Osteogenic capacity of transgenic flax scaffolds
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Tomasz Gredes
,
Magdalena Wróbel-Kwiatkowska
Abstract
The modification of flax fibers to create biologically active dressings is of undoubted scientific and practical interest. Flax fibers, derived from transgenic flax expressing three bacterial genes for the synthesis of poly-3-hydroxybutyric acid (PHB), have better mechanical properties than unmodified flax fibers; do not show any inflammation response after subcutaneous insertion; and have a good in vitro and in vivo biocompatibility. The aim of this study was to examine the applicability of composites containing flax fibers of genetically modified (M50) or non-modified (wt-Nike) flax within a polylactide (PLA) matrix for bone regeneration. For this, the mRNA expression of genes coding for growth factors (insulin-like growth factor IGF1, IGF2, vascular endothelial growth factor), for osteogenic differentiation (alkaline phosphatase, osteocalcin, Runx2, Phex, type 1 and type 2 collagen), and for bone resorption markers [matrix metalloproteinase 8 (MMP8), acid phosphatase type 5] were analyzed using quantitative real-time polymerase chain reaction. We found a significant elevated mRNA expression of IGF1 with PLA and PLA-wt-Nike composites. The mRNA amount of MMP8 and osteocalcin was significantly decreased in all biocomposite-treated cranial tissue samples compared to controls, whereas the expression of all other tested transcripts did not show any differences. It is assumed that both flax composites are able to stimulate bone regeneration, but composites from transgenic flax plants producing PHB showed faster bone regeneration than composites of non-transgenic flax plants. The application of these linen membranes for bone tissue engineering should be proved in further studies.
©2012 by Walter de Gruyter Berlin Boston
Artikel in diesem Heft
- Guest editorial
- 10.1515/bmt-2012-0099
- Original Articles
- Immediate loading of an implant with fine threaded neck – bone resorption and clinical outcome of single tooth restorations in the maxilla
- The survival and proliferation of fibroblasts on ceramic implants: an in vitro study
- Simulating the trabecular bone structure around dental implants: a case presentation
- Biomechanical finite element analysis of small diameter and short dental implants: extensive study of commercial implants
- Bone loss of immediately loaded implants with implant-supported and tooth-implant-supported fixed maxillary prostheses
- Retrievable, tooth-implant-supported, complete-arch fixed restorations in the maxilla: a 6-year retrospective study
- Crestal minimally-invasive sinus lift on severely resorbed maxillary crest: prospective study
- Osteogenic capacity of transgenic flax scaffolds
- Detection of surfactant proteins A, B, C, and D in human gingiva and saliva
- Orofacial characteristics of adolescents with diagnosed spinal disorders
- 3D assisted morphological analysis of lingual upper central and lateral incisor surfaces
- Prelims
- Prelims
Artikel in diesem Heft
- Guest editorial
- 10.1515/bmt-2012-0099
- Original Articles
- Immediate loading of an implant with fine threaded neck – bone resorption and clinical outcome of single tooth restorations in the maxilla
- The survival and proliferation of fibroblasts on ceramic implants: an in vitro study
- Simulating the trabecular bone structure around dental implants: a case presentation
- Biomechanical finite element analysis of small diameter and short dental implants: extensive study of commercial implants
- Bone loss of immediately loaded implants with implant-supported and tooth-implant-supported fixed maxillary prostheses
- Retrievable, tooth-implant-supported, complete-arch fixed restorations in the maxilla: a 6-year retrospective study
- Crestal minimally-invasive sinus lift on severely resorbed maxillary crest: prospective study
- Osteogenic capacity of transgenic flax scaffolds
- Detection of surfactant proteins A, B, C, and D in human gingiva and saliva
- Orofacial characteristics of adolescents with diagnosed spinal disorders
- 3D assisted morphological analysis of lingual upper central and lateral incisor surfaces
- Prelims
- Prelims
