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TK1656, an L-asparaginase from Thermococcus kodakarensis, a novel candidate for therapeutic applications

  • Shahid Mahmood Chohan , Muhammad Atif Nisar , Naeem Rashid EMAIL logo , Ghazaleh Gharib , Qamar Bashir and Masood Ahmed Siddiqui
Published/Copyright: January 11, 2017
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Biologia
From the journal Biologia Volume 71 Issue 12

Abstract

L-Asparaginase is a potential therapeutic agent owing to its anti-tumor activity. We have previously characterized a thermostable L-asparaginase (TK1656F from Thermococcus kodakaraensis that exhibits highest ever reported enzymatic activity, no glutaminase activity and high stability. In this study we have compared the cytotoxicity and anti-proliferative activity of TK1656 and a commercially available L-asparaginase from Escherichia coli on cancerous cells. Despite of the thermophilic origin, the amount of TK1656 required to inhibit equivalent growth of HeLa cells at 37°C was three times less than that of the L-asparaginase from E. coli. It is worth mentioning that TK1656 showed much reduced cytotoxic effect on non-cancerous cells as compared to the E. coli counterpart. Furthermore, TK1656 was found to potentiate induction of apoptosis of cancerous cells. Overall, our findings suggest that TK1656 harbors a remarkable potential for the cancer chemotherapeutics.

Key words: thermostable L-asparaginase; Thermococcus kodakaraensis; therapeutic agent; cytotoxicity; anti-proliferative
* The first two authors contributed equally to this work and, in their opinion, should be considered as joint first author.
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Received: 2016-11-8
Accepted: 2016-12-12
Published Online: 2017-1-11
Published in Print: 2016-12-1

© 2016 Institute of Molecular Biology, Slovak Academy of Sciences

Articles in the same Issue

  1. Cellular and Molecular Biology
  2. TK1656, an L-asparaginase from Thermococcus kodakarensis, a novel candidate for therapeutic applications
  3. Botany
  4. Dynamic change of the rhizosphere microbial community in response to growth stages of consecutively monocultured Rehmanniae glutinosa
  5. Botany
  6. Comparative root and stem anatomy of six Clinopodium (Lamiaceae) taxa
  7. Botany
  8. The alleviating effects of salicylic acid application against aluminium toxicity in barley (Hordeum vulgare) roots
  9. Botany
  10. Recovery capacity of the edible halophyte Crithmum maritimum from temporary salinity in relation to nutrient accumulation and nitrogen metabolism
  11. Zoology
  12. Reproductive strategy in rock-dwelling snail Cochlodina orthostoma (Gastropoda: Pulmonata: Clausiliidae)
  13. Zoology
  14. Meteorite crater ponds as source of high zooplankton biodiversity
  15. Zoology
  16. Diel activity and use of multiple artificially constructed shelters in Astacus leptodactylus (Decapoda: Astacidae)
  17. Zoology
  18. Predictions of marbled crayfish establishment in conurbations fulfilled: Evidences from the Czech Republic
  19. Zoology
  20. High genetic diversity and a new cryptic species within the Ephedrus persicae species group (Hymenoptera: Braconidae: Aphidiinae)
  21. Zoology
  22. Analysis of δ13C and δ15N isotopic signatures to shed light on the hydrological cycle’s influence on the trophic behavior of fish in a Mediterranean reservoir
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https://doi.org/10.1515/biolog-2016-0168
Keywords for this article
thermostable L-asparaginase; Thermococcus kodakaraensis; therapeutic agent; cytotoxicity; anti-proliferative

Articles in the same Issue

  1. Cellular and Molecular Biology
  2. TK1656, an L-asparaginase from Thermococcus kodakarensis, a novel candidate for therapeutic applications
  3. Botany
  4. Dynamic change of the rhizosphere microbial community in response to growth stages of consecutively monocultured Rehmanniae glutinosa
  5. Botany
  6. Comparative root and stem anatomy of six Clinopodium (Lamiaceae) taxa
  7. Botany
  8. The alleviating effects of salicylic acid application against aluminium toxicity in barley (Hordeum vulgare) roots
  9. Botany
  10. Recovery capacity of the edible halophyte Crithmum maritimum from temporary salinity in relation to nutrient accumulation and nitrogen metabolism
  11. Zoology
  12. Reproductive strategy in rock-dwelling snail Cochlodina orthostoma (Gastropoda: Pulmonata: Clausiliidae)
  13. Zoology
  14. Meteorite crater ponds as source of high zooplankton biodiversity
  15. Zoology
  16. Diel activity and use of multiple artificially constructed shelters in Astacus leptodactylus (Decapoda: Astacidae)
  17. Zoology
  18. Predictions of marbled crayfish establishment in conurbations fulfilled: Evidences from the Czech Republic
  19. Zoology
  20. High genetic diversity and a new cryptic species within the Ephedrus persicae species group (Hymenoptera: Braconidae: Aphidiinae)
  21. Zoology
  22. Analysis of δ13C and δ15N isotopic signatures to shed light on the hydrological cycle’s influence on the trophic behavior of fish in a Mediterranean reservoir
  23. Cellular and Molecular Biology
  24. Role of FIT2 in porcine intramuscular preadipocyte differentiation
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