Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
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Corrina Powell
Abstract
Objective: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood.
Methods: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, interferon (IFN)-γ and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean ± S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test.
Results: All cytokine capacities increased rapidly by 1 hour (p < 0.001), except IL-10 (p = 0.04). TNF-α peaked between 3–6 hours (1581 ± 377 pg/million WC), declining by 24 hours. Similarly, IFN-γ peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 ± 75 pg/million WC) and IL-10 (311 ± 37 pg/million WC). Overall, IFN-γ capacity was lowest (72 ± 10 pg/million WC) and IL-6 capacity was greatest (61489 ± 7059 pg/million WC).
Conclusion: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-γ production. These results may expand our knowledge of neonatal disease, etiology and management.
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Articles in the same Issue
- Fetal membrane inflammatory cytokines: a switching mechanism between the preterm premature rupture of the membranes and preterm labor pathways
- Preeclampsia – abnormal uterine artery Doppler is related to recurrence of symptoms during the next pregnancy
- Mother-to-infant vertical transmission of transfusion transmitted virus in South China
- Evaluation of second trimester maternal serum screening for Down’s Syndrome using the Spiegelhalter-Knill-Jones (S-KJ) approach
- β2-adrenergic receptor gene polymorphisms and pregnancy outcome
- Maternal and fetal insulin-like growth factors 1 and 2 (IGF-1, IGF-2) and IGF BP-3, and their relationship to fetal acidosis at delivery
- Comparison of intrapartum fetal heart rate tracings in patients with neonatal seizures vs. no seizures: what are the differences?
- Computerized cardiotocography parameters in pregnant women affected by pregestational diabetes mellitus
- The effect of processing and cryopreservation on nucleated umbilical cord blood cells
- Etiology of cerebral palsy
- Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
- Amniotic cavity cultures, blood cultures, and surface swabs in preterm infants – useful tools for the management of early-onset sepsis?
- Massive hepatic infarction in preeclampsia: successful treatment with continuous hemodiafiltration and corticosteroid therapy
- The use of recombinant factor VIIa in a primigravida with Glanzmann’s thrombasthenia during delivery
- Potential harm from puncture resistant surgical gloves
Articles in the same Issue
- Fetal membrane inflammatory cytokines: a switching mechanism between the preterm premature rupture of the membranes and preterm labor pathways
- Preeclampsia – abnormal uterine artery Doppler is related to recurrence of symptoms during the next pregnancy
- Mother-to-infant vertical transmission of transfusion transmitted virus in South China
- Evaluation of second trimester maternal serum screening for Down’s Syndrome using the Spiegelhalter-Knill-Jones (S-KJ) approach
- β2-adrenergic receptor gene polymorphisms and pregnancy outcome
- Maternal and fetal insulin-like growth factors 1 and 2 (IGF-1, IGF-2) and IGF BP-3, and their relationship to fetal acidosis at delivery
- Comparison of intrapartum fetal heart rate tracings in patients with neonatal seizures vs. no seizures: what are the differences?
- Computerized cardiotocography parameters in pregnant women affected by pregestational diabetes mellitus
- The effect of processing and cryopreservation on nucleated umbilical cord blood cells
- Etiology of cerebral palsy
- Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
- Amniotic cavity cultures, blood cultures, and surface swabs in preterm infants – useful tools for the management of early-onset sepsis?
- Massive hepatic infarction in preeclampsia: successful treatment with continuous hemodiafiltration and corticosteroid therapy
- The use of recombinant factor VIIa in a primigravida with Glanzmann’s thrombasthenia during delivery
- Potential harm from puncture resistant surgical gloves
