Abstract
Manganese superoxide dismutase (Mn-SOD) is an essential enzyme involved in the detoxification of reactive oxygen by dismutation of the superoxide radical anion. In this study, full-length complementary DNA (cDNA) and genomic DNA of Mn-sod genes were cloned from Pyropia haitanensis (phsod), and its expression was monitored using quantitative polymerase chain reaction (qPCR). Scavenging of reactive oxygen species under heat shock was analyzed as well. The full-length cDNA of the phsod gene consists of 935 bp (GenBank accession number: EU715988) with an open reading frame of 675 bp, which encodes 224 amino acids. The length of the genomic sequence is 1416 bp and includes four exons and three introns. A highly conserved Mn-SOD signature (DVWEHAYY) was found. The transit peptide in the N-terminal helps protein targeting to the mitochondria. Results showed that the expression levels of phsod increased 3.4 times compared to controls and then suddenly decreased under a heat shock of 35°C for 30 min. After heat shock exposure and return to normal cultivation temperature, the expression levels of phsod decreased within 1 h of release from the heat shock and then increased within 3 h. SOD activity correlated with the expression of the phsod gene and, as expected, the concentration of ·O2- showed negative correlations with SOD activity.
This research was supported by the National Natural Science Foundation of China No. 40776077, Natural Science Foundation of Zhejiang No. LY12D06003, National Scientific and Technological Support Plan Subject, 2011BAD13B08, Zhejiang Scientific Innovative Team No. 2012R10025-07 and No.2011R09012-06, Ningbo Marine Algae Biotechnology Team No. 2011B81007 and No. 2012A610140 and the Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences. We thank Prof. Matthew J. Dring, Prof. Kirsten Heimann and Dr. Gunda Stöber for their assistance in editing this paper.
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©2013 by Walter de Gruyter Berlin Boston
Articles in the same Issue
- Masthead
- Masthead
- Research articles
- Checklist of the marine macroalgae of Vietnam
- A reassessment of the foliose Bangiales (Rhodophyta) in the Balearic Islands including the proposed synonymy of Pyropia olivii with Pyropia koreana
- Intraspecific variation in Gracilaria birdiae (Gracilariales, Rhodophyta): growth, and agar yield and quality of color strains under aquaculture
- Sequences of Mn-sod gene from Pyropia haitanensis (Bangiales, Rhodophyta) and its expression under heat shock
- Variation in pigment content of Thalassia testudinum seedlings in response to changes in salinity and light
- An allometric method for measuring leaf growth in eelgrass, Zostera marina, using leaf length data
- Isolation and bioassay screening of fungal endophytes from North Atlantic marine macroalgae
- Short communication
- New records of three dictyotalean brown algae for Turkey
Articles in the same Issue
- Masthead
- Masthead
- Research articles
- Checklist of the marine macroalgae of Vietnam
- A reassessment of the foliose Bangiales (Rhodophyta) in the Balearic Islands including the proposed synonymy of Pyropia olivii with Pyropia koreana
- Intraspecific variation in Gracilaria birdiae (Gracilariales, Rhodophyta): growth, and agar yield and quality of color strains under aquaculture
- Sequences of Mn-sod gene from Pyropia haitanensis (Bangiales, Rhodophyta) and its expression under heat shock
- Variation in pigment content of Thalassia testudinum seedlings in response to changes in salinity and light
- An allometric method for measuring leaf growth in eelgrass, Zostera marina, using leaf length data
- Isolation and bioassay screening of fungal endophytes from North Atlantic marine macroalgae
- Short communication
- New records of three dictyotalean brown algae for Turkey