Development of DNA-based methods to identify CITES-protected timber species: a case study in the Meliaceae family
-
Aki Michael Höltken
,
Hilke Schröder
Abstract
Violation of CITES regulations in tropical timber trade necessitates the development of accurate species identification systems. The application of available methods, mostly based on visual illustrations and descriptions of wood anatomical characteristics, proved to be difficult or even impossible, particularly on lower taxonomic levels. Further, because most of the chain-of-custody documents are externally applied marks which can easily be manipulated, control methods should be based on wood features which are inherent in the wood itself. In a case study on five closely-related genera of the Meliaceae (mahogany) family, including Swietenia sp. (listed on CITES appendix II), Khaya, Entandrophragma, and Carapa sp. (legal trade timbers), this study demonstrates the process of developing DNA markers for identification purposes. A detailed sequence analysis of several non-coding cpDNA regions resulted in an assay of seven genus-specific SNP (single nucleotide polymorphism) markers. Tools have been designed that could be applied with low-cost equipment on the basis of PCR-RFLPs without the need for sequencing or capillary electrophoresis techniques. In addition, the application of the method to wood material with degraded DNA of low overall quantity is highlighted.
©2012 by Walter de Gruyter Berlin Boston
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- Fungal biodegradation of genetically modified and lignin-altered quaking aspen (Populus tremuloides Michx.)
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- Preparation of chitosan oligomers and characterization: their antifungal activities and decay resistance
- Cellular and topochemical characteristics of secondary changes in bark tissues of beech (Fagus sylvatica)
- Short Notes
- A study by X-ray photoelectron spectroscopy (XPS) of the chemistry of the surface of Scots pine (Pinus sylvestris L.) modified by friction
- Comparative termite resistance of 12 Malaysian timber species in laboratory tests
- Meetings
- 10.1515/HF.2011.185
Articles in the same Issue
- Original Papers
- Novel paper sizing agents based on renewables. Part 5: characterization of maleated oleates by ozonolysis
- Polythiophene-cellulose composites: synthesis, optical properties and homogeneous oxidative co-polymerization
- The influence of extended mercerization on some properties of carboxymethyl cellulose (CMC)
- Minimizing the effect of undesirable reactions in ECF bleaching
- A rheological description of the water vapour sorption kinetics behaviour of wood invoking a model using a canonical assembly of Kelvin-Voigt elements and a possible link with sorption hysteresis
- Changes in chemistry, color, dimensional stability and fungal resistance of Pinus radiata D. Don wood with oil heat-treatment
- Improvement of dimensional stability of wood via combination treatment: swelling with maleic anhydride and grafting with glycidyl methacrylate and methyl methacrylate
- Synthesis and properties of resol-type phenol-formaldehyde resins prepared from H2SO4- and HCl-catalyzed phenol-liquefied Cryptomeria japonica wood
- Tack and shear strength of hybrid adhesive systems made of phenol-formaldehyde, dextrin and fish glue, and acrylic pressure-sensitive adhesive
- Properties of thermally modified medium-density fibreboards
- Carbonaceous structural changes of wood induced by microwave irradiation
- Development of DNA-based methods to identify CITES-protected timber species: a case study in the Meliaceae family
- Fungal biodegradation of genetically modified and lignin-altered quaking aspen (Populus tremuloides Michx.)
- The effect of fiber dimensions on fiber network activation and tensile strength
- Preparation of chitosan oligomers and characterization: their antifungal activities and decay resistance
- Cellular and topochemical characteristics of secondary changes in bark tissues of beech (Fagus sylvatica)
- Short Notes
- A study by X-ray photoelectron spectroscopy (XPS) of the chemistry of the surface of Scots pine (Pinus sylvestris L.) modified by friction
- Comparative termite resistance of 12 Malaysian timber species in laboratory tests
- Meetings
- 10.1515/HF.2011.185
