Herd-level seroprevalence of Neospora caninum infection in dairy cattle in central and northeastern Poland
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Sławomir J. Kowalczyk
Abstract
A serosurvey was carried out to estimate the herd-level seroprevalence of Neospora caninum infection in cattle in central and northeastern Poland. Ninety seven dairy cattle herds from 2 provinces of Poland (Podlaskie, 47 herds and Łodzkie, 50 herds) were randomly enrolled in the study using two-stage cluster method. A simple random selection was applied within each herd to select a sample of adult cows (≥18 month-old). A total number of 734 cows were enrolled in the study. The animals were screened with a commercial competitive ELISA (Bio-X Diagnostics, Belgium). To calculate true herd-level seroprevalence test sensitivity and specificity were adjusted from an individual- to a herd-level using FreeCalc method. The true overall herd-level seroprevalence of N. caninum infection was 56.7% (95% CI: 47.5%, 65.9%). The true herd-level seroprevalence in Podlaskie was 63.3% (95% CI: 43.0%, 83.6%) and 50.5% (95% CI: 32.8%, 68.2%) in Łodzkie province and these figures did not differ significantly between the two provinces (chi2 test p = 0.238). One hundred forty three of 734 cows (19.5%) were seropositive which gave the true overall individual-level seroprevalence of 20.1% (95% CI: 17.4%, 23.2%). Percentage of seropositive cows in each herd varied from 6% to 80%. This study is the first epidemiological investigation of herd-level seroprevalence of N. caninum infection in Polish dairy cattle population. In conclusion, the result of the study confirmed previous data that N. caninum infection is widespread in the Polish cattle population and thus should be considered as a potential cause of spontaneous abortions.
References
Altman D.G., Machin D., Bryant T.N., Gardner M.J. (Eds.). 2000. Statistics with confidence. BMJ Books. pp. 45-57Search in Google Scholar
Bartels C.J., Arnaiz-Seco J.I., Ruiz-Santa-Quitera A., Björkman C., Frössling J., von Blumröder D., Conraths F.J., Schares G., van Maanen C., Wouda W., Ortega-Mora L.M. 2006. Supranational comparison of Neospora caninum seroprevalences in cattle in Germany, The Netherlands, Spain and Sweden. Veterinary Parasitology, 137, 17-27. DOI: 10.1016/j.vetpar. 2005.12.016Search in Google Scholar
Bártová E., Sedlak K., Budíková M. 2015. A study of Neospora caninum and Toxoplasma gondii antibody seroprevalence in healthy cattle in the Czech Republic. Annals of Agricultural and Environmental Medicine, 22, 32-3410.5604/12321966.1141365Search in Google Scholar
Bień J., Moskwa B., Bogdaszewski M., Cabaj W. 2012. Detection of specific antibodies anti-Neospora caninum in the fallow deer (Dama dama). Research in Veterinary Science, 92, 96-98. DOI: 10.1016/j.rvsc.2010.10.01410.1016/j.rvsc.2010.10.014Search in Google Scholar
Bień J., Moskwa B., Cabaj W. 2010. In vitro isolation and identification of the first Neospora caninum isolate from European bison (Bison bonasus bonasus L.). Veterinary Parasitology, 173, 200-205. DOI: 10.1016/j.vetpar.2010.06.03810.1016/j.vetpar.2010.06.038Search in Google Scholar
Cabaj W., Choromański L., Rodgers S., Moskwa B., Malczewski A. 2000. Neospora caninum infections in dairy cows with abortion in Poland. Acta Parasitologica, 45, 113-114Search in Google Scholar
Cabaj W., Moskwa B., Pastusiak K., Gill J. 2005. Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland. Veterinary Parasitology, 128, 163-16810.1016/j.vetpar.2004.09.033Search in Google Scholar
Cameron A.R., Baldock F.C. 1998. A new probability formula for surveys to substantiate freedom from disease. Preventive Veterinary Medicine, 34, 1-1710.1016/S0167-5877(97)00081-0Search in Google Scholar
Čobádiová A., Víchová B., Majláthová V., Reiterová K. 2013. First molecular detection of Neospora caninum in European brown bear (Ursus arctos). Veterinary Parasitology, 197, 346-349. DOI: 10.1016/j.vetpar.2013.05.00510.1016/j.vetpar.2013.05.005Search in Google Scholar PubMed
