Startseite Medizin Disruption of egg production by triclabendazole-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (Mirazid)
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Disruption of egg production by triclabendazole-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (Mirazid)

  • Mohamed M.O. Abdelaal , Gerard P. Brennan , Robert E.B. Hanna , Ahmed Abdel-Aziz und Ian Fairweather EMAIL logo
Veröffentlicht/Copyright: 18. April 2017
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Acta Parasitologica
Aus der Zeitschrift Acta Parasitologica Band 62 Heft 2

Abstract

An in vitro study has been carried out to monitor changes to the female reproductive system in adult triclabendazole (TCBZ)-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (“Mirazid”). Flukes were immersed for 6 h and 24 h in myrrh extract at a concentration of 200 µg/ml, then processed for histological and transmission electron microscope (TEM) examination of the uterus, Mehlis’ gland, ovary and vitellaria. Egg production had become abnormal at 6 h post-treatment (pt), with the uterine lumen being filled with free vitelline cells and masses of shell protein material; few eggs were present. At 24 h pt, no eggs were present. Distinct changes to the ovary and Mehlis’ gland were only observed after 24 h incubation in Mirazid. The ovary contained numbers of apoptotic oogonia and oocytes. In the Mehlis’ gland, the S1 cells were disorganised and the processes from them were vacuolated, although the disruption was not significant. More severe changes were observed in the vitelline cells and follicles. After 6 h incubation in Mirazid, although the gross organisation of the vitelline follicles appeared to be normal, nuclear changes indicative of the early stages of apoptosis were observed in the stem cells and shell protein production by the mature cells had decreased. At 24 h pt, a distinct shift in cell population was evident, with the follicles containing mainly mature cells and spaces were present between the cells. The shell globule clusters in the mature cells were disorganised. In more severely-affected follicles, cells were seen to be breaking down, with karyolytic nuclei and disintegrating cytoplasm. Overall, the results have shown that exposure to Mirazid treatment had a severe impact on egg production by TCBZ-resistant flukes, an effect that was mediated by disruption of the vitelline cells and of the mechanism co-ordinating egg formation in the ootype.

Keywords: Fasciola hepatica; liver fluke; Mirazid; in vitro; transmission electron microscopy; histology; vitellaria; ovary; uterus; Mehlis’ gland; egg production

Acknowledgments

Mohamed Abdelaal was a Visiting Research Associate at The Queen’s University of Belfast. He was in receipt of an Egyptian Government Scholarship under the Channel Scheme.

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Received: 2016-7-15
Revised: 2016-11-13
Accepted: 2017-1-12
Published Online: 2017-4-18
Published in Print: 2017-6-1

© 2017 W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2017-0041
Schlagwörter für diesen Artikel
Fasciola hepatica; liver fluke; Mirazid; in vitro; transmission electron microscopy; histology; vitellaria; ovary; uterus; Mehlis’ gland; egg production

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  12. Amoebicidal activity of α-bisabolol, the main sesquiterpene in chamomile (Matricaria recutita L.) essential oil against the trophozoite stage of Acanthamoeba castellani Neff
  14. A new species of Oochoristica (Cyclophyllidea: Linstowiidae) from non-native Mediterranean geckos, Hemidactylus turcicus (Sauria: Gekkonidae), from Texas, USA
  16. The ruby-crowned tanager Tachyphonus coronatus Vieillot, 1822 (Passeriformes: Thraupidae) as a new host for Isospora ramphoceli Berto, Flausino, Luz, Ferreira, Lopes, 2010 in Brazil
  18. Ecological implications of floods on the parasite communities of two freshwater catfishes in a Neotropical floodplain
  20. Morphological re-description of Electrotaenia malapteruri (Cestoda: Proteocephalidae) and Dujardinnascarismalapteruri (Nematoda: Heterocheilidae) infecting the Electric catfish Malapterurus electricus and heavy metal accumulation in host and parasites in relation to water and sediment analysis in Lake Manzala, North Delta, Egypt
  22. Disruption of egg production by triclabendazole-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (Mirazid)
  24. How does host ecology influence sampling effort in parasite diversity estimates? A case study using Neotropical freshwater fishes
  26. cDNA library construction of two human Demodexspecies
  28. Helminth (Cestoda, Nematoda) and coccidian (Apicomplexa: Eimeriidae) parasites of the eastern small-footed myotis, Myotis leibii (Chiroptera: Vespertilionidae) from Arkansas, with a description of a new species of Eimeria
  30. Molecular identification of Thelandros scleratus and Thelastoma icemi (Nematoda: Oxyruida) using mitochondrial cox 1 sequences
  32. First report on molecular evidence of Tylodelphys cerebralis (Diplostomulum cerebralis) Chakrabarti, 1968 (Digenea: Diplostomidae) from snakehead fish Channa punctata
  34. The role of domestic dogs in the transmission of zoonotic helminthes in a rural area of Mekong river basin
  36. The discovery of acanthocephalans parasitizing chaetognaths
  38. Redescription of Philometra margolisiMoravec, Vidal-Martínez et Aguirre-Macedo, 1995 (Nematoda: Philometridae), a gonad-infecting parasite of the red grouper Epinephelus morio (Serranidae) in the Gulf of Mexico
  40. Nematode parasites of the Chilean Flamingo, Phoenicopterus chilensis (Phoenicopteridae) from Central Argentina, with a description of a new species of Tetrameres (Tetrameridae)
  42. Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
  44. Morphological description of Cosmocerca sp. (Nematoda: Cosmocerdidae) from the Mascarene grass frog Ptychadena cf. mascareniensis (Amphibia: Ptychadenidae). A light and scanning electron microscopic studies
  46. Detection of Echinococcus multilocularis DNA in fruit, vegetable, and mushroom samples collected in the non-endemic territory of the Pomerania province and comparison of the results with data from rural areas of the neighbouring highly endemic Warmia-Masuria province, Poland
  48. Toxoplasma gondii in backyard pigs: seroepidemiology and mouse bioassay
  50. Infection of the lesser spotted dogfish with Proleptus obtusus Dujardin, 1845 (Nematoda: Spirurida) reflects ontogenetic feeding behaviour and seasonal differences in prey availability
  52. A new species of the quill mite genus ChenophilaKethley, 1970 (Acariformes: Syringophilidae) from the Marbled Teal Marmaronetta angustirostris (Menetries) (Anseriformes: Anatidae) in Turkey
  54. Neuropathological lesions of clinical and sub clinical Coenurosis (Coenurus cerebralis) in organized goat farms in India
  55. Case Report
  56. Fatal aelurostrongylosis in a kitten in Serbia
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Heruntergeladen am 24.12.2025 von https://www.degruyterbrill.com/document/doi/10.1515/ap-2017-0041/pdf
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