Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditionsa
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Abstract
To characterize the structure and function of the protein phosphatase 2C (PP2C) gene in Porphyra yezoensis, full-length cDNA of the locus (denoted pypp2c) was obtained by electronic cloning, rapid amplification of cDNA ends, and reverse-transcription PCR. The nucleotide sequence of PyPP2C was 1988 bp, including a 5′-untranslated region (UTR) of 301 bp, a 3′-UTR of 256 bp, and an open reading frame of 1431 bp that can be translated into 476 amino acids with a molecular mass of 50.96 kDa and a putative isoelectric point of 8.34. The amino acids had 30–36% identity with those encoded by PP2C genes in protozoa and higher plants. A PP2C-like catalytic domain was found in the 57–397 region, and several residues involved in the metal ion binding sites were conserved. mRNA expression levels in the three life stages of leafy thalli, filaments, and conchospores were examined by real-time fluorescent quantitative PCR. Highest expression of pypp2c occurred in conchospores. Gene expression levels were similar among the three life stages. pypp2c expression at different thallus relative water contents (RWCs) varied, with the highest expression occurring at 60% RWC.
©2012 by Walter de Gruyter Berlin Boston
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Articles in the same Issue
- Masthead
- Masthead
- Research Articles
- Polysiphonia sensu lato (Ceramiales, Florideophyceae) species of Caribbean Panama including Polysiphonia lobophoralis sp. nov. and Polysiphonia nuda sp. nov.
- Molecular support for the establishment of the new genus Laurenciella within the Laurencia complex (Ceramiales, Rhodophyta)
- Neosiphonia peruviensis sp. nov. (Rhodomelaceae, Rhodophyta) from the Pacific coast of South America
- Colpomenia durvillei (Scytosiphonaceae, Phaeophyceae): its distribution and relationships with other elongate species of the genus
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- Diversity, occurrence, and habitats of the diatom genus Bacteriastrum (Bacillariophyta) in the northern Adriatic Sea, with the description of B. jadranum sp. nov.
- Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditionsa
- Antibacterial compound from the endophytic fungus Phomopsis longicolla isolated from the tropical red seaweed Bostrychia radicans
- Short Communication
- First record of fertile gametophytes of Polysiphonia perforans (Ceramiales, Rhodophyta)