Czopowicz M., Kaba J., Szaluś-Jordanow O., Nowicki M., Witkowski L., Frymus T. 2011. Seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Poland. Veterinary Parasitology, 178, 339-341. DOI: 10.1016/j.vetpar.2011.01.03910.1016/j.vetpar.2011.01.039Search in Google Scholar PubMed
Dubey J.P. 2003. Review of Neospora caninum and neosporosis in animals. Korean Journal of Parasitology, 41, 1-1610.3347/kjp.2003.41.1.1Search in Google Scholar PubMed PubMed Central
Goodswen S.J., Kennedy P.J., Ellis J.T. 2013. A review of the infection, genetics, and evolution of Neospora caninum: from the past to the present. Infection, Genetics and Evolution, 13, 133-150. DOI: 10.1016/j.meegid.2012.08.01210.1016/j.meegid.2012.08.012Search in Google Scholar PubMed
Goździk K., Jakubek E.B., Björkman C., Bień J., Moskwa B., Cabaj W. 2010. Seroprevalence of Neospora caninum in free living and farmed red deer (Cervus elaphus) in Poland. Polish Journal of Veterinary Sciences, 13, 117-120Search in Google Scholar
Goździk K., Grono K. 2009. Neosporosis as an abortion cause in cattle. Magazyn Weterynaryjny, 18, 230-234 Search in Google Scholar
Goździk K., Grono K. 2010. Neospora caninum - a dangerous parasite responsible for abortion in dairy cattle. Magazyn Weterynaryjny. Choroby bydła - Monografia, 1030-1033Search in Google Scholar
Goździk K., Wrzesień R., Wielgosz-Ostolska A., Bień J., Kozak- Ljunggren M., Cabaj W. 2011. Prevalence of antibodies against Neospora caninum in dogs from urban areas in Central Poland. Parasitology Research, 108, 991-996. DOI: 10.1007/s00436-010-2143-010.1007/s00436-010-2143-0Search in Google Scholar PubMed PubMed Central
Hornok S., Edelhofer R., Hajtos I. 2006. Seroprevalence of neosporosis in beef and dairy cattle breeds in Northeast Hungary. Acta Veterinaria Hungarica, 54, 485-49110.1556/AVet.54.2006.4.6Search in Google Scholar PubMed
Machačová T., Bártová E., Di Loria A., Sedlák K., Guccione J., Fulgione D., Veneziano V. 2013. Seroprevalence and risk factors of Neospora spp. in donkeys from Southern Italy. Veterinary Parasitology, 198, 201-204. DOI: 10.1016/j.vetpar.2013.08.01310.1016/j.vetpar.2013.08.013Search in Google Scholar PubMed
Moskwa B., Goździk K., Bień J., Kornacka A., Cybulska A., Reiterova K., Cabaj W. 2014. Detection of antibodies to Neospora caninum in moose (Alces alces): the first report in Europe. Folia Parasitologica, 61, 34-3610.14411/fp.2014.014Search in Google Scholar
Płoneczka K., Mazurkiewicz M. 2008. Seroprevalence of Neospora caninum in dogs in south-western Poland. Veterinary Parasitology, 153, 168-171. DOI: 10.1016/j.vetpar.2008.01.00710.1016/j.vetpar.2008.01.007Search in Google Scholar PubMed
Reichel M.P., Alejandra Ayanegui-Alcérreca M., Gondim L.F., Ellis J.T. 2013. What is the global economic impact of Neospora caninum in cattle - the billion dollar question. International Journal of Parasitology, 43, 133-142. DOI: 10.1016/j.ijpara. 2012.10.022Search in Google Scholar
Reiterová K., Spilovská S., Antolová D., Dubinský P. 2009. Neospora caninum, potential cause of abortions in dairy cows: the current serological follow-up in Slovakia. Veterinary Parasitology, 159, 1-6. DOI: 10.1016/j.vetpar.2008.10.00810.1016/j.vetpar.2008.10.008Search in Google Scholar PubMed
Sergeant E.S.G. 2015. Epitools epidemiological calculators. AusVet Animal Health Services and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease. Available at: http://epitools.ausvet.com.au Search in Google Scholar
Thrusfield M. 2005. Veterinary epidemiology. 3rd ed., Blackwell Publishing Ltd. p. 230, 238-240, 245Search in Google Scholar
Tomczuk K., Radzikowski A.B., Studzińska M.B., Witkowska A., Puchalski A. 2007. Antibodies to Neospora caninum in sera of cows from medium and small farms near Lublin. Annales Universitatis Mariae Curie-Skłodowska Lublin-Polonia, LXII, 50-53Search in Google Scholar
Wapenaar W., Barkema H.W., Vanleeuwen J.A., McClure J.T., O’Handley R.M., Kwok O.C., Thulliez P., Dubey J.P., Jenkins M.C. 2007. Comparison of serological methods for the diagnosis of Neospora caninum infection in cattle. Veterinary Parasitology, 143, 166-173. DOI: 10.1016/j.vetpar.2006.08.007 10.1016/j.vetpar.2006.08.007Search in Google Scholar PubMed
© 2016
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- Toxocarosis of the organ of sight – the complex pathological and diagnostic problem
- Trichomonas vaginalis: An Updated Overview Towards Diagnostic Improvement
- New species and records of mites of the superfamily Sarcoptoidea (Acariformes: Psoroptidia) from mammals in Brazil
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Articles in the same Issue
- Toxocarosis of the organ of sight – the complex pathological and diagnostic problem
- Trichomonas vaginalis: An Updated Overview Towards Diagnostic Improvement
- New species and records of mites of the superfamily Sarcoptoidea (Acariformes: Psoroptidia) from mammals in Brazil
- The morphology of free-living stages and immature parasites of Rhabdias paraensis (Nematoda: Rhabdiasidae), a parasite of Rhinella marina (Anura: Bufonidae) in Brazil
- Effects of altered water quality and trace elements on the infection variables of Paradiplozoon ichthyoxanthon (Monogenea: Diplozoidae) from two sites in the Vaal River system, South Africa
- Herd-level seroprevalence of Neospora caninum infection in dairy cattle in central and northeastern Poland
- Ultrastructure of Ascaridia galli (Schrank, 1788) (Nematoda: Ascaridida) from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae)
- Prevalence and risk factors associated to Eimeria spp. infection in unweaned alpacas (Vicugna pacos) from Southern Peru
- Description of a new species of Chabaudus Inglis and Ogden, 1965 (Nematoda: Seuratoidea) from the frog Euphlyctis cyanophlyctis from Dehrandun, Uttarakhand, India
- Fertilization in the cestode Echinococcus multilocularis (Cyclophyllidea, Taeniidae)
- Expression analysis of viscerotropic leishmaniasis gene in Leishmania species by real-time RT-PCR
- Fourier transform infrared spectroscopy as a tool for identification of crude microbial extracts with anti-malarial potential
- A 43 kDa recombinant plasmepsin elicits immune response in mice against Plasmodium berghei malaria
- Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography
- In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis
- Seasonal pattern in parasite infracommunities of Hoplerythrinus unitaeniatus and Hoplias malabaricus (Actinopterygii: Erythrinidae) from the Brazilian Amazon
- The first detection of the tick-borne encephalitis virus (TBEV) RNA in Dermacentor reticulatus ticks collected from the lowland European bison (Bison bonasus bonasus L.)
- Assessing the influence of geographic distance in parasite communities of an exotic lizard
- Diagnostic value of semi-purified antigens of hydatid cyst fluid in human cystic echinococcosis
- Occurrence and morphogenetic characteristics of Gyrodactylus (Monogenea: Gyrodactylidae) from a rainbow trout farm (Lake Ladoga, Russia)
- Dactylogyrids (Monogenea) parasitic on cichlids from northern Brazil, with description of two new species of Sciadicleithrum and new host and geographical records
- Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey
- First report of Cystoisospora belli parasitemia in a patient with acquired immunodeficiency syndrome
- Cystic echinococcosis in Southern Israel
- Larval cestodes infecting the deep-water fish, Cataetyx laticeps (Pisces: Bythitidae) from Madeira Archipelago, Atlantic Ocean
- Toxocara canis glycans influence antigen recognition by mouse IgG1 and IgM antibodies
- Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences
- The transstadial persistence of tick-borne encephalitis virus in Dermacentor reticulatus ticks in natural conditions
