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High-performance liquid chromatographic analysis of soluble and total oxalate in Ca- and Mg-amended liquid cultures of three wood decay fungi

  • Jonathan S. Schilling and Jody Jellison
Published/Copyright: June 1, 2005
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Holzforschung
From the journal Volume 58 Issue 6

Abstract

Two brown-rot wood decay fungi, Fomitopsis pinicola and Meruliporia incrassata, and the white-rot species Phanerochaete chrysosporium were grown for 4 weeks in liquid culture at 0.35, 0.70, 1.05, and 5.00 mM calcium (Ca) and 1.35 and 2.70 mM magnesium (Mg) concentrations. Soluble and total oxalate levels were quantified using a revised ion-exchange HPLC protocol developed specifically for resolving oxalate and other organic acid anions from medium components. Total oxalate concentrations in brown-rot filtrate were not significantly different among treatments; however, soluble oxalate decreased significantly with increasing Ca concentration. Higher Mg concentrations increased soluble oxalate levels only slightly. There was a significant decrease in medium pH at 5.00 mM Ca for all species, as well as an apparent increase in decarboxylation activity in brown-rot fungi. Total and soluble oxalate levels in the white-rot cultures were generally below detection for all treatments. The results show a significant influence of Ca on soluble oxalate concentrations not seen previously in the brown-rot species Postia placenta.

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Published Online: 2005-06-01
Published in Print: 2004-10-01

© Walter de Gruyter

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  3. Species Index
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