Application of confocal Raman spectroscopy for the topochemical distribution of lignin and cellulose in plant cell walls of beech wood (Fagus sylvatica L.) compared to UV microspectrophotometry
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Thomas Röder
Abstract
In a comparative study, the topochemical distribution of lignin in individual cell wall layers of beech wood tissue was determined by confocal Raman spectroscopy and scanning UV microspectrophotometry. The Raman technique was additionally applied to the determination of cellulose content in individual wall layers. In good agreement, the two methods showed maxima of lignin distribution in middle lamellae and cell corners, along with a minimum of cellulose content.
References
Agarwal, U.P. (1999) An overview of Raman spectroscopy as applied to lignocellulosic materials. In: Advances in Lignocellulosics Characterization. Ed. Argyropopoulos, D.S. Atlanta, USA: TAPPI Press. pp. 201–225.Search in Google Scholar
Agarwal, U.P., Ralph, S.A. (1997) FT-Raman Spectroscopy of Wood: Identifying contributions of lignin and carbohydrate polymers in the spectrum of black spruce (Picea mariana). Appl. Spectrosc.51:1648–1655.10.1366/0003702971939316Search in Google Scholar
Barbillat, J., Silva, E., Lenain, B., Manfait, M., Sharonov, S., Valisa, P. (1994) Raman confocal spectral imaging. In: ICORS Hong Kong 94. Eds. Yu, N.T., Li, X. Y. Wiley, Singapore. pp. 1102–1106.Search in Google Scholar
Bond, J.S., Atalla, R.H. (1999) A Raman microprobe investigation of the molecular architecture of loblolly pine tracheids. In: Proceedings of the 10th international symposium on wood and pulping chemistry, main symposium; 1999 June 7–10; Yokohama, Japan. Atlanta, USA: TAPPI Press. pp. 96–101.Search in Google Scholar
Everall, N.J. (2000) Modelling and measuring the effect of refraction on the depth resolution of confocal Raman microscopy. Appl. Spectrosc.54:773–782.10.1366/0003702001950382Search in Google Scholar
Fengel, D., Wegener, G. Wood–Chemistry, Ultrastructure, Reactions. De Gruyter, Berlin, 1989, p. 613.Search in Google Scholar
Fergus, B.J., Goring, D.A.I. (1970) The location of guaiacyl and syringyl lignins in birch xylem tissue. Holzforschung24:113–117.10.1515/hfsg.1970.24.4.113Search in Google Scholar
Fergus, B.J., Procter, A.R., Scott, J.A.N., Goring, D.A.I. (1969) The distribution of lignin in spruce wood as determined by ultraviolet microscopy. Wood Sci. Technol.3:117–138.10.1007/BF00639636Search in Google Scholar
Fukazawa, K. (1992) Ultraviolet microscopy. In: Methods in Lignin Chemistry. Eds. Lin, S.Y., Dence, C.W. Springer-Verlag, Berlin. pp. 110–121.10.1007/978-3-642-74065-7_8Search in Google Scholar
Koch, G., Kleist, G. (2001) Application of scanning UV microspectrophotometry to localize lignins and phenolic extractives in plant cell walls. Holzforschung55:563–567.10.1515/HF.2001.091Search in Google Scholar
© Walter de Gruyter
Articles in the same Issue
- The effects of initial spacing on wood density, fibre and pulp properties in jack pine (Pinus banksiana Lamb.)
- An improved procedure for isolation of residual lignins from hardwood kraft pulps
- Small angle scattering in the Porod region from hydrated paper sheets at varying humidities
- Application of confocal Raman spectroscopy for the topochemical distribution of lignin and cellulose in plant cell walls of beech wood (Fagus sylvatica L.) compared to UV microspectrophotometry
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- On-line study of lignin behaviour in dilute alkaline solution by the SEC-UV method
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- New glucosides from Eucalyptus globulus wood, bark and kraft pulps
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Articles in the same Issue
- The effects of initial spacing on wood density, fibre and pulp properties in jack pine (Pinus banksiana Lamb.)
- An improved procedure for isolation of residual lignins from hardwood kraft pulps
- Small angle scattering in the Porod region from hydrated paper sheets at varying humidities
- Application of confocal Raman spectroscopy for the topochemical distribution of lignin and cellulose in plant cell walls of beech wood (Fagus sylvatica L.) compared to UV microspectrophotometry
- UV-microscopic analysis of acetylated spruce and birch cell walls
- On-line study of lignin behaviour in dilute alkaline solution by the SEC-UV method
- Extracellular diffusion pathway for heartwood substances in Albizia julibrissin Durazz.
- New glucosides from Eucalyptus globulus wood, bark and kraft pulps
- Nondestructive characterization of wood by monitoring of local elastic anisotropy and dynamic nonlinearity
- Chemical reaction of maritime pine sapwood (Pinus pinaster Soland) with alkoxysilane molecules: A study of chemical pathways
- Thermal forming of chemically modified wood to make high-performance plastic-like wood composites
- Eco-composite from poly(lactic acid) and bamboo fiber
- Bamboo fiber (BF)-filled poly(butylenes succinate) bio-composite – Effect of BF-e-MA on the properties and crystallization kinetics
- Dimensional changes in Corsican pine (Pinus nigra Arnold) modified with acetic anhydride measured using a helium pycnometer
- Microwave-enhanced release of formaldehyde from plywood
- Artificial weathering of tropical woods. Part 1: Changes in wettability
- Artificial weathering of tropical woods. Part 2: Color change
- Inhibition of fungal degradation of wood by 2-hydroxypyridine-N-oxide
- Microwave treatment to accelerate fixation of copper-ethanolamine (Cu-EA) treated wood
