Abstract
The impact of sugar moieties of Toxocara canis glycoprotein antigens on their recognition by infected mouse antibodies was investigated in this study. Native TES and recombinant Toxocara mucins generated in Pichia pastoris yeast as well as their deglycosylated forms were used in ELISA. TES and recombinant mucins were equally recognized by T. canis infected mouse IgG1 antibodies. IgM immunoglobulins predominantly recognized TES antigens. Among mucins recognition of Tc-MUC-4 was the most significant. Deglycosylation of antigens resulted in significant loss of IgM and IgG1 reactivity to TES, mucins, Tc-MUC-3 and Tc-MUC-4. The presence of sugar moieties had no influence on IgE binding to native or recombinant T. canis antigens. Our results suggest that glycans are involved in epitope formation what should be taken into consideration in production of recombinant helminth antigens for diagnostic purposes.
References
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© 2016
Articles in the same Issue
- Toxocarosis of the organ of sight – the complex pathological and diagnostic problem
- Trichomonas vaginalis: An Updated Overview Towards Diagnostic Improvement
- New species and records of mites of the superfamily Sarcoptoidea (Acariformes: Psoroptidia) from mammals in Brazil
- The morphology of free-living stages and immature parasites of Rhabdias paraensis (Nematoda: Rhabdiasidae), a parasite of Rhinella marina (Anura: Bufonidae) in Brazil
- Effects of altered water quality and trace elements on the infection variables of Paradiplozoon ichthyoxanthon (Monogenea: Diplozoidae) from two sites in the Vaal River system, South Africa
- Herd-level seroprevalence of Neospora caninum infection in dairy cattle in central and northeastern Poland
- Ultrastructure of Ascaridia galli (Schrank, 1788) (Nematoda: Ascaridida) from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae)
- Prevalence and risk factors associated to Eimeria spp. infection in unweaned alpacas (Vicugna pacos) from Southern Peru
- Description of a new species of Chabaudus Inglis and Ogden, 1965 (Nematoda: Seuratoidea) from the frog Euphlyctis cyanophlyctis from Dehrandun, Uttarakhand, India
- Fertilization in the cestode Echinococcus multilocularis (Cyclophyllidea, Taeniidae)
- Expression analysis of viscerotropic leishmaniasis gene in Leishmania species by real-time RT-PCR
- Fourier transform infrared spectroscopy as a tool for identification of crude microbial extracts with anti-malarial potential
- A 43 kDa recombinant plasmepsin elicits immune response in mice against Plasmodium berghei malaria
- Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography
- In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis
- Seasonal pattern in parasite infracommunities of Hoplerythrinus unitaeniatus and Hoplias malabaricus (Actinopterygii: Erythrinidae) from the Brazilian Amazon
- The first detection of the tick-borne encephalitis virus (TBEV) RNA in Dermacentor reticulatus ticks collected from the lowland European bison (Bison bonasus bonasus L.)
- Assessing the influence of geographic distance in parasite communities of an exotic lizard
- Diagnostic value of semi-purified antigens of hydatid cyst fluid in human cystic echinococcosis
- Occurrence and morphogenetic characteristics of Gyrodactylus (Monogenea: Gyrodactylidae) from a rainbow trout farm (Lake Ladoga, Russia)
- Dactylogyrids (Monogenea) parasitic on cichlids from northern Brazil, with description of two new species of Sciadicleithrum and new host and geographical records
- Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey
- First report of Cystoisospora belli parasitemia in a patient with acquired immunodeficiency syndrome
- Cystic echinococcosis in Southern Israel
- Larval cestodes infecting the deep-water fish, Cataetyx laticeps (Pisces: Bythitidae) from Madeira Archipelago, Atlantic Ocean
- Toxocara canis glycans influence antigen recognition by mouse IgG1 and IgM antibodies
- Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences
- The transstadial persistence of tick-borne encephalitis virus in Dermacentor reticulatus ticks in natural conditions
Articles in the same Issue
- Toxocarosis of the organ of sight – the complex pathological and diagnostic problem
- Trichomonas vaginalis: An Updated Overview Towards Diagnostic Improvement
- New species and records of mites of the superfamily Sarcoptoidea (Acariformes: Psoroptidia) from mammals in Brazil
- The morphology of free-living stages and immature parasites of Rhabdias paraensis (Nematoda: Rhabdiasidae), a parasite of Rhinella marina (Anura: Bufonidae) in Brazil
- Effects of altered water quality and trace elements on the infection variables of Paradiplozoon ichthyoxanthon (Monogenea: Diplozoidae) from two sites in the Vaal River system, South Africa
- Herd-level seroprevalence of Neospora caninum infection in dairy cattle in central and northeastern Poland
- Ultrastructure of Ascaridia galli (Schrank, 1788) (Nematoda: Ascaridida) from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae)
- Prevalence and risk factors associated to Eimeria spp. infection in unweaned alpacas (Vicugna pacos) from Southern Peru
- Description of a new species of Chabaudus Inglis and Ogden, 1965 (Nematoda: Seuratoidea) from the frog Euphlyctis cyanophlyctis from Dehrandun, Uttarakhand, India
- Fertilization in the cestode Echinococcus multilocularis (Cyclophyllidea, Taeniidae)
- Expression analysis of viscerotropic leishmaniasis gene in Leishmania species by real-time RT-PCR
- Fourier transform infrared spectroscopy as a tool for identification of crude microbial extracts with anti-malarial potential
- A 43 kDa recombinant plasmepsin elicits immune response in mice against Plasmodium berghei malaria
- Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography
- In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis
- Seasonal pattern in parasite infracommunities of Hoplerythrinus unitaeniatus and Hoplias malabaricus (Actinopterygii: Erythrinidae) from the Brazilian Amazon
- The first detection of the tick-borne encephalitis virus (TBEV) RNA in Dermacentor reticulatus ticks collected from the lowland European bison (Bison bonasus bonasus L.)
- Assessing the influence of geographic distance in parasite communities of an exotic lizard
- Diagnostic value of semi-purified antigens of hydatid cyst fluid in human cystic echinococcosis
- Occurrence and morphogenetic characteristics of Gyrodactylus (Monogenea: Gyrodactylidae) from a rainbow trout farm (Lake Ladoga, Russia)
- Dactylogyrids (Monogenea) parasitic on cichlids from northern Brazil, with description of two new species of Sciadicleithrum and new host and geographical records
- Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey
- First report of Cystoisospora belli parasitemia in a patient with acquired immunodeficiency syndrome
- Cystic echinococcosis in Southern Israel
- Larval cestodes infecting the deep-water fish, Cataetyx laticeps (Pisces: Bythitidae) from Madeira Archipelago, Atlantic Ocean
- Toxocara canis glycans influence antigen recognition by mouse IgG1 and IgM antibodies
- Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences
- The transstadial persistence of tick-borne encephalitis virus in Dermacentor reticulatus ticks in natural conditions