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The effect of processing and cryopreservation on nucleated umbilical cord blood cells

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Published/Copyright: June 1, 2005
Journal of Perinatal Medicine
From the journal Volume 32 Issue 5

Abstract

Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque™ or Puregene® red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 ± 10% (average ± standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 ± 20% compared to 91 ± 11% (p < 0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen.

Keywords: Blood; cells; cryopreservation; nucleated; recovery; umbilical cord blood
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Corresponding author: Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth’s Medical Center, 736 Cambridge Street, Boston, MA 02135/U.S.A. E-mail:

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Published Online: 2005-06-01
Published in Print: 2004-09-01

© Walter de Gruyter

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  4. Evaluation of second trimester maternal serum screening for Down’s Syndrome using the Spiegelhalter-Knill-Jones (S-KJ) approach
  5. β2-adrenergic receptor gene polymorphisms and pregnancy outcome
  6. Maternal and fetal insulin-like growth factors 1 and 2 (IGF-1, IGF-2) and IGF BP-3, and their relationship to fetal acidosis at delivery
  7. Comparison of intrapartum fetal heart rate tracings in patients with neonatal seizures vs. no seizures: what are the differences?
  8. Computerized cardiotocography parameters in pregnant women affected by pregestational diabetes mellitus
  9. The effect of processing and cryopreservation on nucleated umbilical cord blood cells
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  11. Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
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https://doi.org/10.1515/JPM.2004.142
Keywords for this article
Blood; cells; cryopreservation; nucleated; recovery; umbilical cord blood

Articles in the same Issue

  1. Fetal membrane inflammatory cytokines: a switching mechanism between the preterm premature rupture of the membranes and preterm labor pathways
  2. Preeclampsia – abnormal uterine artery Doppler is related to recurrence of symptoms during the next pregnancy
  3. Mother-to-infant vertical transmission of transfusion transmitted virus in South China
  4. Evaluation of second trimester maternal serum screening for Down’s Syndrome using the Spiegelhalter-Knill-Jones (S-KJ) approach
  5. β2-adrenergic receptor gene polymorphisms and pregnancy outcome
  6. Maternal and fetal insulin-like growth factors 1 and 2 (IGF-1, IGF-2) and IGF BP-3, and their relationship to fetal acidosis at delivery
  7. Comparison of intrapartum fetal heart rate tracings in patients with neonatal seizures vs. no seizures: what are the differences?
  8. Computerized cardiotocography parameters in pregnant women affected by pregestational diabetes mellitus
  9. The effect of processing and cryopreservation on nucleated umbilical cord blood cells
  10. Etiology of cerebral palsy
  11. Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
  12. Amniotic cavity cultures, blood cultures, and surface swabs in preterm infants – useful tools for the management of early-onset sepsis?
  13. Massive hepatic infarction in preeclampsia: successful treatment with continuous hemodiafiltration and corticosteroid therapy
  14. The use of recombinant factor VIIa in a primigravida with Glanzmann’s thrombasthenia during delivery
  15. Potential harm from puncture resistant surgical gloves
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