Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis
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Urška Požgan
Abstract
Cysteine cathepsins and matrix metalloproteases are considered to play important roles in the development of arthritic diseases. Their accumulation in synovial fluid of primarily rheumatoid arthritis patients is also well documented. However, a detailed comparison between the protease levels and activities between rheumatoid arthritis samples and osteoarthritis samples has never been made. Here, we report that both cysteine cathepsins B and S and matrix metalloproteases-1, -3 and -13 are detected in patient synovial fluid samples with significantly higher levels detected in rheumatoid arthritis patients. Among the proteases, cathepsin S was found to be significantly elevated, consistent with its critical role in the immune response. These results suggest that cysteine cathepsins have a major role in inflammation at least in rheumatoid arthritis. In addition to proteases, interleukin-6 was detected at significant levels in most samples, suggesting that proinflammatory cytokines might be in-volved in the stimulation of expression of these proteases during inflammation.
©2010 by Walter de Gruyter Berlin New York
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Artikel in diesem Heft
- REVIEW
- Chaperone-assisted degradation: multiple paths to destruction
- MINIREVIEWS
- Principles, implementation, and application of biology-oriented synthesis (BIOS)
- The molecular biology of moenomycins: towards novel antibiotics based on inhibition of bacterial peptidoglycan glycosyltransferases
- Kallikrein-related peptidase genes as promising biomarkers for prognosis and monitoring of human malignancies
- GENES AND NUCLEIC ACIDS
- Zinc supplement greatly improves the condition of parkin mutant Drosophila
- MOLECULAR MEDICINE
- Differential role of cathepsins B and L in autophagy-associated cell death induced by arsenic trioxide in U87 human glioblastoma cells
- CELL BIOLOGY AND SIGNALING
- Role of N-acetyl-N-nitroso-tryptophan as nitric oxide donor in the modulation of HIF-1-dependent signaling
- The role of salivary histatin and the human cathelicidin LL-37 in wound healing and innate immunity
- PROTEOLYSIS
- Detection and in-cell selectivity profiling of the full-length West Nile virus NS2B/NS3 serine protease using membrane-anchored fluorescent substrates
- Plasminogen hydrolysis by cathepsin S and identification of derived peptides as selective substrate for cathepsin V and cathepsin L inhibitor
- Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis
- Interdependence of kallikrein-related peptidases in proteolytic networks
