Inducing proliferation of human amnion epithelial and mesenchymal cells for prospective engineering of membrane repair
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N. Ochsenbein-Kölble
Abstract
Objective: To prepare a tissue engineering approach to fetal membrane repair after premature rupture of the membranes (PROM) by characterizing the proliferation potential of human amnion epithelial and mesenchymal cells from preterm and term placenta in primary culture.
Methods: Amnion epithelial and mesenchymal cells from 15 preterm (23–36 week) and 27 term placentas collected at cesarean section were separated enzymatically, characterized immunohistochemically (anti-cytokeratin 18 and anti-E-cadherin, and anti-vimentin, respectively), and their ratio determined. Proliferation on tissue culture polystyrene (TCPS) or collagen in one medium and on TCPS in four different media after 14 days was measured photometrically and compared in preterm vs. term placenta. For statistical analysis the Mann-Whitney test was used.
Results: Preterm and term epithelial : mesenchymal cell ratios were 4.3:1 and 7.8:1. Term epithelial cells proliferated similarly on TCPS or collagen. Mesenchymal cells proliferated only with fetal bovine serum (FBS). Proliferation of term amnion cells in medium containing FBS, epithelial growth factor (EGF), insulin, transferrin and triidothyronine(T3) was significantly increased (p < 0.001) compared with the other three media, and percentage proliferation was slightly higher in preterm cells.
Conclusion: Characterization of human amnion epithelial and mesenchymal cells identified the most potent proliferation-inducing medium yet. Studies of the wound-healing potential of these cells are needed, examining their behavior and proliferation on fibrin microbeads and other extracellular matrixes as the next step towards engineering membrane repair in PROM.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Bioelectrical impedance analysis in the clinical management of preeclamptic women with edema
- Decreasing resistance in the maternal uterine and peripheral arterial system is apparently unrelated to plasma and urinary levels of nitrite/nitrate and cyclic-guanosinmonophosohate during the course of normal pregnancies
- Inducing proliferation of human amnion epithelial and mesenchymal cells for prospective engineering of membrane repair
- Obstetric outcome of excessively overgrown fetuses (≥= 5000 g): a case-control study
- Computerized analysis of fetal heart rate indices during oral glucose tolerance test
- Comparison of perinatal outcome in fetuses with reverse or absent enddiastolic flow in the umbilical artery and/or fetal descending aorta
- Operative fetoscopical management of intrauterine obstructive uropathia by urethral stent
- The impact of intrapartum factors on umbilical cord blood stem cell banking
- Neonatal nucleated red blood cell count and postpartum complications in growth restricted fetuses
- Neonatal outcome of growth discordant twin gestations
- Management of unscarred ruptured uterus
- Lethargy in a newborn: lithium toxicity or lab error?
- Acute ethanol intoxication during pregnancy and consecutive fetal cardiac arrest: a case report
- Acute pancreatitis occurring in the early postpartum period: a case report
- Book Review
- Congress Calendar
