Molecular and morphological characterisation of Rhabdias picardiae Junker, Lhermitte-Vallarino et Bain, 2010 (Nematoda: Rhabdiasidae) from Delaland’s River Frog, Amietia delalandii (Duméril et Bibron, 1841) (Amphibia: Pyxicephalidae) in South Africa
-
Roman Svitin
,
Yuriy Kuzmin
Abstract
Rhabdias picardiae previously known from the Guttural Toad, Sclerophrys gutturalis (Bufonidae) is described from a new host, Delaland’s River Frog, Amietia delalandii (Pyxicephalidae). The species identification is confirmed by analysis of the COI gene sequence and morphological comparison with the original description. Morphological details of apical structures in adult parasitic R. picardiae, namely, the shape and position of lips, the number and position of external labial papillae, and the buccal capsule morphology are described using light and scanning electron microscopy. The buccal capsule width and length, the distance from anterior end to nerve ring and excretory pore, and ratios of distance to vulva to body length and body length to width were identified as less variable metrical characters in the studied sample of 30 specimens. Adult specimens demonstrated differences in the position of lips in relation to the oral opening and position of the buccal capsule in relation to the oesophagus depending on the size (age) of worms. The infective larvae of R. picardiae are described for the first time and characterised by two lateral alae consisting of two ridges, triangular pseudolabia with rounded tops, and presence of ornamentation on the rounded tail tip.
Acknowledgements
The authors wish to express their thanks to Mr. Willie Landman for his technical assistance in SEM studies. Light microscopy was performed using the equipment of the Centre of Collective Use of Scientific Equipment “Animalia” (I. I. Schmalhausen Institute of Zoology, NAS of Ukraine).
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© 2018 W. Stefański Institute of Parasitology, PAS
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Articles in the same Issue
- Invited Paper
- Ultrastructure of digenean trematode eggs (Platyhelminthes: Neoophora): A review emphasizing new comparative data on four European Microphalloidea
- Phylogeny and cocoon production in the parasitic leech Myzobdella lugubris Leidy, 1851 (Hirudinidae, Piscicolidae)
- Parasites in stray dogs from Italy: prevalence, risk factors and management concerns
- A serological study of antibodies to Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in the sera of healthy individuals collected two decades apart
- Changes in the incidence of intestinal giardiosis in Mexican population during five years (2011-2015)
- Molecular target analysis of stearoyl-CoA desaturase genes of protozoan parasites
- Molecular and morphological characterisation of Rhabdias picardiae Junker, Lhermitte-Vallarino et Bain, 2010 (Nematoda: Rhabdiasidae) from Delaland’s River Frog, Amietia delalandii (Duméril et Bibron, 1841) (Amphibia: Pyxicephalidae) in South Africa
- Scanning electron microscopy study of Strabax monstrosus von Nordmann, 1864 (Copepoda: Chondracanthidae) from Tunisian waters and histopathological study of its effects on host
- Characterization of phosphate transporter(s) and understanding their role in Leishmania donovani parasite
- Two nematode species from freshwater and marine fishes in Thailand, including Ascarophis scatophagi sp. nov. (Cystidicolidae) from Scatophagus argus (Scatophagidae)
- Metazoan parasite fauna of migrating common garfish, Belone belone (L.), in the Baltic Sea
- Measurement of binding strength between prey proteins interacting with Toxoplasma gondii SAG1 and SAG2 using isothermal titration calorimetry (ITC)
- Asymmetric peptidomimetics containing L-tartaric acid core inhibit the aspartyl peptidase activity and growth of Leishmania amazonensis promastigotes
- Transcriptional analysis of immune-relevant genes in the mucus of Labeo rohita, experimentally infected with Argulus siamensis
- Prevalence of endoparasitic infections of birds in zoo gardens in Serbia
- Contamination of swimming pools and hot tubs biofilms with Acanthamoeba
- Morphological and molecular characterization of Seuratascaris numidica (Seurat, 1917) (Ascaridida: Ascarididae)
- Nematode Thelazia callipaeda is spreading across Europe. The first survey of red foxes from Slovakia
- A frequent roundworm Baylisascaris transfuga in overpopulated brown bears (Ursus arctos) in Slovakia: a problem worthy of attention
- Nematode parasites of Duttaphrynus stomaticus (Lutken, 1864) (Amphibia: Anura) with description of a new species of Rhabdias Stiles and Hassall, 1905 (Nematoda: Rhabdiasidae) from Dehradun (Uttarakhand), India
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