Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil
-
Luciamáre P.A. Martins
Abstract
An isolate of Trypanosoma cruzi obtained from P. megistus captured in the peridomicile area of a home in Santo Antonio do Jardim city in the State of Sao Paulo, denominated T. cruzi Mogi, was characterized biologically and molecularly. The RFLP analysis of the D7 divergent domain in the 24Sα rDNA and of the mini-exon positioned the T. cruzi isolate within the TcI group. Phylogenetic analysis performed with the trypanosomatid barcode confirmed that the isolate belongs to the TcI group, with high homology to the 3014 c1 T.cruzi strain. The biological characterization of the isolate in rats showed a prepatent period of about 8 days, low parasitemia and tropism for cardiac, skeletal and colonic muscles. In Swiss mice the T. cruzi Mogi isolate showed a prepatent period of about 22 days, intermittent parasitemia in some animals, and tropism for cardiac and colonic muscles. Despite the inherent difficulty of identifying correlations amongst the molecular and biological characteristics of different T. cruzi groups, the tropism for colonic muscle demonstrated by T. cruzi Mogi represented a peculiarity of this isolate within the TcI group.
References
Almeida E.A., Silva E.L., Guariento M.E., Souza M.L., Aoki F.H., Pedro R.P. 2009. Evolucao fatal da co-infeccao doenca de Chagas/Aids: dificuldades diagnosticas entre a reagudizacao da miocardite e a miocardiopatia chagasica cronica. Revista da Sociedade Brasileira de Medicina Tropical, 42, 199-202Search in Google Scholar
Andrade S.G. 1974. Caracterizacao de cepas do Trypanosoma cruzi isoladas no Reconcavo Baiano (contribuicao ao estudo geral da doenca de Chagas em nosso meio). Revista de Patologia Tropical, 3, 65-121Search in Google Scholar
Araujo C.A.C.,Waniek P.J., Jansen A.M. 2009. An overview of Chagas disease and the role of Triatomines on its distribution in Brazil. Vector-Borne and Zoonotic diseases, 9, 227-234Search in Google Scholar
Ayala J.M.L. 2009. Una nueva especie de Panstrongylus Berg de Venezuela (Hemiptera: Reduviidae, Triatominae). Entomotropica, 24, 105-109Search in Google Scholar
Azambuja P., Ratcliffe N.A., Garcia E.S. 2005. Towards an understanding of the interactions of Trypanosoma cruzi and Trypanosoma rangeli within the reduviid insect host Rhodnius prolixus. Annals of the Brazilian Academy of Sciences, 77, 397-404Search in Google Scholar
Brener Z. 1962. Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi. Revista do Instituto de Medicina Tropical de São Paulo, 4, 389-396.Search in Google Scholar
Coura J.R. 2007. Origem, determinentes e morbidade da doenca de Chagas. Salus, 11, 62-66Search in Google Scholar
Coura J.R., Dias J.C.P. 2009. Epidemiology, control and surveillance of Chagas disease - 100 years after its discovery. Memórias do Instituto Oswaldo Cruz, 104, 31-40Search in Google Scholar
Coura J.R., Borges-Pereira J. 2012. Chagas disease. What is know and what should be improved: a systematic review. Revista da Sociedade Brasileira de Medicina Tropical, 45, 286-296Search in Google Scholar
Devera R., Fernandes O., Coura J.F. 2003. Should Trypanosoma cruzi be called “cruzi” complex? A review of the parasite diversity and the potential of selecting population after in vitro culturing and mice infection. Memorias do Instituto Oswaldo Cruz, 98, 1-12Search in Google Scholar
Dias J.A.P. 2006. Doencas de Chagas. Cadernos de Saúde Pública, 22, 2020-21Search in Google Scholar
Dias J.C.P. 2009. Elimination of Chagas disease transmission: perspectives. Memórias do Instituto Oswaldo Cruz, 104, 41-45Search in Google Scholar
Fernandes O., Santos S.S., Cupolillo E., Mendonca B., Derre R., Junqueira AC., Santos L.C., Sturm N.R., Naiff R.D., Barret T.V., Campbell D.A., Coura J.R. 2001.A miniexon multiplex polymerase chain reaction to distinguish the major groups of Trypanosoma cruzi and T. rangeli in the Brazilian Amazon. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95, 97-99Search in Google Scholar
Goncalves T.C.M., Teves-Neves S.C., Santos-Mallet J.R., Carbajalde- la-Fuente A.L., Lopes C.M. 2013. Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Memórias do Instituto Oswaldo Cruz, 108, 429-437Search in Google Scholar
Guhl F., Ramirez J.D. 2011. Trypanosoma cruzi I diversity: towards the need of genetic subdivision? Acta Tropica, 119, 1-4Search in Google Scholar
Gurgel-Goncalves R., Galvao C., Costa J., Peterson T.A. 2012. Geografic distribuition of Chagas disease vectors in Brasil based on ecological niche modeling. Journal of Tropical Medicine, 2012, ID 705326, 15 pages, DOI:10.1155/2012/705326Search in Google Scholar
Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98NT. Nucleic Acids Symposium 41, 95-98Search in Google Scholar
Kumar K., Murphy J.D., Hisgen A.L. 2004. Method and apparatus for dynamically altering configurations of clustered computer systems. United States Patent US 6,769,008 B1, 27 jul Search in Google Scholar
Lima L., Silva F.M., Neves L., Azias M., Takata C.S., Campaner M., de Souza W., Hamilton P.B., Teixeira M.M. 2012. Evolutionary insights from bat trypanosomes: morphological, developmental and phylogenetic evidence of a new species Trypanosoma (Schizotrypanum erneyi sp. nov., in African bats closely related to Trypanosoma (Schizotrypanum) cruzi and allied species. Protist, 163, 856-872Search in Google Scholar
Lisboa C.V., Pinho A.P., Monteiro R.V., Jansen A.M. 2007. Trypanosoma cruzi (kinetoplastidae Trypanosomatidae): Biological heterogeneity in the isolates derived from wild hosts. Experimental Parasitology, 116, 150-155Search in Google Scholar
Litvoc J., Goldbaum M., Silva G.R. 1990. Determinantes do processo de infestacao domiciliar por Panstrongylus megistus: o papel da habitacao e do desmatamento. Revista do Instituto de Medicina Tropical de São Paulo, 32, 443-9Search in Google Scholar
Martins L.P.A., Castanho R.E.P., Rosa J.A., Silva L.C., Godoy C.A.P., Rosa R.M. 2003. Caracterizacao biologica, histopatologica e analise de acido nucleico de uma cepa Trypanosoma cruzi da regiao de Marilia, SP. Revista da Sociedade Brasileira de Medicina Tropical, 36, 35-39 Search in Google Scholar
Mendonca M.B., Nehme N.S., Santos S.S., Cupolillo E., Vargas N., Junqueira A., Naiff R.D., Barrett T.V., Coura J.R., Zingales B., Fernandes O. 2002. Two main clusters within Trypanosoma cruzi zymodeme 3 are defined by distinct regions of the ribosomal RNA cistron. Parasitology, 124, 177-184Search in Google Scholar
Miles M.A., Souza A.A.A., Povoa M.M. 1982. O ecotopo do Panstrongylus megistus (Hemiptera, Reduviidae) na floresta do Horto (Rio de Janeiro). Revista Brasileira de Biologia, 42, 31-5Search in Google Scholar
Monteiro W.M., Magalhaes L.K.C., Oliveira J.C., Guerra J.A.O., Silveira H., Ferreira L.C.L., Toledo M.J.O., Barbosa M.G.V. 2012. Biological behavior of Trypanosoma cruzi stocks obtained from the State of Amazonas, Western Brazilian Amazon, in mice. Revista da Sociedade Brasileira de Medicina Tropical, 45, 209-14Search in Google Scholar
Patterson J.S., Barbosa S.E., Feliciangeli M.D. 2009. On the genus Panstrongylus Berg 1879: evolution, ecology and epidemiological significance. Acta Tropica, 110, 187-99Search in Google Scholar
Ramirez J.D., Guhl F., Rendon L.M., Rosas F., Marin-Neto J.A., Morillo C.A. 2010. Chagas Cardiomyopathy Manifestations and Trypanosoma cruzi Genotypes Circulating in Chronic Chagasic Patients. PLOS Neglected Tropical Diseases, 4, e899. DOI: 10.1371/journal.pntd.0000899/ Search in Google Scholar
Reis D., Monteiro W.M., Bossolani G.D.P., Teston A.P.G., Gomes M.L., Araujo S.M., Barbosa M.G.V., Toledo M.J.O. 2012. Biological behavior in mice of Ttypanosoma cruzi isolates from Amazonas and Parana, Brazil. Experimental Parasitology, 130, 321-329Search in Google Scholar
Rodrigues V.L.C.C., Zingales B., Zacharias F., Silva M.N., Tonietti V.L.B., Silva R.A. 2003. Caracterizacao de uma cepa de Trypanosoma cruzi encontrada no municipio de Sao Paulo, Estado de Sao Paulo, Brasil. Revista da Socidade Brasileira de Medicina Tropical, 36, supl 2Search in Google Scholar
Rodrigues V.L.C.C., Casanova C., Silva R.A., Baitelo D., Tonietti V.L.B. 2009. Circulacao de Trypanosoma cruzi em animais proximos ao homem em localidades adjacemtes a encosta da Serra da Mantiqueira Paulista. Revista da Sociedade Brasileira de Medicina Tropical, 42, 465Search in Google Scholar
Rosa J.A., Rocha C.S., Gardim S., Pinto M.C., Mendonca V.J., Ferreira Filho J.C.R., Carvalho E.O.C., Camargo L.M.A., Oliveira J., Nascimento J.D., Cilense M., Almeida C.E. 2012. Description of Rhodnius montenegrensis n. sp. (Hemiptera: Reduviidae: Triatominae) from the state of Rondonia, Brazil. Zootaxa, 3478, 62-76Search in Google Scholar
Sanchez-Guillen M.C., Bernabe C., Tibayrenc M., Zavala-Castro J., Totolhua J.-L., Mendez-Lopez J., Gonzalez-Mejia M.-E., Torres- Rasgado E., Lopez-Colombo A., Perez-Fuentes R. 2006. Trypanosoma cruzi strains isolated from human, vector, and animal reservior in the same endemic region in Mexico and typed as T. cruzi I, discrete typing unit 1 exhibit considerablebiological diversity. Memórias do Instituto Oswaldo Cruz, 101, 585-590Search in Google Scholar
Schmunis G.A. 2007. Epidemiology of Chagas disease in non-endemic countries: the role of international migration. Memórias do Instituto Oswaldo Cruz, 102, 75-85Search in Google Scholar
Silva L.J. 1986. Desbravamento, agricultura e doenca: a doenca de Chagas no Estado de Sao Paulo. Cadernos de Saúde Pública, 2, 124-40Search in Google Scholar
Silva R.A.,Wanderley D.M.V., Domingos M.F.,Yasumaro S., Scandar S.A.S., Pauliquevis-Junior C., Sampaio S.M.P., Takaku L., Rodrigues V.L.C.C. 2006. Doenca de Chagas: notificacao de triatomineos no Estado de Sao Paulo na decada de 1990. Revista da Sociedade Brasileira de Medicina Tropical, 39, 488-94Search in Google Scholar
Silva E.O.R., Rodrigues V.L.C., Silva R.A.,Wanderley D.M.V. 2011. Programa de controle da doenca de Chagas no Estado de Sao Paulo, Brasil: o controle e a vigilancia da transmissao vetorial. Revista da Sociedade Brasileira de Medicina Tropical, 44, supl II Search in Google Scholar
Souto R.P., Zingales B. 1993. Sensitive detection and classification of Trypanosoma cruzi by amplification of a ribosomal RNA sequences. Molecular and Biochemical Parasitology, 62, 45-52Search in Google Scholar
Teixeira A.R., Nitz N., Guimaro M.C., Gomes C., Santos-Buch C.A. 2006. Chagas disease. Postgraduate Medical Journal, 82, 788-798Search in Google Scholar
