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Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation
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Anne Gauthier
Veröffentlicht/Copyright:
6. April 2010
Abstract
Alzheimer's disease and other neurodegenerative disorders share some common features at the cellular level, which are often associated with a change in cytoskeletal dynamics. Live cell imaging has been applied to study various aspects of cell physiology including cytoskeletal dynamics. Recently, fluorescence photoactivation (FPA) has been developed as a novel approach to analyze protein dynamics in living cells with high sensitivity. Here, we describe the application of FPA to determine interactions and mobility of the microtubule-associated protein tau in neurons as an example for a disease-related cytoskeletal protein and discuss further applications of FPA to study cytoskeletal dynamics in neurodegenerative diseases.
Keywords: Alzheimer's disease; microtubule-associated protein; neurodegeneration; photoactivatable GFP; photoactivation; tau protein
Received: 2009-11-29
Accepted: 2010-1-20
Published Online: 2010-04-06
Published in Print: 2010-06-01
©2010 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
Alzheimer's disease;
microtubule-associated protein;
neurodegeneration;
photoactivatable GFP;
photoactivation;
tau protein
Artikel in diesem Heft
- Guest Editorial
- Highlight: Molecular Neurobiology
- HIGHLIGHT: MOLECULAR NEUROBIOLOGY
- Actin-mediated gene expression in neurons: the MRTF-SRF connection
- RIM proteins and their role in synapse function
- Brain tumor stem cells
- The mitochondria permeability transition pore complex in the brain with interacting proteins – promising targets for protection in neurodegenerative diseases
- Involvement of the calcium sensor GCAP1 in hereditary cone dystrophies
- Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation
- REVIEWS
- Contributions of the Ah receptor to bilirubin homeostasis and its antioxidative and atheroprotective functions
- Cathepsin L in metastatic bone disease: therapeutic implications
- GENES AND NUCLEIC ACIDS
- Activity-based selection of HIV-1 reverse transcriptase variants with decreased polymerization fidelity
- CELL BIOLOGY AND SIGNALING
- Multiple protective functions of catalase against intercellular apoptosis-inducing ROS signaling of human tumor cells
- Molecular characterisation of ‘transmembrane protein 192’ (TMEM192), a novel protein of the lysosomal membrane
- PROTEOLYSIS
- Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases
