High-performance liquid chromatographic analysis of soluble and total oxalate in Ca- and Mg-amended liquid cultures of three wood decay fungi
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Jonathan S. Schilling
Abstract
Two brown-rot wood decay fungi, Fomitopsis pinicola and Meruliporia incrassata, and the white-rot species Phanerochaete chrysosporium were grown for 4 weeks in liquid culture at 0.35, 0.70, 1.05, and 5.00 mM calcium (Ca) and 1.35 and 2.70 mM magnesium (Mg) concentrations. Soluble and total oxalate levels were quantified using a revised ion-exchange HPLC protocol developed specifically for resolving oxalate and other organic acid anions from medium components. Total oxalate concentrations in brown-rot filtrate were not significantly different among treatments; however, soluble oxalate decreased significantly with increasing Ca concentration. Higher Mg concentrations increased soluble oxalate levels only slightly. There was a significant decrease in medium pH at 5.00 mM Ca for all species, as well as an apparent increase in decarboxylation activity in brown-rot fungi. Total and soluble oxalate levels in the white-rot cultures were generally below detection for all treatments. The results show a significant influence of Ca on soluble oxalate concentrations not seen previously in the brown-rot species Postia placenta.
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© Walter de Gruyter
Articles in the same Issue
- Subject Index
- Contents
- Species Index
- Author Index
- Monoethanolamine (MEA) pulping of beech and spruce wood for production of dissolving pulp
- Voltammetric analysis of the bleachability of softwood kraft pulps
- Alkaline degradation of model compounds related to beech xylan
- Effect of UV radiation on the carbonyl distribution in different pulps
- Changes in the lignin-carbohydrate complex in softwood kraft pulp during kraft and oxygen delignification
- Carbohydrate structures in residual lignin-carbohydrate complexes of spruce and pine pulp
- Ozonation of pine kraft lignin in alkaline solution. Part 1: Ozonation, characterization of kraft lignin and its ozonated preparations
- Ozonation of pine kraft lignin in alkaline solution. Part 2: Surface active properties of the ozonated kraft lignins
- Lignin reactions in oxygen delignification catalysed by Mn(II)-substituted molybdovanadophosphate polyanion
- Bioactive phenolic substances in industrially important tree species. Part 2: Knots and stemwood of fir species
- Evaluation of hydrocarbon emissions from heart- and sapwood of Scots pine using a laboratory-scale wood drier
- Effect of pulsating tension-torsion combined loading on fatigue behavior in wood
- Effects of density and microfibril orientation on the vertical variation of low-stiffness wood in radiata pine butt logs
- Determination of the natural durability of larch wood (Larix decidua Mill.) from the Western Italian Alps
- High-performance liquid chromatographic analysis of soluble and total oxalate in Ca- and Mg-amended liquid cultures of three wood decay fungi
- Artificial weathering of tropical woods. Part 1: Changes in wettability and Artificial weathering of tropical woods. Part II: Color change
Articles in the same Issue
- Subject Index
- Contents
- Species Index
- Author Index
- Monoethanolamine (MEA) pulping of beech and spruce wood for production of dissolving pulp
- Voltammetric analysis of the bleachability of softwood kraft pulps
- Alkaline degradation of model compounds related to beech xylan
- Effect of UV radiation on the carbonyl distribution in different pulps
- Changes in the lignin-carbohydrate complex in softwood kraft pulp during kraft and oxygen delignification
- Carbohydrate structures in residual lignin-carbohydrate complexes of spruce and pine pulp
- Ozonation of pine kraft lignin in alkaline solution. Part 1: Ozonation, characterization of kraft lignin and its ozonated preparations
- Ozonation of pine kraft lignin in alkaline solution. Part 2: Surface active properties of the ozonated kraft lignins
- Lignin reactions in oxygen delignification catalysed by Mn(II)-substituted molybdovanadophosphate polyanion
- Bioactive phenolic substances in industrially important tree species. Part 2: Knots and stemwood of fir species
- Evaluation of hydrocarbon emissions from heart- and sapwood of Scots pine using a laboratory-scale wood drier
- Effect of pulsating tension-torsion combined loading on fatigue behavior in wood
- Effects of density and microfibril orientation on the vertical variation of low-stiffness wood in radiata pine butt logs
- Determination of the natural durability of larch wood (Larix decidua Mill.) from the Western Italian Alps
- High-performance liquid chromatographic analysis of soluble and total oxalate in Ca- and Mg-amended liquid cultures of three wood decay fungi
- Artificial weathering of tropical woods. Part 1: Changes in wettability and Artificial weathering of tropical woods. Part II: Color change