Abstract
Aphelenchoides besseyi, the nematode agent of rice tip white disease, causes huge economic losses in almost all the rice-growing regions of the world. Glutathione peroxidase (GPx), an esophageal glands secretion protein, plays important roles in the parasitism, immune evasion, reproduction and pathogenesis of many plant-parasitic nematodes (PPNs). Therefore, GPx is a promising target for control A. besseyi. Here, the full-length sequence of the GPx gene from A. besseyi (AbGPx1) was cloned using the rapid amplification of cDNA ends method. The full-length 944 bp AbGPx1 sequence, which contains a 678 bp open reading frame, encodes a 225 amino acid protein. The deduced amino acid sequence of the AbGPxl shares highly homologous with other nematode GPxs, and showed the closest evolutionary relationship with DrGPx. In situ hybridization showed that AbGPx1 was constitutively expressed in the esophageal glands of A. besseyi, suggesting its potential roles in parasitism and reproduction. RNA interference (RNAi) was used to assess the functions of the AbGPx1 gene, and quantitative real-time PCR was used to monitor the RNAi effects. After treatment with dsRNA for 12 h, AbGPx1 expression levels and reproduction in the nematodes decreased compared with the same parameters in the control group; thus, the AbGPx1 gene is likely to be associated with the development, reproduction, and infection ability of A. besseyi. These findings may open new avenues towards nematode control.
Acknowledgments
This work was supported by the Special Fund for Forest-Scientific Research in the Public Interest (Grant No. 201304403-4), the Heilongjiang Province Natural Science Foundation of China (Grant NO. ZD201404) and the Fundamental Research Funds for the Central Universities ( Grant NO. 2572015AA09).
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© 2017 W. Stefański Institute of Parasitology, PAS
Articles in the same Issue
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- Snail species diversity impacts the infection patterns of Echinostoma spp.: Examples from field collected data
- Molecular phylogeny of the Haplosplanchnata Olson, Cribb, Tkach, Bray and Littlewood, 2003, with a description of Schikhobalotrema huffmani n. sp.
- Structuraland antigenic analysis of a new Rhoptry Pseudokinase Gene (ROP54) in Toxoplasma gondii
- Ultrastructure of the spermatozoon of Macvicaria obovata (Digenea: Opecoelidae), a parasite of Sparus aurata(Pisces: Teleostei) from the Gulf of Gabès, Mediterranean Sea
- Epidemiology of Toxoplasma gondii infection in native Tibetans in Tibet, China
- A PCR survey of vector-borne pathogens in different dog populations from Turkey
- Diplectanids (Monogenea) parasitic on sciaenid fish from Peru with the proposal of Pseudorhamnocercoides n. gen., the description of Rhamnocercus dominguesi n. sp. and the redescription of Rhamnocercoides menticirrhi Luque and Iannacone, 1991
- Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions
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- Seroprevalence and risk factors of Neospora spp. and Toxoplasma gondii infections among horses and donkeys in Nigeria, West Africa
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- Erratum to: 2017, 62(1) should be 2017, 62(2)
Articles in the same Issue
- Review
- Snail species diversity impacts the infection patterns of Echinostoma spp.: Examples from field collected data
- Molecular phylogeny of the Haplosplanchnata Olson, Cribb, Tkach, Bray and Littlewood, 2003, with a description of Schikhobalotrema huffmani n. sp.
- Structuraland antigenic analysis of a new Rhoptry Pseudokinase Gene (ROP54) in Toxoplasma gondii
- Ultrastructure of the spermatozoon of Macvicaria obovata (Digenea: Opecoelidae), a parasite of Sparus aurata(Pisces: Teleostei) from the Gulf of Gabès, Mediterranean Sea
- Epidemiology of Toxoplasma gondii infection in native Tibetans in Tibet, China
- A PCR survey of vector-borne pathogens in different dog populations from Turkey
- Diplectanids (Monogenea) parasitic on sciaenid fish from Peru with the proposal of Pseudorhamnocercoides n. gen., the description of Rhamnocercus dominguesi n. sp. and the redescription of Rhamnocercoides menticirrhi Luque and Iannacone, 1991
- Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions
- The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1
- Molecular characterization and functional analysis of a glutathione peroxidase gene from Aphelenchoides besseyi (Nematoda: Aphelenchoididae)
- Durettenema guangdongense gen. et sp. nov. (Nematoda: Molineoidea) from Hipposideros larvatus (Horsfield) (Chiroptera: Rhinolophidae) with discussion of the taxonomic status of Macielia rhinolophi Yin, 1980
- In vitro effects of purine and pyrimidine analogues on Leishmania donovani and Leishmania infantum promastigotes and intracellular amastigotes
- Population structure of the monogenean, Paramazocraes thrissocles (Mazocraeidae) on the gills of clupeoid fish, Thryssa mystax of Visakhapatnam coast, Bay of Bengal
- Redescription of Eutarsopolipus elongatus Regenfuss, 1968 (Acari: Podapolipidae) parasitising carabid beetles, with first description of the male
- Seroprevalence and risk factors of Neospora spp. and Toxoplasma gondii infections among horses and donkeys in Nigeria, West Africa
- Spinicauda sumatrana sp. nov. (Nematoda: Heterakidae) from Ludeking’s Crested Dragon, Lophocalotes ludekingi (Agamidae), from the Bukit Barisan Range of Sumatra
- The systematic position and structure of the genus Leyogonimus Ginetsinskaya, 1948 (Platyhelminthes: Digenea) with comments on the taxonomy of the superfamily Microphalloidea Ward, 1901
- Leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches
- Intestinal coccidian parasites as an underestimated cause of travellers’ diarrhoea in Polish immunocompetent patients
- Hymenolepididae (Cestoda, Cyclophyllidea) of ciconiiform and pelecaniform birds: the genera Oschmarinolepis Spassky and Spasskaja, 1954 and Paraoschmarinolepis gen. n.
- Redescription of two species of Thaparocleidus (Monogenea: Dactylogyridae), with the description of T. armillatus sp. n. from Wallago attu and a phylogenetic analysis based on 18S rDNA sequences
- In vitro and in vivo studies of nematophagous fungi Arthrobotrys musiformis and Arthrobotrys robusta against the larvae of the trichostrongylides
- Three monogeneans parasitic on marine sciaenid fish from Peru including description of Cynoscionicola veranoi n. sp. (Microcotylidae), and redescription of C. americanus Tantaleán, Martínez and Escalante, 1987 and Hargicotyle sciaenae Oliva and Luque, 1989 (Diclidophoridae)
- New and previously described dactylogyrid species (Monogenoidea: Polyonchoinea) and a gastrocotylinean pre-adult (Heteronchoinea) from pomacentrid and caesionid (Perciformes) fishes from Lizard Island, Great Barrier Reef, Australia
- Erratum to: 2017, 62(1) should be 2017, 62(2)