Lipid and lignin composition of woods from different eucalypt species
-
Jorge Rencoret
Abstract
The lipid and lignin compositions of woods from the eucalypt species Eucalyptus globulus, E. nitens, E. maidenii, E. grandis, and E. dunnii have been characterized. The lipid composition was analyzed by GC and GC/MS using short- and medium-length high-temperature capillary columns, which allowed the detection of intact high-molecular-weight compounds. Similar lipid compositions were observed in all eucalypt woods, which were dominated by sitosterol, sitosterol esters and sitosteryl 3β-D-glucopyranoside. These substance classes are mainly responsible for pitch deposition during kraft pulping of eucalypt wood. However, some quantitative differences were found in the abundance of different lipid classes, with wood from E. globulus containing the lowest amounts of these pitch-forming compounds. The lignins of all eucalypt woods were analyzed in situ (without previous isolation) by pyrolysis-GC/MS. A predominance of syringyl (S) over guaiacyl (G) lignin units was observed and the S/G ratio was in the range from 2.7 to 4.1. E. globulus wood had the highest S/G ratio, a finding that, together with its low lignin content, explains its easy delignification under kraft cooking conditions.
References
Back, E.L., Allen, L.H. Pitch Control, Wood Resin and Deresination. Tappi Press, Atlanta, GA, 2000.Search in Google Scholar
Capanema, E.A., Balakshin, M.Y., Kadla, J.F. (2005) Quantitative characterization of a hardwood milled wood lignin by nuclear magnetic resonance spectroscopy. J. Agric. Food Chem.53:9639–9649.10.1021/jf0515330Search in Google Scholar
Chang, H.-M., Sarkanen, K.V. (1973) Species variation in lignin. Effect of species on the rate of kraft delignification. Tappi56:132–134.Search in Google Scholar
del Río, J.C., Gutiérrez, A., González-Vila, F.J., Martín, F., Romero, J. (1998) Characterization of organic deposits produced in the kraft pulping of Eucalyptus globulus wood. J. Chromatogr. A823:457–465.Search in Google Scholar
del Río, J.C., Romero, J., Gutiérrez, A. (2000) Analysis of pitch deposits produced in kraft pulp mills using a totally chlorine free bleaching sequence. J. Chromatogr. A874:235–245.Search in Google Scholar
del Río, J.C., Gutiérrez, A., Romero, J., Martínez, M.J., Martínez, A.T. (2001a) Identification of residual lignin markers in eucalypt kraft pulp by Py-GC-MS. J. Anal. Appl. Pyrolysis58/59:425–439.10.1016/S0165-2370(00)00126-1Search in Google Scholar
del Río, J.C., Gutiérrez, A., Martínez, M.J., Martínez, A.T. (2001b) Py-GC-MS study of Eucalyptus globulus wood treated with different fungi. J. Anal. Appl. Pyrolysis58/59:441–453.10.1016/S0165-2370(00)00184-4Search in Google Scholar
del Río, J.C., Speranza, M., Gutiérrez, A., Martínez, M.J., Martínez, A.T. (2002) Lignin attack during eucalypt wood decay by selected basidiomycetes: a Py-GC/MS study. J. Anal. Appl. Pyrolysis64:421–431.Search in Google Scholar
del Río, J.C., Rodríguez, M.J., Gutiérrez, A. (2004) Identification of intact long-chain p-hydroxycinnamate esters in leaf fibers of abaca (Musa textilis) using gas chromatography/mass spectrometry. Rapid Commun. Mass Spectrom.18:2691–2696.Search in Google Scholar
del Río, J.C., Gutiérrez, A., Hernando, M., Landín, P., Romero, J., Martínez, A.T. (2005) Determining the influence of eucalypt lignin composition in paper pulp yield using Py-GC/MS. J. Anal. Appl. Pyrolysis74:110–115.Search in Google Scholar
Evtuguin, D.V., Pascoal Neto, C., Silva, A.M.S., Domingues, P.M., Amado, F.M.L., Robert, D., Faix, O. (2001) Comprehensive study on the chemical structure of dioxane lignin from plantation Eucalyptus globulus wood. J. Agric. Food Chem.49:4252–4261.10.1021/jf010315dSearch in Google Scholar
Faix, O., Meier, D., Fortmann, I. (1990) Thermal degradation products of wood. A collection of electron of electron-impact (EI) mass spectra of monomeric lignin derived products. Holz Roh Werkst.48:351–354.Search in Google Scholar
Freire, C.S.R., Silvestre, A.J.D., Neto, C.P. (2002) Identification of new hydroxy fatty acids and ferulic acid esters in the wood of Eucalyptus globulus. Holzforschung56:143–149.10.1515/HF.2002.024Search in Google Scholar
Freire, C.S.R., Pinto, P.C.R., Santiago, A.S., Silvestre, A.J.D., Evtuguin, D.V., Neto C.P. (2006) Comparative study of lipophilic extractives of hardwoods and corresponding ECF bleached kraft pulps. Bioresources1:3–17.10.15376/biores.1.1.3-17Search in Google Scholar
