaya, Malaysia. She is currently pursuing her MSc degree in the University of Malaya. Her research project is focused on the isolation, taxonomy and profiling of Malaysian thraustochytrids.
Hui-Yin Yeong obtained her BSc (Biomedical Technology) from the University of Malaya, Malaysia in 2002 and her PhD (Algal Biotechnology) from the same university in 2011. Since 2009, she has worked as a Research Officer at the Institute of Ocean and Earth Sciences (IOES), the University of Malaya where she developed tissue and protoplast culture of macroalgae. Her current interests include isolation and profiling of tropical thraustochytrids for application in nutraceuticals, cosmeceuticals and biofuel.
Ka-Lai Pang obtained his BSc and PhD from the City University of Hong Kong in 1998 and 2001, respectively. During 2002–2007, he worked as a post-doc researcher at the University of Portsmouth (UK), BIOTEC (Thailand) and the City University of Hong Kong on various projects related to the ecology and phylogeny of marine fungi. In 2007, he joined the faculty at National Taiwan Ocean University where he studies the biology of marine fungi and fungus-like organisms, and endophytic fungi associated with mangrove plants and macroalgae.
Siew-Moi Phang obtained her PhD from the University of Malaya, Malaysia in Applied Phycology. She pioneered algal biotechnology research in Malaysia and established the Algae Research Laboratory at the University of Malaya. She has been the Director of the Institute of Ocean and Earth Sciences (IOES), the University of Malaya since 2008. She is a fellow of the Academy of Sciences Malaysia. She also serves as Assistant Editor for the Journal of Applied Phycology.
Acknowledgements
Mei-Cing Ou specially thank Vejeysri Vello for her help in fatty acid analysis, and Sheng-Yu Guo from the National Taiwan Ocean University for phylogenetic analysis. This project was funded by the University of Malaya Research Grants (Grant nos.: RP001J-13SUS and RP001O-13SUS).
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Articles in the same Issue
- Frontmatter
- In this issue
- Research articles
- Fungal diversity of marine biofilms on artificial reefs in the north-central Gulf of Mexico
- Genetic diversity and population structure of Corollospora maritima sensu lato: new insights from population genetics
- Fatty acid production of tropical thraustochytrids from Malaysian mangroves
- A new genus Phyllophorella gen. nov. (Phyllophoraceae, Rhodophyta) from central Peru, including Phyllophorella peruviana comb. nov., Phyllophorella humboldtiana sp. nov., and Phyllophorella limaensis sp. nov.
- Morphology and phylogeny of Pterosiphonia dendroidea (Rhodomelaceae, Ceramiales) described as Pterosiphonia tanakae from Japan
- Cryptic diversity of the mangrove-associated alga Bostrychia (Rhodomelaceae, Rhodophyta) from Thailand
- Clump structure, population structure and non-destructive biomass estimation of the New Zealand carrageenophyte Sarcothalia lanceata (Gigartinaceae, Rhodophyta)
- Desiccation tolerance and underlying mechanisms for the recovery of the photosynthetic efficiency in the tropical intertidal seagrasses Halophila ovalis and Thalassia hemprichii
Articles in the same Issue
- Frontmatter
- In this issue
- Research articles
- Fungal diversity of marine biofilms on artificial reefs in the north-central Gulf of Mexico
- Genetic diversity and population structure of Corollospora maritima sensu lato: new insights from population genetics
- Fatty acid production of tropical thraustochytrids from Malaysian mangroves
- A new genus Phyllophorella gen. nov. (Phyllophoraceae, Rhodophyta) from central Peru, including Phyllophorella peruviana comb. nov., Phyllophorella humboldtiana sp. nov., and Phyllophorella limaensis sp. nov.
- Morphology and phylogeny of Pterosiphonia dendroidea (Rhodomelaceae, Ceramiales) described as Pterosiphonia tanakae from Japan
- Cryptic diversity of the mangrove-associated alga Bostrychia (Rhodomelaceae, Rhodophyta) from Thailand
- Clump structure, population structure and non-destructive biomass estimation of the New Zealand carrageenophyte Sarcothalia lanceata (Gigartinaceae, Rhodophyta)
- Desiccation tolerance and underlying mechanisms for the recovery of the photosynthetic efficiency in the tropical intertidal seagrasses Halophila ovalis and Thalassia hemprichii
