Changes in β1 integrin in renal tubular epithelial cells after intrauterine asphyxia of rabbit pups
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Bo Yu
Abstract
Objective: We investigated the role of β1 integrin in acute renal tubular injury caused by intrauterine asphyxia of neonatal rabbits by exploring the distribution and expression changes in β1 integrin and its mRNA in renal tubular epithelial cells.
Methods: A catheter was used to temporarily block the abdominal aortas of New Zealand pregnant rabbits in order to set up the intrauterine asphyxia animal model. The rabbit pups were randomly divided into control, asphyxia, and calpain inhibitor intervention groups and their renal tubular tissues were examined at 2 h after asphyxia. Immunofluorescence and in situ hybridization were used to examine the expression of β1 integrin and its mRNA, respectively. Western blot analysis was used to show the proteolysis of β1 integrin. Calpain inhibitor I was used to show the protective effect of keeping β1 integrin from being hydrolyzed after asphyxia.
Results: (1) Normally, β1 integrin was located exclusively at the basal surface of renal tubular epithelial cells. After asphyxia a large amount of β1 integrin shifted from the basal surface to the cytoplasma and the lateral and apical surfaces and its expression decreased significantly, with simultaneous damage to renal tubular integrity and structure, many exfoliated cells and cell fragments obstructed the tubular lumen. (2) The mRNA of β1 integrin was mainly expressed in the cytoplasma. After asphyxia its expression increased significantly. (3) Proteolysis of β1 integrin was evident after asphyxia, but was significantly reduced in the calpain inhibitor intervention group. Calpain inhibitor I prevented the decrease and dislocation of β1 integrin and protected renal tubular integrity and structure.
Conclusion: Intrauterine asphyxia caused proteolysis of β1 integrin, with reduced expression and depolarized distribution, leading to tubular lumen obstruction and renal tubule destruction. Damage to β1 integrin and the renal tubule was related to the activation of calpain, and calpain inhibitor curtailed these effects.
©2009 by Walter de Gruyter Berlin New York
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- Recommendations and guidelines for perinatal practice
- Perinatal echocardiography: protocols for evaluating the fetal and neonatal heart
- Review article
- Progestational agents for the prevention of preterm birth
- Original articles – Obstetrics
- High cytomegalovirus IgG avidity is a reliable indicator of past infection in patients with positive IgM detected during the first trimester of pregnancy
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