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Characterization of phosphate transporter(s) and understanding their role in Leishmania donovani parasite

  • K.J. Sindhu , Amit Kumar Kureel , Sheetal Saini , Smita Kumari , Pankaj Verma and Ambak Kumar Rai EMAIL logo
Published/Copyright: January 17, 2018
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Acta Parasitologica
From the journal Acta Parasitologica Volume 63 Issue 1

Abstract

Inorganic phosphate (Pi) is shown to be involved in excretion of methylglyoxal (MG) in the promastigote form of Leishmania donovani parasite. Absence of Pi leads to its accumulation inside the parasite. Accumulation of MG is toxic to the parasite and utilizes glyoxylase as well as excretory pathways for its detoxification. In addition, Pi is also reported to regulate activities of ectoenzymes and energy metabolism (glucose to pyruvate) etc. Thus, it is known to cumulatively affect the growth of Leishmania parasite. Hence the transporters, which allow the movement of Pi across the membrane, can prove to be a crucial drug target. Therefore, we characterized two phosphate transporters in Leishmania (i) H+ dependent myo-inositol transporter (LdPHO84), and (ii) Na+ dependent transporter (LdPHO89), based on similar studies done previously on other lower organisms and trypanosomatids. We tried to understand the secondary structure of these two proteins and confirm modulation in their expression with the change in Pi concentration outside. Moreover, their modes of action were also measured in the presence of specific inhibitors (LiF, CCCP). Further analysis on the physiological role of these transporters in various stages of the parasite life cycle needs to be entrenched.

Keywords: Leishmania; inorganic phosphate; Pi transporters; methylglyoxal; drug resistance

  1. Conflicts of interest: The authors declare no conflict of interest.

Acknowledgement

The authors thank all support received from Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad. We are thankful to Dr. Anuradha Dube and Dr. Amogh Sahtrabuddhe (Central Drug Research Institute, Lucknow, India) for providing promastigote culture of Leishmania donovani. We also acknowledge Centre for Medical Diagnostics and Research (CMDR) for allowing us to use real time PCR facility. This work is carried out as a part of dissertation of Ms. Sindhu K. J. in the fulfilment of her M. Tech. degree.

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Received: 2017-6-16
Revised: 2017-10-7
Accepted: 2017-10-12
Published Online: 2018-1-17
Published in Print: 2018-3-26

© 2018 W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2018-0009
Keywords for this article
Leishmania; inorganic phosphate; Pi transporters; methylglyoxal; drug resistance

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  4. Phylogeny and cocoon production in the parasitic leech Myzobdella lugubris Leidy, 1851 (Hirudinidae, Piscicolidae)
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  16. Scanning electron microscopy study of Strabax monstrosus von Nordmann, 1864 (Copepoda: Chondracanthidae) from Tunisian waters and histopathological study of its effects on host
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  20. Two nematode species from freshwater and marine fishes in Thailand, including Ascarophis scatophagi sp. nov. (Cystidicolidae) from Scatophagus argus (Scatophagidae)
  22. Metazoan parasite fauna of migrating common garfish, Belone belone (L.), in the Baltic Sea
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  26. Asymmetric peptidomimetics containing L-tartaric acid core inhibit the aspartyl peptidase activity and growth of Leishmania amazonensis promastigotes
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  32. Contamination of swimming pools and hot tubs biofilms with Acanthamoeba
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  36. Nematode Thelazia callipaeda is spreading across Europe. The first survey of red foxes from Slovakia
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  48. The first case of Leporacarus gibbus infestation in a rabbit from Poland
  49. Research Note
  50. Pathological and molecular studies of the renal trematode Paratanaisia bragai in Indian peafowls (Pavo cristatus)
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