González-Vila, F.J., Almendros, G., del Río, J.C., Martín, F., Gutiérrez, A., Romero, J. (1999) Ease of delignification assessment of wood from different Eucalyptus species by pyrolysis (TMAH)-GC/MS and CP/MAS 13C-NMR spectrometry. J. Anal. Appl. Pyrolysis49:295–305.10.1016/S0165-2370(98)00097-7Search in Google Scholar
Gutiérrez, A., del Río, J.C. (2001) Gas chromatography/mass spectrometry demonstration of steryl glycosides in eucalypt wood, Kraft pulp and process liquids. Rapid Commun. Mass Spectrom.15:2515–2520.10.1002/rcm.537Search in Google Scholar
Gutiérrez A., del Río J.C., González-Vila F.J., Romero J. (1998a) Variation in the composition of wood extractives from Eucalyptus globulus during seasoning. J. Wood Chem. Technol.18:439–446.10.1080/02773819809349591Search in Google Scholar
Gutiérrez, A., del Río, J.C., González-Vila, F.J., Martín, F. (1998b) Analysis of lipophilic extractives from wood and pitch deposits by solid-phase extraction and gas chromatography. J. Chromatogr. A823:449–455.10.1016/S0021-9673(98)00356-2Search in Google Scholar
Gutiérrez, A., del Río, J.C., González-Vila, F.J., Martín, F. (1999) Chemical composition of lipophilic extractives from Eucalyptus globulus Labill. wood. Holzforschung53:481–486.10.1515/HF.1999.079Search in Google Scholar
Gutiérrez, A., Romero, J., del Río, J.C. (2001a) Lipophilic extractives in process waters during manufacturing of totally chlorine free Kraft pulp from eucalypt wood. Chemosphere44:1237–1242.10.1016/S0045-6535(00)00271-XSearch in Google Scholar
Gutiérrez, A., Romero, J., del Río, J.C. (2001b) Lipophilic extractives from Eucalyptus globulus pulp during kraft cooking followed by TCF and ECF bleaching. Holzforschung55:260–264.10.1515/HF.2001.043Search in Google Scholar
Gutiérrez, A., del Río, J.C., Martínez, M.J., Martínez, A.T. (2001c) Biotechnological control of pitch troubles in paper pulp manufacturing. Trends Biotechnol.19:340–348.10.1016/S0167-7799(01)01705-XSearch in Google Scholar
Gutiérrez, A., del Río, J.C., Martínez, A.T. (2004) Chemical analysis and biological removal of wood lipids forming pitch deposits in paper pulp manufacturing. In: Methods in Biotechnology: Environmental Microbiology; Methods and Protocols; Eds. Spencer, J.F.T., Ragout de Spencer, A.L. Humana Press, Totowa, NJ. pp. 189–202.10.1385/1-59259-765-3:189Search in Google Scholar
Hillis, W.E., Sumimoto, M. (1989) Effect of extractives on pulping. In: Natural Products of Woody Plants II; Ed. Rowe, J.W. Springer-Verlag, Berlin. pp. 880–920.10.1007/978-3-642-74075-6_23Search in Google Scholar
Ona, T., Sonoda, T., Itoh K., Shibata, M. (1997) Relationship of lignin content, lignin monomeric composition and hemicellulose composition in the same trunk sought by their within-tree variations in Eucalyptus camaldulensis and E. globulus. Holzforschung51:396–404.10.1515/hfsg.1997.51.5.396Search in Google Scholar
Ralph, J., Hatfield, R.D. (1991) Pyrolysis-GC/MS characterization of forage materials. J. Agric. Food Chem.39:1426–1437.10.1021/jf00008a014Search in Google Scholar
Rencoret, J., Gutiérrez, A., del Río, J.C. (2006) Identification of three different tocopheryl ester series in wood extractives from several species of eucalyptus. In: Proceedings of the 9th European Workshop on Lignocellulosics and Pulp, Vienna. pp. 451–454.Search in Google Scholar
Rodrigues, J., Meier, D., Faix, O., Pereira, H. (1999) Determination of tree to tree variation in syringyl/guaiacyl ratio of Eucalyptus globulus wood lignin by analytical pyrolysis. J. Anal. Appl. Pyrolysis48:121–128.10.1016/S0165-2370(98)00134-XSearch in Google Scholar
Sarkanen, K.V., Hergert, H.L. (1971) Classification and Distribution. In: Lignins – Occurrence, Formation, Structure and Reactions. Eds. Sarkanen, K.V., Ludwig, C.H. Wiley, New York. pp. 43–94.Search in Google Scholar
Silvestre, A.J.D., Pereira, C.C.L., Pascoal Neto, C., Evtuguin, D.V., Duarte, A.C., Cavaleiro, J.A.S., Furtado, F.P. (1999) Chemical composition of pitch deposits from an ECF Eucalyptus globulus bleached kraft pulp mill: its relationship with wood extractives and additives in process streams. Appita J.52:375–382.Search in Google Scholar
Tappi standard (2004) T222 om-88. 2004–2005 Tappi Test Methods. Tappi Press, Atlanta, GA.Search in Google Scholar
Tsutsumi, Y., Kondo, R., Sakai, K., Imamura, H. (1995) The difference of reactivity between syringyl lignin and guaiacyl lignin in alkaline systems. Holzforschung49:423–428.10.1515/hfsg.1995.49.5.423Search in Google Scholar