Virreira M., Serrano G., Maldonado L., Svoboda M. 2006. Trypanosoma cruzi: Typing of genotype (sub) lineages in megacolon samples from bolivian patients. Acta Tropica, 100, 252-255Search in Google Scholar
Wanderley D.M.V. 1991. Vigilancia entomologica da doenca de Chagas no Estado de Sao Paulo. Revista de Saúde Pública, 25, 28-32Search in Google Scholar
Wanderley D.M.V., Silva R.A., Barbosa G.L., Rodrigues V.L.C.C. 2009. Panstrongylus megistus - um olhar sobre a especie no Estado de Sao Paulo. Revista da Sociedade Brasileira de Medicina Tropical, 42, 464Search in Google Scholar
Westenberger S.J., Barnabe C., Campbell D.A., Sturm N. 2005. Two hybridization events define the population of Trypanosoma cruzi. Genetics, 171, 527-543Search in Google Scholar
Zingales B., Miles M.A., Campbell D.A., Tibayrenc M., Macedo A.M., Teixeira M.M., Schijman A.G., Llewellyn M.S., Lages- Silva E., Machado C.R., Andrade S.G., Sturm N.R. 2012. The revised Trypanosoma cruzi subspecific nomenclature: Rationale, epidemiological relevance and research applications. Infection, Genetics and Evolution, 122, 240-253Search in Google Scholar
Zingales B. 2011. Trypanosoma cruzi: um parasita, dois parasitas ou varios parasitas da doenca de Chagas. Revista da Biologia, 6b, 44-48 Search in Google Scholar
© 2015
Articles in the same Issue
- The use of phytotherapy in diseases caused by parasitic protozoa
- Parasite communities of the elongate tigerfish Hydrocynus forskahlii (Cuvier 1819) and redbelly tilapia Tilapia zillii (Gervais 1848) from Lake Turkana, Kenya: influence of host sex and size
- Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica
- Predominance of Cryptosporidium parvum genotype among diarrheic children from Egypt as an indicator for zoonotic transmission
- Wild boar density drives Metastrongylus infection in earthworm
- The effect of trematode parthenites on the individual fecundity of Bithynia troscheli (Prosobranchia: Bithyniidae)
- Serological screening of selected microsporidia in HPV-positive women
- Cloning and expression of apyrase gene from Ancylostoma caninum in Escherechia coli
- Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil
- A new Gyrodactylus strain on brown trout (Salmo trutta) in Jänisjärvi, Russian Karelia, and a literature revision of salmonid parasites of the genus Gyrodactylus in North-Western Russia and adjacent areas
- Phylogeography analysis and molecular evolution patterns of the nematode parasite Heligmosomum mixtum based on mitochondrial DNA sequences
- Risk of human toxocarosis in Poland due to Toxocara infection of dogs and cats
- The in vitro antimalarial interaction of 9-hydroxycalabaxanthone and α-mangostin with mefloquine/artesunate
- New species of Oswaldofilaria (Nematoda; Filarioidea; Onchocercidae) and other helminths in Acanthosaura cardamomensis (Sauria; Agamidae) from Indochina Peninsula
- The pathogenic role of Blastocystis isolated from patients with irritable bowel syndrome and colitis from Iasi, Romania
- Population dynamic of two digenean species parasitizing the grass shrimp Palaemonetes argentinus Nobili 1901 (Decapoda: Palaemonidae) in a lentic environment from Argentina
- Two new Dactylogyrus species (Monogenea: Dactylogyridae) D. angorae and D. turcicus from Capoeta angorae and C. mauricii endemic fishes in Turkey
- First record of Toxoplasma gondii in Chaetophractus villosus in Argentina
- New morphological data and first description of gravid female of Cucullanus bagre Petter, 1974 (Seuratoidea: Cucullanidae) from Bagre bagre (Linnaeus, 1766) (Siluriformes: Ariidae) off Brazil
