Down into the Earth: microbial diversity of the deepest cave of the world
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Ieva Kieraite-Aleksandrova
Abstract
In our work, microbial diversity of Krubera-Voronja cave was evaluated in the view of the frequency of human visits in different locations as well as the sampling depth. Sampling in this cave was performed at depths of 220 m to 1640 m. Cultivation-independent method, namely barcoded pyrosequencing of 16S rRNA gene, was used for this analysis. Our results demonstrated high bacterial diversity at the phylum and genus levels. We have shown that the bacterial diversity at the phylum level depends on both the sampling depth and the frequency of human visits in Krubera-Voronja cave. Frequently visited locations were more diverse at the phylum level than the rarely visited branches. The total number of bacterial genera both per phylum and per sample correlated with the frequency of human visits but not with the sampling depth. Some genera, found in Krubera-Voronja cave, seem to be absent or very rare in other caves. The present study represents the first report on the microbial diversity in Krubera-Voronja cave
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Articles in the same Issue
- Down into the Earth: microbial diversity of the deepest cave of the world
- Factors influencing synergistic antimicrobial activity of thymol and nisin against Shigella spp. in sugarcane juice
- Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans
- Mitochondrial structures during seed germination and early seedling development in Arabidopsis thaliana
- Transcriptome analysis for identification of indigo biosynthesis pathway genes in Polygonum tinctorium
- Different components of plant diversity suggest the protection of a large area for the conservation of a riparian ecosystem
- Anatomical adaptations of the desert species Stipa lagascae against drought stress
- Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma
- cDNA cloning, heterologous expression and characterization of a cell wall invertase from copper tolerant population of Elsholtzia haichowensis
- Construction of cDNA library from Prunus campanulata leaves and preliminary expressed sequence tag (EST) analysis during cold stress
- Phylogenetic utility of the geometric model of the body form in leeches (Clitellata: Hirudinida)
- Substrate choice by the alien snail Ferrissia fragilis (Gastropoda: Planorbidae) in an industrial area: A case study in a forest pond (Southern Poland)
- History of two critically endangered grassland snails (Pulmonata: Helicellinae) in the Czech Republic with first molecular data on extinct populations
- Monteustium marezensis gen. n., sp. n. and the first record of Italustiun eframi (Acari: Prostigmata: Erythraeidae: Balaustiinae) from Montenegro
- Lithobius (Ezembius) laevidentata sp. n., a new species (Chilopoda: Lithobiomorpha: Lithobiidae) from the Northwest region of China
- Aphids in jeopardy? Aphid communities on xerothermic habitats
- Habitat and weather requirements of diurnal raptors wintering in river valleys
