Intrauterine infection induced oligodendrocyte injury and inducible nitric oxide synthase expression in the developing rat brain
-
Ying Shen
Abstract
Aims: In order to investigate the neuropathological effect on the developing rat brain after intrauterine infection, 2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and inducible nitric oxide synthase (iNOS) were evaluated.
Methods:Escherichia coli (E. coli) was inoculated into the uterine cervix of the time-pregnant rats and controls were injected with normal saline. Immunohistochemical staining for CNPase was performed to assess oligodendrocyte injury in pup brains at postnatal day 1, 3 and 7 (P1, P3, and P7). Immunohistochemistry was used to evaluate iNOS expression and quantitative reverse transcriptase PCR to analyze iNOS mRNA expression in pup brains at P1, P3 and P7. Nitrate reductase method was used for detection of nitric oxide (NO) concentration in pup brains at P1, P3 and P7.
Results: The immunohistochemical staining for CNPase in the E. coli-treated group showed a decrease compared with the control in periventricular white matter at P7. Obvious immunohistochemical staining of iNOS was observed in periventricular white matter of the E. coli-treated pup brains at P1. The expression of iNOS mRNA in the E. coli-treated pup brains increased at P1 and P3, but there was no significant difference at P7 compared with controls. Similarly, the NO concentration increased in the E. coli-treated pup brains at P1 and P3, and no significant difference was found at P7 compared with controls.
Conclusions: The alteration of CNPase expression indicates that intrauterine infection could cause oligodendrocyte injury in the developing brain. Moreover, the increased expression of iNOS followed by the increasing NO concentration after intrauterine infection suggests that iNOS might be a key mediator between the intrauterine infection and oligodendrocyte injury in the developing brain.
©2007 by Walter de Gruyter Berlin New York
Articles in the same Issue
- European consensus guidelines on the management of neonatal respiratory distress syndrome
- Hypogastric artery ligation for severe hemorrhage in obstetric patients
- Hypogastric artery ligation: a stitch in time
- Labor induction in preeclampsia: Is misoprostol more effective than dinoprostone?
- Neurokinin B levels in maternal and umbilical cord blood in preeclamptic and normal pregnancies
- Intrauterine infection induced oligodendrocyte injury and inducible nitric oxide synthase expression in the developing rat brain
- Chaotic indices and canonical ensemble of heart rate patterns in small-for-gestational age fetuses
- The cisterna magna size in normal second-trimester fetuses
- Type and number of sites colonized by fungi and risk of progression to invasive fungal infection in preterm neonates in neonatal intensive care unit
- Serratia marcescens bacteremia – an indicator for outbreak management and heightened surveillance
- A device for adsorbing unbound, unconjugated bilirubin and its effects in vitro and in a hyperbilirubinemic newborn piglet
- The influence of thermal environment on pulmonary hemodynamic acclimation to extrauterine life in normal full-term neonates
- Justifying the “Folate trap” in folic acid fortification programs
- Immune functions after severe twin-twin transfusion syndrome
- Are recurrent hyperglycemic episodes and median blood glucose level a prognostic factor for increased morbidity and mortality in premature infants ≤1500 g?
- Gastroschisis: primary closure using umbilical cord strengthened by a polypropylene mesh
- Neonatal antibiotic prophylaxis for the prevention of early- and late-onset Group B streptococcal sepsis
- Reply
- Management of a rare cause of neonatal airway obstruction: cervical teratoma
- Congress Calendar
Articles in the same Issue
- European consensus guidelines on the management of neonatal respiratory distress syndrome
- Hypogastric artery ligation for severe hemorrhage in obstetric patients
- Hypogastric artery ligation: a stitch in time
- Labor induction in preeclampsia: Is misoprostol more effective than dinoprostone?
- Neurokinin B levels in maternal and umbilical cord blood in preeclamptic and normal pregnancies
- Intrauterine infection induced oligodendrocyte injury and inducible nitric oxide synthase expression in the developing rat brain
- Chaotic indices and canonical ensemble of heart rate patterns in small-for-gestational age fetuses
- The cisterna magna size in normal second-trimester fetuses
- Type and number of sites colonized by fungi and risk of progression to invasive fungal infection in preterm neonates in neonatal intensive care unit
- Serratia marcescens bacteremia – an indicator for outbreak management and heightened surveillance
- A device for adsorbing unbound, unconjugated bilirubin and its effects in vitro and in a hyperbilirubinemic newborn piglet
- The influence of thermal environment on pulmonary hemodynamic acclimation to extrauterine life in normal full-term neonates
- Justifying the “Folate trap” in folic acid fortification programs
- Immune functions after severe twin-twin transfusion syndrome
- Are recurrent hyperglycemic episodes and median blood glucose level a prognostic factor for increased morbidity and mortality in premature infants ≤1500 g?
- Gastroschisis: primary closure using umbilical cord strengthened by a polypropylene mesh
- Neonatal antibiotic prophylaxis for the prevention of early- and late-onset Group B streptococcal sepsis
- Reply
- Management of a rare cause of neonatal airway obstruction: cervical teratoma
- Congress Calendar