- Further studies on Toxascaris leonina (Linstow, 1902) (Ascaridida: Ascarididae) from Felis lynx (Linnaeus) and Panthera leo (Linnaeus) (Carnivora: Felidae)
- Parasitism underground: lice (Insecta: Phthiraptera) from Ctenomys talarum (Rodentia: Ctenomyidae) along its coastal distribution in Argentina
- Buxtonellosis and coccidiosis of cattles in Northern Serbia
- Effect of structurally related flavonoids from Zuccagnia punctata Cav. on Caenorhabditis elegans
- Coccidia in passerines from the Nevado de Toluca National Park, Mexico
- 10.1515/ap-2015-0025
Articles in the same Issue
- The use of phytotherapy in diseases caused by parasitic protozoa
- Parasite communities of the elongate tigerfish Hydrocynus forskahlii (Cuvier 1819) and redbelly tilapia Tilapia zillii (Gervais 1848) from Lake Turkana, Kenya: influence of host sex and size
- Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica
- Predominance of Cryptosporidium parvum genotype among diarrheic children from Egypt as an indicator for zoonotic transmission
- Wild boar density drives Metastrongylus infection in earthworm
- The effect of trematode parthenites on the individual fecundity of Bithynia troscheli (Prosobranchia: Bithyniidae)
- Serological screening of selected microsporidia in HPV-positive women
- Cloning and expression of apyrase gene from Ancylostoma caninum in Escherechia coli
- Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil
- A new Gyrodactylus strain on brown trout (Salmo trutta) in Jänisjärvi, Russian Karelia, and a literature revision of salmonid parasites of the genus Gyrodactylus in North-Western Russia and adjacent areas
- Phylogeography analysis and molecular evolution patterns of the nematode parasite Heligmosomum mixtum based on mitochondrial DNA sequences
- Risk of human toxocarosis in Poland due to Toxocara infection of dogs and cats
- The in vitro antimalarial interaction of 9-hydroxycalabaxanthone and α-mangostin with mefloquine/artesunate
- New species of Oswaldofilaria (Nematoda; Filarioidea; Onchocercidae) and other helminths in Acanthosaura cardamomensis (Sauria; Agamidae) from Indochina Peninsula
- The pathogenic role of Blastocystis isolated from patients with irritable bowel syndrome and colitis from Iasi, Romania
- Population dynamic of two digenean species parasitizing the grass shrimp Palaemonetes argentinus Nobili 1901 (Decapoda: Palaemonidae) in a lentic environment from Argentina
- Two new Dactylogyrus species (Monogenea: Dactylogyridae) D. angorae and D. turcicus from Capoeta angorae and C. mauricii endemic fishes in Turkey
- First record of Toxoplasma gondii in Chaetophractus villosus in Argentina
- New morphological data and first description of gravid female of Cucullanus bagre Petter, 1974 (Seuratoidea: Cucullanidae) from Bagre bagre (Linnaeus, 1766) (Siluriformes: Ariidae) off Brazil
- Further studies on Toxascaris leonina (Linstow, 1902) (Ascaridida: Ascarididae) from Felis lynx (Linnaeus) and Panthera leo (Linnaeus) (Carnivora: Felidae)
- Parasitism underground: lice (Insecta: Phthiraptera) from Ctenomys talarum (Rodentia: Ctenomyidae) along its coastal distribution in Argentina
- Buxtonellosis and coccidiosis of cattles in Northern Serbia
- Effect of structurally related flavonoids from Zuccagnia punctata Cav. on Caenorhabditis elegans
- Coccidia in passerines from the Nevado de Toluca National Park, Mexico
- 10.1515/ap-2015-0025