Yokoi, H., Ishida, Y., Ohtani, H., Tsuge, S., Sonoda, T., Ona, T. (1999) Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis-gas chromatography. Analyst124:669–674.10.1039/a809217fSearch in Google Scholar
Yokoi, H., Nakase, T., Ishida, Y., Ohtani, H., Tsuge, S., Sonoda, T., Ona, T. (2001) Discriminative analysis of Eucalyptus camaldulensis grown from seeds of various origins based on lignin components measured by pyrolysis-gas chromatography. J. Anal. Appl. Pyrolysis57:145–152.10.1016/S0165-2370(00)00137-6Search in Google Scholar
©2007 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Characterization of thin water layers in pulp by tritium exchange. Part 1: Methods development
- Characterization of thin water layers in pulp by tritium exchange. Part 2: Effect of refining on water absorption
- Characterizing wood fiber and particle length with a mixture distribution and a segmented distribution
- Effect of photolysis on 17th/18th century paper
- Changes in the cell wall volume of a number of wood species due to reaction with acetic anhydride
- A novel simple route to wood acetylation by transesterification with vinyl acetate
- Formation and properties of nanocomposites made up from solid aspen wood, melamine-urea-formaldehyde, and clay
- Ellagitannins from Portuguese oak wood (Quercus pyrenaica Willd.) used in cooperage: influence of geographical origin, coarseness of the grain and toasting level
- Epimeric phenylpropanoid glycosides from inner bark of Paulownia coreana Uyeki
- Lipid and lignin composition of woods from different eucalypt species
- A hysteresis model suitable for numerical simulation of moisture content in wood
- Simple estimation of critical stress intensity factors of wood by tests with double cantilever beam and three-point end-notched flexure
- Screening fungi tolerant to Western red cedar (Thuja plicata Donn) extractives. Part 1. Mild extraction by ultrasonication and quantification of extractives by reverse-phase HPLC
- Screening fungi tolerant to Western red cedar (Thuja plicata Donn) extractives. Part 2. Development of a feeder strip assay
- Contribution of hardness to the natural resistance of a range of wood species to attack by the marine borer Limnoria
- Biodeterioration of treated Pinus radiata timber by Australian decay fungi and the termite Coptotermes acinaciformis in laboratory bioassays and field conditions
- Development of an accelerated soil-contact decay test
- Obituary Dr. Bo Hortling (1942–2006)
Articles in the same Issue
- Characterization of thin water layers in pulp by tritium exchange. Part 1: Methods development
- Characterization of thin water layers in pulp by tritium exchange. Part 2: Effect of refining on water absorption
- Characterizing wood fiber and particle length with a mixture distribution and a segmented distribution
- Effect of photolysis on 17th/18th century paper
- Changes in the cell wall volume of a number of wood species due to reaction with acetic anhydride
- A novel simple route to wood acetylation by transesterification with vinyl acetate
- Formation and properties of nanocomposites made up from solid aspen wood, melamine-urea-formaldehyde, and clay
- Ellagitannins from Portuguese oak wood (Quercus pyrenaica Willd.) used in cooperage: influence of geographical origin, coarseness of the grain and toasting level
- Epimeric phenylpropanoid glycosides from inner bark of Paulownia coreana Uyeki
- Lipid and lignin composition of woods from different eucalypt species
- A hysteresis model suitable for numerical simulation of moisture content in wood
- Simple estimation of critical stress intensity factors of wood by tests with double cantilever beam and three-point end-notched flexure
- Screening fungi tolerant to Western red cedar (Thuja plicata Donn) extractives. Part 1. Mild extraction by ultrasonication and quantification of extractives by reverse-phase HPLC
- Screening fungi tolerant to Western red cedar (Thuja plicata Donn) extractives. Part 2. Development of a feeder strip assay
- Contribution of hardness to the natural resistance of a range of wood species to attack by the marine borer Limnoria
- Biodeterioration of treated Pinus radiata timber by Australian decay fungi and the termite Coptotermes acinaciformis in laboratory bioassays and field conditions
- Development of an accelerated soil-contact decay test
- Obituary Dr. Bo Hortling (1942–2006)