Startseite The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1
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The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1

  • Hayriye Kirkoyun Uysal , Gokhan Tolga Adas , Kevser Atalik , Semih Altiparmak , Ozer Akgul , Suat Saribas , Mert Gurcan , Pelin Yuksel , Taner Yildirmak , Bekir Kocazeybek EMAIL logo , Tevhide Ziver und Yasar Ali Oner
Veröffentlicht/Copyright: 5. Juli 2017
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Acta Parasitologica
Aus der Zeitschrift Acta Parasitologica Band 62 Heft 3

Abstract

Opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (AIDS). We investigated the existence of opportunistic protozoans that significantly affect the quality of life in HIV-1 infected patients using conventional and molecular methods. The study group comprised 115 HIV-1 positive patients. In the identification of Cyclospora cayetanensis and Cryptosporidium, the formol-ether precipitation method was used and smears were evaluated in optical microscope by staining modified Ziehl–Neelsen (ZN). The primers and probes used for PCR were Heat shock protein 70 for C. cayetanensis and the oocysts wall protein for Cryptosporidium spp.. Cyclospora and Cryptosporidium spp. oocysts were detected in one and two patients, respectively, by staining, whereas we detected C. cayetanensis in three patients out of 115 (2.6%) by PCR, and Cryptosporidium spp. in a further three patients (2.6%). C. cayetensis was detected in patients with CD4 counts of 64 cells/μm, 182 cells/μm and 287 cells/μm, respectively. Cryptosporidium spp. was detected in patients with CD4 counts of 176 cells/μm, 241 cells/μm and 669 cells/μm. As conclusion, PCR method is faster and more sensitive than microscopic methods and to screen intestinal pathogens routinely in patients infected with HIV should not be neglected in developing countries like Turkey.

Keywords: Cyclospora cayetanensis; Cryptosporidium spp; HIV-1

  1. Conflict of interests None declared.

Ethics

This study was approved by the Non-Invasive Clinical Research Ethics Committee of Istanbul Medical Faculty (Number: 2012/54-911/, Date: 26 January 2012). All patients provided their informed consent to participate in the study.

Acknowledgements

This study was supported by the Research Fund of Istanbul University, Project No: 22451. English language edited by American Manuscript Editors.

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Received: 2016-7-17
Revised: 2017-2-23
Accepted: 2017-4-12
Published Online: 2017-7-5
Published in Print: 2017-9-26

© 2017 W. Stefański Institute of Parasitology, PAS

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  4. Molecular phylogeny of the Haplosplanchnata Olson, Cribb, Tkach, Bray and Littlewood, 2003, with a description of Schikhobalotrema huffmani n. sp.
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  16. Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions
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  46. Three monogeneans parasitic on marine sciaenid fish from Peru including description of Cynoscionicola veranoi n. sp. (Microcotylidae), and redescription of C. americanus Tantaleán, Martínez and Escalante, 1987 and Hargicotyle sciaenae Oliva and Luque, 1989 (Diclidophoridae)
  48. New and previously described dactylogyrid species (Monogenoidea: Polyonchoinea) and a gastrocotylinean pre-adult (Heteronchoinea) from pomacentrid and caesionid (Perciformes) fishes from Lizard Island, Great Barrier Reef, Australia
  50. Erratum to: 2017, 62(1) should be 2017, 62(2)
https://doi.org/10.1515/ap-2017-0067
Schlagwörter für diesen Artikel
Cyclospora cayetanensis; Cryptosporidium spp; HIV-1

Artikel in diesem Heft

  1. Review
  2. Snail species diversity impacts the infection patterns of Echinostoma spp.: Examples from field collected data
  4. Molecular phylogeny of the Haplosplanchnata Olson, Cribb, Tkach, Bray and Littlewood, 2003, with a description of Schikhobalotrema huffmani n. sp.
  6. Structuraland antigenic analysis of a new Rhoptry Pseudokinase Gene (ROP54) in Toxoplasma gondii
  8. Ultrastructure of the spermatozoon of Macvicaria obovata (Digenea: Opecoelidae), a parasite of Sparus aurata(Pisces: Teleostei) from the Gulf of Gabès, Mediterranean Sea
  10. Epidemiology of Toxoplasma gondii infection in native Tibetans in Tibet, China
  12. A PCR survey of vector-borne pathogens in different dog populations from Turkey
  14. Diplectanids (Monogenea) parasitic on sciaenid fish from Peru with the proposal of Pseudorhamnocercoides n. gen., the description of Rhamnocercus dominguesi n. sp. and the redescription of Rhamnocercoides menticirrhi Luque and Iannacone, 1991
  16. Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions
  18. The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1
  20. Molecular characterization and functional analysis of a glutathione peroxidase gene from Aphelenchoides besseyi (Nematoda: Aphelenchoididae)
  22. Durettenema guangdongense gen. et sp. nov. (Nematoda: Molineoidea) from Hipposideros larvatus (Horsfield) (Chiroptera: Rhinolophidae) with discussion of the taxonomic status of Macielia rhinolophi Yin, 1980
  24. In vitro effects of purine and pyrimidine analogues on Leishmania donovani and Leishmania infantum promastigotes and intracellular amastigotes
  26. Population structure of the monogenean, Paramazocraes thrissocles (Mazocraeidae) on the gills of clupeoid fish, Thryssa mystax of Visakhapatnam coast, Bay of Bengal
  28. Redescription of Eutarsopolipus elongatus Regenfuss, 1968 (Acari: Podapolipidae) parasitising carabid beetles, with first description of the male
  30. Seroprevalence and risk factors of Neospora spp. and Toxoplasma gondii infections among horses and donkeys in Nigeria, West Africa
  32. Spinicauda sumatrana sp. nov. (Nematoda: Heterakidae) from Ludeking’s Crested Dragon, Lophocalotes ludekingi (Agamidae), from the Bukit Barisan Range of Sumatra
  34. The systematic position and structure of the genus Leyogonimus Ginetsinskaya, 1948 (Platyhelminthes: Digenea) with comments on the taxonomy of the superfamily Microphalloidea Ward, 1901
  36. Leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches
  38. Intestinal coccidian parasites as an underestimated cause of travellers’ diarrhoea in Polish immunocompetent patients
  40. Hymenolepididae (Cestoda, Cyclophyllidea) of ciconiiform and pelecaniform birds: the genera Oschmarinolepis Spassky and Spasskaja, 1954 and Paraoschmarinolepis gen. n.
  42. Redescription of two species of Thaparocleidus (Monogenea: Dactylogyridae), with the description of T. armillatus sp. n. from Wallago attu and a phylogenetic analysis based on 18S rDNA sequences
  44. In vitro and in vivo studies of nematophagous fungi Arthrobotrys musiformis and Arthrobotrys robusta against the larvae of the trichostrongylides
  46. Three monogeneans parasitic on marine sciaenid fish from Peru including description of Cynoscionicola veranoi n. sp. (Microcotylidae), and redescription of C. americanus Tantaleán, Martínez and Escalante, 1987 and Hargicotyle sciaenae Oliva and Luque, 1989 (Diclidophoridae)
  48. New and previously described dactylogyrid species (Monogenoidea: Polyonchoinea) and a gastrocotylinean pre-adult (Heteronchoinea) from pomacentrid and caesionid (Perciformes) fishes from Lizard Island, Great Barrier Reef, Australia
  50. Erratum to: 2017, 62(1) should be 2017, 62(2)
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